Phenotypes associated with this allele

• Allele Symbol: Igh^tm11.1Cgn
• Allele Name: targeted mutation 11.1, University of Cologne
• Allele ID: MGI:3687002
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Igh^tm11.1Cgn/Igh^tm11.1Cgn	Not Specified	MGI:3687970
ht2	Igh^deltaD/Igh^tm11.1Cgn	involves: C57BL/6 * DBA/2	MGI:3687971
cx3	Igh-J^tm1Cgn/Igh^tm11.1Cgn	involves: 129P2/OlaHsd	MGI:3687969

Genotype
MGI:3687970

hm1

• Allelic Composition: Igh^tm11.1Cgn/Igh^tm11.1Cgn
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal B cell differentiation ( J:113434 )

• rescue of nonproductive V_H D_H J_H rearrangements and repertoire of IgH chains are performed by V_H replacement or direct V_H to D_H joining

• frequency of joints in mature B cells that have undergone by V_H replacement that contain the extra arginine that would be generated at the junction as well as the arginine encoded at the 3' end of the D23^stop allele is only 12%, indicating that counterselection of complementarity region 3s rich in positively charged amino acids occurs

• V_H replacement and direct V_H to D_H joining compete inefficiently with canonical VDJ recombination in pro-B cell development in 4 week old mice

decreased immature B cell number ( J:113434 )

• there is a reduction in immature (IgM+B220lo) B cells in the bone marrow of young mutants

increased pro-B cell number ( J:113434 )

• a partial block in transition of pro-B cells to pre-B cells is observed; an accumulating corresponding to ~3-fold increase in absolute numbers of pro-B cells occurs

• there is a reduction in immature (IgM+B220lo) and mature (IgM+B220hi) B cells in the bone marrow of young mutants

decreased B cell number ( J:113434 )

• mice generate reduced B cell numbers (~5 million/spleen) compared to wild-type (25 million/spleen)

• one third are typical mature follicular B cells and there is substantial accumulation of marginal zone B cells

decreased mature B cell number ( J:113434 )

• there is a reduction in mature (IgM+B220hi) B cells in the bone marrow of young mutants

hematopoietic system

abnormal B cell differentiation ( J:113434 )

• rescue of nonproductive V_H D_H J_H rearrangements and repertoire of IgH chains are performed by V_H replacement or direct V_H to D_H joining

• frequency of joints in mature B cells that have undergone by V_H replacement that contain the extra arginine that would be generated at the junction as well as the arginine encoded at the 3' end of the D23^stop allele is only 12%, indicating that counterselection of complementarity region 3s rich in positively charged amino acids occurs

• V_H replacement and direct V_H to D_H joining compete inefficiently with canonical VDJ recombination in pro-B cell development in 4 week old mice

decreased immature B cell number ( J:113434 )

• there is a reduction in immature (IgM+B220lo) B cells in the bone marrow of young mutants

increased pro-B cell number ( J:113434 )

• a partial block in transition of pro-B cells to pre-B cells is observed; an accumulating corresponding to ~3-fold increase in absolute numbers of pro-B cells occurs

• there is a reduction in immature (IgM+B220lo) and mature (IgM+B220hi) B cells in the bone marrow of young mutants

decreased B cell number ( J:113434 )

• mice generate reduced B cell numbers (~5 million/spleen) compared to wild-type (25 million/spleen)

• one third are typical mature follicular B cells and there is substantial accumulation of marginal zone B cells

decreased mature B cell number ( J:113434 )

• there is a reduction in mature (IgM+B220hi) B cells in the bone marrow of young mutants

Genotype
MGI:3687971

ht2

• Allelic Composition: Igh^deltaD/Igh^tm11.1Cgn
• Genetic Background: involves: C57BL/6 * DBA/2

immune system

abnormal B cell differentiation ( J:113434 )

• analysis shows that V_H replacement contributes more or is more frequent direct V_H to D_H joining in determining IgH chain repertoire

hematopoietic system

abnormal B cell differentiation ( J:113434 )

• analysis shows that V_H replacement contributes more or is more frequent direct V_H to D_H joining in determining IgH chain repertoire

Genotype
MGI:3687969

cx3

• Allelic Composition: Igh-J^tm1Cgn/Igh^tm11.1Cgn
• Genetic Background: involves: 129P2/OlaHsd

immune system

abnormal B cell differentiation ( J:113434 )

• frequency of joints in mature B cells that have undergone by V_H replacement that contain the extra arginine that would be generated at the junction as well as the arginine encoded at the 3' end of the D23^stop allele is only 12%, indicating that counterselection of complementarity region 3s rich in positively charged amino acids occurs

• V_H replacement and direct V_H to D_H joining compete inefficiently with canonical VDJ recombination in B cell development in vivo in 2.5 month old mice

hematopoietic system

abnormal B cell differentiation ( J:113434 )

• frequency of joints in mature B cells that have undergone by V_H replacement that contain the extra arginine that would be generated at the junction as well as the arginine encoded at the 3' end of the D23^stop allele is only 12%, indicating that counterselection of complementarity region 3s rich in positively charged amino acids occurs

• V_H replacement and direct V_H to D_H joining compete inefficiently with canonical VDJ recombination in B cell development in vivo in 2.5 month old mice