Phenotypes associated with this allele

• Allele Symbol: Gmnn^{Gt(XT0615)Wtsi}
• Allele Name: gene trap XT0615, Wellcome Trust Sanger Institute
• Allele ID: MGI:3664114
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gmnn^Gt(XT0615)Wtsi/Gmnn^Gt(XT0615)Wtsi	involves: 129P2/OlaHsd	MGI:6470677

Genotype
MGI:6470677

hm1

• Allelic Composition: Gmnn^{Gt(XT0615)Wtsi}/Gmnn^{Gt(XT0615)Wtsi}
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality before implantation, complete penetrance ( J:110696 )

• no homozygous embryos are detected at E15 or E8.5-9.5; absence of resorbed embryos or empty deciduas suggests preimplantation lethality

embryo

abnormal preimplantation embryo development ( J:110696 )

embryonic growth arrest ( J:110696 )

• at E3.5, 70/296 embryos are abnormal, consisting of only eight cells whereas 226/296 had developed into blastocysts consisting of at least 32 cells

absent inner cell mass ( J:110696 )

• mutant embryos consist of TGCs that can form 8-cell embryos resembling blastocysts with blastocoelic cavities but lacking inner cell mass (ICM)

abnormal trophectoderm morphology ( J:110696 )

• in mutant embryos, all cells become committed to trophectoderm lineage

cellular

abnormal cell nucleus morphology ( J:110696 )

• nuclei of eight-cell arrested embryos are grossly enlarged and contain three times more DNA than nuclei from wild-type blastocysts at E3.5

abnormal mitosis ( J:110696 )

• mutant cells undergo endoreduplication, resulting in >3-fold DNA content in mutant nuclei

abnormal cell differentiation ( J:110696 )

• all cells lose pleuripotency, endoreduplicate, and consist exclusively of differentiated trophoblast giant cells (TGCs)