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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tg(CAG-EGFP,Acr-EGFP)2Osb
transgene insertion 2, Research Institute for Microbial Diseases, Osaka University
MGI:3665168
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
 		Phf7em2Osb/Phf7em2Osb
Tg(CAG-EGFP,Acr-EGFP)2Osb/0 		involves: 129S2/SvPas * C57BL/6NSlc MGI:6690604
cx2
 		Zfp541em1Osb/Zfp541em1Osb
Tg(CAG-EGFP,Acr-EGFP)2Osb/0 		involves: 129S2/SvPas * C57BL/6NSlc MGI:6759016
cx3
 		Tex101tm1Osb/Tex101tm1Osb
Tg(CAG-EGFP,Acr-EGFP)2Osb/Tg(CAG-EGFP,Acr-EGFP)2Osb 		involves: C57BL/6NSlc MGI:5508227


Genotype
MGI:6690604
cx1
Allelic
Composition		 Phf7em2Osb/Phf7em2Osb
Tg(CAG-EGFP,Acr-EGFP)2Osb/0
Genetic
Background		 involves: 129S2/SvPas * C57BL/6NSlc
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Phf7em2Osb mutation (0 available); any Phf7 mutation (27 available)
Tg(CAG-EGFP,Acr-EGFP)2Osb mutation (5 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal sperm nucleus morphology ( J:298823 )
• defective histone to protamine exchange during spermiogenesis
decreased fertilization frequency ( J:298823 )
• during in vitro fertilization

cellular
abnormal sperm nucleus morphology ( J:298823 )
• defective histone to protamine exchange during spermiogenesis




Genotype
MGI:6759016
cx2
Allelic
Composition		 Zfp541em1Osb/Zfp541em1Osb
Tg(CAG-EGFP,Acr-EGFP)2Osb/0
Genetic
Background		 involves: 129S2/SvPas * C57BL/6NSlc
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(CAG-EGFP,Acr-EGFP)2Osb mutation (5 available)
Zfp541em1Osb mutation (2 available); any Zfp541 mutation (48 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal spermatid morphology ( J:306305 )
• round spermatids virtually absent from seminiferous tubules
abnormal male meiosis ( J:306305 )
• diffuse location of Sycp1 and Sycp3 synaptonemal complex proteins outside of synaptonemal complex axis in late pachytene spermatocytes
abnormal X-Y chromosome synapsis during male meiosis ( J:306305 )
• asynapsis of X and Y chromosomes in early pachytene spermatocytes
arrest of male meiosis ( J:306305 )
• lack of diplotene spermatocytes
abnormal synaptonemal complex ( J:306305 )
• diffuse location of Sycp1 and Sycp3 synaptonemal complex proteins outside of synaptonemal complex axis in late pachytene spermatocytes
increased primordial germ cell apoptosis ( J:306305 )
• in stage II-V, VI-IX and X-XII seminiferous tubules

cellular
abnormal spermatid morphology ( J:306305 )
• round spermatids virtually absent from seminiferous tubules
abnormal male meiosis ( J:306305 )
• diffuse location of Sycp1 and Sycp3 synaptonemal complex proteins outside of synaptonemal complex axis in late pachytene spermatocytes
abnormal X-Y chromosome synapsis during male meiosis ( J:306305 )
• asynapsis of X and Y chromosomes in early pachytene spermatocytes
arrest of male meiosis ( J:306305 )
• lack of diplotene spermatocytes
abnormal synaptonemal complex ( J:306305 )
• diffuse location of Sycp1 and Sycp3 synaptonemal complex proteins outside of synaptonemal complex axis in late pachytene spermatocytes
increased primordial germ cell apoptosis ( J:306305 )
• in stage II-V, VI-IX and X-XII seminiferous tubules




Genotype
MGI:5508227
cx3
Allelic
Composition		 Tex101tm1Osb/Tex101tm1Osb
Tg(CAG-EGFP,Acr-EGFP)2Osb/Tg(CAG-EGFP,Acr-EGFP)2Osb
Genetic
Background		 involves: C57BL/6NSlc
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tex101tm1Osb mutation (1 available); any Tex101 mutation (20 available)
Tg(CAG-EGFP,Acr-EGFP)2Osb mutation (5 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:197323 )
N
• testis weights and histology are normal
• spermatozoa are morphologically normal
• mating behavior, ejaculation, and vaginal plug formation are all normal
impaired sperm migration in female genital tract ( J:197323 )
• defect in the ability of sperm to migrate into the oviduct
• capacitated sperm deposited directly into the oviduct fertilize eggs normally
abnormal hyperactivated sperm motility ( J:197323 )
• percentage of hyperactivated spermatozoa is higher than in wild-type cells
• however, sperm motility is normal
abnormal acrosome reaction ( J:197323 )
• ratio of acrosome-reacted and acrosome intact spermatozoa is normal for the first 90 min of incubation but increased after 180 min of incubation
impaired binding of sperm to zona pellucida ( J:197323 )
• despite frequent collisions, zona binding ability of spermatozoa to cumulus free eggs is nearly lost
• fertilizing ability is normal when cumulus-intact eggs are used

behavior/neurological
behavior/neurological phenotype ( J:197323 )
N
• mating behavior is normal

cellular
impaired sperm migration in female genital tract ( J:197323 )
• defect in the ability of sperm to migrate into the oviduct
• capacitated sperm deposited directly into the oviduct fertilize eggs normally
abnormal hyperactivated sperm motility ( J:197323 )
• percentage of hyperactivated spermatozoa is higher than in wild-type cells
• however, sperm motility is normal




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
06/12/2024
MGI 6.13 		The Jackson Laboratory