Phenotypes associated with this allele

• Allele Symbol: Pyy^tm1(cre)Hhzg
• Allele Name: targeted mutation 1, H Herzog
• Allele ID: MGI:3664105
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pyy^tm1(cre)Hhzg/Pyy^tm1(cre)Hhzg	involves: 129	MGI:3664650

Genotype
MGI:3664650

hm1

• Allelic Composition: Pyy^tm1(cre)Hhzg/Pyy^tm1(cre)Hhzg
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Greater bone mass in Pyy^tm1(cre)Hhzg/Pyy^tm1(cre)Hhzg mice

growth/size/body

increased lean body mass ( J:111480 )

• both 14-week-old male and female mice show a significant increase in whole body lean mass

abnormal body weight ( J:111480 )

• male mutants do not show significant changes is body weight on a high-fat diet from 4 weeks of age compared to wild-type mice

increased body weight ( J:111480 )

• on a normal chow diet, female mutants from 4 to 28 weeks of age have significantly increased body weights compared to wild-type (area under curve (AUC): wild-type, 505g/24 weeks vs Pyy-null, 554g/24 weeks)

• females show a marked increase in body weight between 15 and 28 weeks (AUC: mutants 344 g/15-28 weeks; wild-type 307.6 g/ 15-28 weeks)

increased susceptibility to age related obesity ( J:111480 )

• female mutants, not males, develop late-onset obesity

homeostasis/metabolism

increased circulating testosterone level ( J:111480 )

• in regular chow-fed male mutants, serum testosterone levels are elevated to a statistically significant degree

increased circulating insulin level ( J:111480 )

• male and female chow-fed animals show higher serum insulin levels than controls during glucose tolerance test period

• fasting and glucose-induced serum insulin levels are elevated significantly above those observed in wild-type mice fed a high fat diet (mutant >200 pmol/l/min vs wild-type ~150 pmol/l/min)

increased circulating insulin-like growth factor I level ( J:111480 )

• male mutants have significantly higher serum levels of IGF-1 when fed a normal chow diet

impaired glucose tolerance ( J:111480 )

• on a high-fat diet, male mutants are less glucose-tolerant than wild-type but difference disappears when data are normalized for bodyweight

adipose tissue

increased white adipose tissue amount ( J:111480 )

• weight of all white fat adipose tissue deposits in females are increased at 28 weeks of age, consistent with increase in body fat

decreased total body fat amount ( J:111480 )

• females show a decrease in whole body fat mass at 14 weeks

increased total body fat amount ( J:111480 )

• chow-fed females show a significant 50% increase in fat mass at 28 weeks of age

increased gonadal fat pad weight ( J:111480 )

• males fed on a high-fat diet from 4 weeks of age show significant increases in weight of gonadal white fat deposits

behavior/neurological

behavior/neurological phenotype ( J:111480 )

N • mutants show no alterations in motor activity over a 14 day observation period

nervous system

abnormal hypothalamus physiology ( J:111480 )

• 14-week old female mutants show a 51% increase in Pomc expression vs controls in the arcuate nucleus consistent with reduced food intake, while males show a 21% decrease in Npy expression and a 25% increase in Pomc, associated with increased lean mass and serum hormone levels; Ghrh is increased 32% in the ventromedial hypothalamic nucleus of mutants

skeleton

abnormal lumbar vertebrae morphology ( J:189512 )

• increase in lumbar bone mineral density and content in males and females

• increase in cancellous bone volume in the vertebral body in the females

abnormal bone structure ( J:189512 )

increased bone mineral content ( J:189512 )

• significant increase in whole body BMC in females at 14 weeks of age

• increase in lumbar BMC in males and females

increased bone mineral density ( J:189512 )

• significant increase in whole body BMD in females at 14 weeks of age

increased bone mineral density of lumbar vertebrae ( J:189512 )

• increase in lumbar BMD in males and females

increased trabecular bone volume ( J:189512 )

• increase in cancellous bone volume in the lumbar vertebral body in the females

• increase in cancellous bone volume in the distal femoral metaphysis in males and females

abnormal bone mineralization ( J:189512 )

• increase in mineral apposition rate in the distal femoral metaphysis and in the mid femur