Phenotypes associated with this allele

• Allele Symbol: Pycard^tm1Flv
• Allele Name: targeted mutation 1, Richard A Flavell
• Allele ID: MGI:3686870
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pycard^tm1Flv/Pycard^tm1Flv	B6.129S5-Pycard^tm1Flv	MGI:5085869
hm2	Pycard^tm1Flv/Pycard^tm1Flv	involves: 129S5/SvEvBrd * C57BL/6	MGI:3687250
cn3	Nlrp3^tm1Hhf/Nlrp3^+ Pycard^tm1Flv/Pycard^tm1Flv Lyz2^tm1(cre)Ifo/Lyz2^+	involves: 129 * C57BL/6	MGI:3850058
cx4	Pycard^tm1Flv/Pycard^tm1Flv Slc11a1^s/Slc11a1^s	B6.129S5-Pycard^tm1Flv	MGI:3722762

Genotype
MGI:5085869

hm1

• Allelic Composition: Pycard^tm1Flv/Pycard^tm1Flv
• Genetic Background: B6.129S5-Pycard^tm1Flv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased susceptibility to induced colitis ( J:173245 )

• mice exhibit increased susceptibility to dextran sodium sulfate (DSS)-induced colitis compared with similarly treated wild-type mice

• mice transmit increased susceptibility to DSS-induced colitis to co-housed or cross-fostered wild-type mice

• mice cross-fostered by wild-type dams exhibit milder colitis than noncross-fostered mice

• however, antibiotic (not oral gancyclovir, amphotericin, or albendazole and praziquantel) treatment restores wild-type levels of susceptibility to DSS-induced colitis

increased IgA level ( J:173245 )

• in untreated mice

increased IgG2c level ( J:173245 )

• in untreated mice

enlarged Peyer's patches ( J:173245 )

• in untreated mice

digestive/alimentary system

abnormal large intestine crypts of Lieberkuhn morphology ( J:173245 )

• colonic crypt hyperplasia in untreated mice

abnormal gut flora balance ( J:173245 )

• mice and co-housed wild-type mice exhibit expanded bacterial phylotypes compared with wild-type mice

increased susceptibility to induced colitis ( J:173245 )

• mice exhibit increased susceptibility to dextran sodium sulfate (DSS)-induced colitis compared with similarly treated wild-type mice

• mice transmit increased susceptibility to DSS-induced colitis to co-housed or cross-fostered wild-type mice

• mice cross-fostered by wild-type dams exhibit milder colitis than noncross-fostered mice

• however, antibiotic (not oral gancyclovir, amphotericin, or albendazole and praziquantel) treatment restores wild-type levels of susceptibility to DSS-induced colitis

endocrine/exocrine glands

abnormal large intestine crypts of Lieberkuhn morphology ( J:173245 )

• colonic crypt hyperplasia in untreated mice

hematopoietic system

increased IgA level ( J:173245 )

• in untreated mice

increased IgG2c level ( J:173245 )

• in untreated mice

Genotype
MGI:3687250

hm2

• Allelic Composition: Pycard^tm1Flv/Pycard^tm1Flv
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6

immune system

decreased NK cell number ( J:158969 )

• mCMV-treated mice exhibit reduced NK cells in the spleen and IFN-gamma producing splenic NK cells compared with similarly treated wild-type mice

abnormal macrophage physiology ( J:113333 )

• impaired secretion of IL1A and IL1B in TLR-primed and ATP-treated cells

decreased circulating interleukin-1 beta level ( J:113333 )

• significant decrease in serum IL1B levels after challenge with 37.5 mg/kg of LPS

decreased circulating interleukin-18 level ( J:113333 , J:158969 )

• significant decrease in serum IL18 levels after challenge with 37.5 mg/kg of LPS (J:113333)

• in mCMV-treated mice (J:158969)

decreased susceptibility to type IV hypersensitivity reaction ( J:113333 )

• no difference in the degree of ear swelling between sensitized (with trinitrophenylchloride) and naive mice

decreased interleukin-1 alpha secretion ( J:113333 )

• impaired secretion of IL1A in TLR-primed and ATP-treated cells

decreased interleukin-1 beta secretion ( J:113333 )

• impaired secretion of IL1B in TLR-primed and ATP-treated cells

decreased susceptibility to endotoxin shock ( J:113333 )

• 24 hours after injection of a lethal dose of LPS (37.5 mg/kg) only 15% of mice have died compared to 90% of wild-type mice and maximal mortality (at 7 days) is only 30%

• delayed cell death response in macrophages infected with Salmonella tymphimurium

increased susceptibility to Herpesvirales infection ( J:158969 )

• mCMV-treated mice exhibit reduced serum IL18 levels, fewer NK cells in the spleen, fewer IFN-gamma-producing splenic NK cells, and increased viral titers compared with similarly treated wild-type mice

homeostasis/metabolism

decreased circulating interleukin-1 beta level ( J:113333 )

• significant decrease in serum IL1B levels after challenge with 37.5 mg/kg of LPS

decreased circulating interleukin-18 level ( J:113333 , J:158969 )

• significant decrease in serum IL18 levels after challenge with 37.5 mg/kg of LPS (J:113333)

• in mCMV-treated mice (J:158969)

hematopoietic system

decreased NK cell number ( J:158969 )

• mCMV-treated mice exhibit reduced NK cells in the spleen and IFN-gamma producing splenic NK cells compared with similarly treated wild-type mice

abnormal macrophage physiology ( J:113333 )

• impaired secretion of IL1A and IL1B in TLR-primed and ATP-treated cells

Genotype
MGI:3850058

cn3

• Allelic Composition: Nlrp3^tm1Hhf/Nlrp3⁺; Pycard^tm1Flv/Pycard^tm1Flv; Lyz2^tm1(cre)Ifo/Lyz2⁺
• Genetic Background: involves: 129 * C57BL/6

normal phenotype

no abnormal phenotype detected ( J:150054 )

• mice appear phenotypically normal and do not develop the inflammatory disease that conditional heterozygotes develop on an intact Pycard background

Genotype
MGI:3722762

cx4

• Allelic Composition: Pycard^tm1Flv/Pycard^tm1Flv; Slc11a1^s/Slc11a1^s
• Genetic Background: B6.129S5-Pycard^tm1Flv

immune system

increased susceptibility to bacterial infection ( J:124378 )

• when infected with Salmonella typhimurium, mice succumb within the same timeframe as Slc11a1^s homozygotes

• when infected with Salmonella typhimurium, spleen and mesenteric lymph nodes bacterial loads are increased similar to in Slc11a1^s homozygotes