Phenotypes associated with this allele

• Allele Symbol: Hspa5^tm1.1Alee
• Allele Name: targeted mutation 1.1, Amy S Lee
• Allele ID: MGI:3664111
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hspa5^tm1.1Alee/Hspa5^tm1.1Alee	involves: 129 * C57BL/6 * FVB/N	MGI:3712814
cn2	Hspa5^tm1Alee/Hspa5^tm1.1Alee Pten^tm1Hwu/Pten^tm1Hwu Tg(Pbsn-cre)4Prb/?	involves: 129 * 129S4/SvJae * C57BL/6 * DBA/2	MGI:4943512

Genotype
MGI:3712814

hm1

• Allelic Composition: Hspa5^tm1.1Alee/Hspa5^tm1.1Alee
• Genetic Background: involves: 129 * C57BL/6 * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality at implantation, complete penetrance ( J:111416 )

• most embryos survive to E3.5, while no homozygous embryos are recovered at E7.5: only empty deciduas are observed, indicating embryo degeneration and resorption shortly after E3.5

embryo

abnormal inner cell mass apoptosis ( J:111416 )

• there is a dramatic increase in apoptotic cell death in inner cell mass after 24 hours in culture compared to almost none in wild-type or heterozygous embryo culture

absent inner cell mass proliferation ( J:111416 )

• cultured E3.5 embryos fail to expand when cultured for 5 days after zona pellucida was mechanically removed

• after 1 day in culture, only 7% of cells show BrdU uptake vs 60% of wild-type cells

failure of blastocyst to hatch from the zona pellucida ( J:111416 )

abnormal blastocyst morphology ( J:111416 )

• after 96 hours in culture, blastocysts appear shrunken compared to wild-type

cellular

abnormal inner cell mass apoptosis ( J:111416 )

• there is a dramatic increase in apoptotic cell death in inner cell mass after 24 hours in culture compared to almost none in wild-type or heterozygous embryo culture

absent inner cell mass proliferation ( J:111416 )

• cultured E3.5 embryos fail to expand when cultured for 5 days after zona pellucida was mechanically removed

• after 1 day in culture, only 7% of cells show BrdU uptake vs 60% of wild-type cells

reproductive system

empty decidua capsularis ( J:111416 )

• only empty deciduas are observed

Genotype
MGI:4943512

cn2

• Allelic Composition: Hspa5^tm1Alee/Hspa5^tm1.1Alee; Pten^tm1Hwu/Pten^tm1Hwu; Tg(Pbsn-cre)4Prb/?
• Genetic Background: involves: 129 * 129S4/SvJae * C57BL/6 * DBA/2

neoplasm

neoplasm ( J:142106 )

N • mice do not develop detectable cancerous or precancerous lesions in the prostate