Phenotypes associated with this allele

• Allele Symbol: Nlrp3^tm1Flv
• Allele Name: targeted mutation 1, Richard A Flavell
• Allele ID: MGI:3686871
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nlrp3^tm1Flv/Nlrp3^tm1Flv	B6.129S5-Nlrp3^tm1Flv	MGI:4836359
hm2	Nlrp3^tm1Flv/Nlrp3^tm1Flv	involves: 129S5/SvEvBrd	MGI:5085876
hm3	Nlrp3^tm1Flv/Nlrp3^tm1Flv	involves: 129S5/SvEvBrd * C57BL/6	MGI:3687249
cn4	Nlrp3^tm1Flv/Nlrp3^tm1Hhf Lyz2^tm1(cre)Ifo/Lyz2^+	involves: 129 * C57BL/6	MGI:3850059
cx5	Nlrp3^tm1Flv/Nlrp3^tm1Flv Slc11a1^s/Slc11a1^s	B6.129S5-Nlrp3^tm1Flv	MGI:3722761

Genotype
MGI:4836359

hm1

• Allelic Composition: Nlrp3^tm1Flv/Nlrp3^tm1Flv
• Genetic Background: B6.129S5-Nlrp3^tm1Flv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased interleukin-1 beta secretion ( J:164725 )

• by bone marrow derived dendritic cells in response to phenol-purified LPS with ATP and commercial LPS

Genotype
MGI:5085876

hm2

• Allelic Composition: Nlrp3^tm1Flv/Nlrp3^tm1Flv
• Genetic Background: involves: 129S5/SvEvBrd

digestive/alimentary system

decreased susceptibility to induced colitis ( J:173245 )

• dextran sodium sulfate (DSS)-treated mice housed with wild-type mice exhibit attenuated colitis compared with wild-type mice

immune system

decreased susceptibility to induced colitis ( J:173245 )

• dextran sodium sulfate (DSS)-treated mice housed with wild-type mice exhibit attenuated colitis compared with wild-type mice

Genotype
MGI:3687249

hm3

• Allelic Composition: Nlrp3^tm1Flv/Nlrp3^tm1Flv
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6

immune system

abnormal macrophage physiology ( J:113333 )

• do not process caspase-1 and secrete less IL1B in response to LPS and ATP treatment

• impaired secretion of IL1A, IL1B and IL18 in TLR-primed and ATP-treated cells; however secretion of TNFA, IL6, and IL12 are similar to wild-type

• absence of bacterial muramyl dipeptide-stimulated IL1B secretion

decreased circulating interleukin-1 beta level ( J:113333 )

• significant decrease in serum IL1B levels after challenge with 37.5 mg/kg of LPS

decreased circulating interleukin-18 level ( J:113333 )

• significant decrease in serum IL18 levels after challenge with 37.5 mg/kg of LPS

decreased susceptibility to type IV hypersensitivity reaction ( J:113333 )

• no difference in the degree of ear swelling between sensitized (with trinitrophenylchloride) and naive mice

decreased susceptibility to endotoxin shock ( J:113333 )

• with lower doses of LPS (18.75 and 9.38 mg/kg) mortality is delayed or reduced, respectively, compared to wild-type mice

• however with a high dose of LPS ((37.5 mg/kg) no difference in the time course of incidence of mortality is seen and the time course of macrophage cell death in response to Salmonella tymphimurium is similar to wild-type

homeostasis/metabolism

decreased circulating interleukin-1 beta level ( J:113333 )

• significant decrease in serum IL1B levels after challenge with 37.5 mg/kg of LPS

decreased circulating interleukin-18 level ( J:113333 )

• significant decrease in serum IL18 levels after challenge with 37.5 mg/kg of LPS

hematopoietic system

abnormal macrophage physiology ( J:113333 )

• do not process caspase-1 and secrete less IL1B in response to LPS and ATP treatment

• impaired secretion of IL1A, IL1B and IL18 in TLR-primed and ATP-treated cells; however secretion of TNFA, IL6, and IL12 are similar to wild-type

• absence of bacterial muramyl dipeptide-stimulated IL1B secretion

Genotype
MGI:3850059

cn4

• Allelic Composition: Nlrp3^tm1Flv/Nlrp3^tm1Hhf; Lyz2^tm1(cre)Ifo/Lyz2⁺
• Genetic Background: involves: 129 * C57BL/6

mortality/aging

postnatal lethality, complete penetrance ( J:150054 )

• mice die between 2 and 14 days after birth probably due to multisystem organ failure secondary to inflammation and necrosis

• genotype demonstrates inflammatory disease of the conditional allele does not require presence of wild-type allele

immune system

increased inflammatory response ( J:150054 )

• mice have pronounced leukocytic infiltrates in skin, liver, spleen, joint, sinus, conjunctiva, bone marrow, and tongue that are mainly neutrophilic

Genotype
MGI:3722761

cx5

• Allelic Composition: Nlrp3^tm1Flv/Nlrp3^tm1Flv; Slc11a1^s/Slc11a1^s
• Genetic Background: B6.129S5-Nlrp3^tm1Flv

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:124378 )

• when infected with 10⁵ Salmonella typhimurium, mice succumb within the same timeframe as Slc11a1^s homozygotes

immune system

increased susceptibility to bacterial infection ( J:124378 )

• when infected with 10⁵ Salmonella typhimurium, mice succumb within the same timeframe as Slc11a1^s homozygotes

• when infected with 10⁵ Salmonella typhimurium, spleen and mesenteric lymph nodes bacterial loads are increased similar to in Slc11a1^s homozygotes