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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Arhgef1tm1.1Rmt
targeted mutation 1.1, Raul M Torres
MGI:3686863
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Arhgef1tm1.1Rmt/Arhgef1tm1.1Rmt C57BL/6-Arhgef1tm1.1Rmt MGI:3687247
cx2
 		Ightm2Cgn/Igh+
Rr158tm1Cgn/Rr158tm1Cgn
Arhgef1tm1.1Rmt/Arhgef1tm1.1Rmt 		involves: 129P2/OlaHsd * C57BL/6 MGI:3687248


Genotype
MGI:3687247
hm1
Allelic
Composition		 Arhgef1tm1.1Rmt/Arhgef1tm1.1Rmt
Genetic
Background		 C57BL/6-Arhgef1tm1.1Rmt
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Arhgef1tm1.1Rmt mutation (0 available); any Arhgef1 mutation (73 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased B cell number ( J:113295 )
• variable decrease in peripheral B cells from 20% in lymph nodes to 50% in the spleen
decreased B-1 B cell number ( J:113295 )
• 80% decrease in peritoneal B1 cells
decreased follicular B cell number ( J:113295 )
• about a 50% reduction in follicular B cells in spleen
decreased marginal zone B cell number ( J:113295 )
• about a 50% reduction in marginal zone B cells in spleen
decreased IgM level ( J:113295 )
• increase in response to T cell dependent antigen stimulation is less than 20% of wild-type; however unstimulated levels of IgM and IgG1 are similar to wild-type
• no difference is seen in antigen specific IgM response between sham-treated and asplenic mice, unlike in wild-type mice where removal of the spleen significantly reduces antigen specific IgM response
abnormal marginal zone B cell physiology ( J:113295 )
• prolonged adhesion of marginal zone B cells to integrin-ligands both in vitro and in vivo
• increase in the proportion of cells that migrate towards high sphingosine 1-phosphate concentrations
• 24 hours after immunization with LPS or LPS plus T cell dependent antigen (4-hydroxy-3-mitrophenylacetyl-chicken gamma-globulin, NP-CG) a significant fraction of B cells remain in the marginal zone unlike in wild-type mice

hematopoietic system
decreased B cell number ( J:113295 )
• variable decrease in peripheral B cells from 20% in lymph nodes to 50% in the spleen
decreased B-1 B cell number ( J:113295 )
• 80% decrease in peritoneal B1 cells
decreased follicular B cell number ( J:113295 )
• about a 50% reduction in follicular B cells in spleen
decreased marginal zone B cell number ( J:113295 )
• about a 50% reduction in marginal zone B cells in spleen
decreased IgM level ( J:113295 )
• increase in response to T cell dependent antigen stimulation is less than 20% of wild-type; however unstimulated levels of IgM and IgG1 are similar to wild-type
• no difference is seen in antigen specific IgM response between sham-treated and asplenic mice, unlike in wild-type mice where removal of the spleen significantly reduces antigen specific IgM response
abnormal marginal zone B cell physiology ( J:113295 )
• prolonged adhesion of marginal zone B cells to integrin-ligands both in vitro and in vivo
• increase in the proportion of cells that migrate towards high sphingosine 1-phosphate concentrations
• 24 hours after immunization with LPS or LPS plus T cell dependent antigen (4-hydroxy-3-mitrophenylacetyl-chicken gamma-globulin, NP-CG) a significant fraction of B cells remain in the marginal zone unlike in wild-type mice




Genotype
MGI:3687248
cx2
Allelic
Composition		 Ightm2Cgn/Igh+
Rr158tm1Cgn/Rr158tm1Cgn
Arhgef1tm1.1Rmt/Arhgef1tm1.1Rmt
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Arhgef1tm1.1Rmt mutation (0 available); any Arhgef1 mutation (73 available)
Ightm2Cgn mutation (1 available); any Igh mutation (44 available)
Rr158tm1Cgn mutation (0 available); any Rr158 mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal B cell migration ( J:113295 )
• increase in the proportion of cells that migrate towards high sphingosine 1-phosphate (S1P) concentrations
• reduction in migratory response towards S1P induced by LPS treatment is decreased compared to wild-type
• immunization with a low dose of T cell dependent antigen (NP-CG) fails to suppress responsiveness to S1P unlike in wild-type mice; however, treatment with a high concentration of antigen does reduce S1P responsiveness

immune system
abnormal B cell migration ( J:113295 )
• increase in the proportion of cells that migrate towards high sphingosine 1-phosphate (S1P) concentrations
• reduction in migratory response towards S1P induced by LPS treatment is decreased compared to wild-type
• immunization with a low dose of T cell dependent antigen (NP-CG) fails to suppress responsiveness to S1P unlike in wild-type mice; however, treatment with a high concentration of antigen does reduce S1P responsiveness

hematopoietic system
abnormal B cell migration ( J:113295 )
• increase in the proportion of cells that migrate towards high sphingosine 1-phosphate (S1P) concentrations
• reduction in migratory response towards S1P induced by LPS treatment is decreased compared to wild-type
• immunization with a low dose of T cell dependent antigen (NP-CG) fails to suppress responsiveness to S1P unlike in wild-type mice; however, treatment with a high concentration of antigen does reduce S1P responsiveness




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory