Phenotypes associated with this allele

• Allele Symbol: Ucp2^{Gt(S20-3G1)Sor}
• Allele Name: gene trap S20-3G1, Philippe Soriano
• Allele ID: MGI:3687318
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ucp2^Gt(S20-3G1)Sor/Ucp2^Gt(S20-3G1)Sor	involves: CD-1	MGI:3689929
hm2	Ucp2^Gt(S20-3G1)Sor/Ucp2^Gt(S20-3G1)Sor	Not Specified	MGI:3811499
cx3	Tg(Npy-MAPT/Sapphire)1Rck/? Ucp2^Gt(S20-3G1)Sor/Ucp2^Gt(S20-3G1)Sor	involves: C57BL/6 * CBA	MGI:3811500
cx4	Tg(Pomc-MAPT/Topaz)1Rck/0 Ucp2^Gt(S20-3G1)Sor/Ucp2^Gt(S20-3G1)Sor	involves: C57BL/6 * CBA	MGI:3811501

Genotype
MGI:3689929

hm1

• Allelic Composition: Ucp2^{Gt(S20-3G1)Sor}/Ucp2^{Gt(S20-3G1)Sor}
• Genetic Background: involves: CD-1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

impaired righting response ( J:113539 )

• after initial i.p. injection of 3.5 mg/kg ethanol, mutants show longer latencies in regaining righting reflex compared to controls

abnormal locomotor behavior ( J:113539 )

• mice show a longer latency to resume locomotion after first i.p. injection of ethanol compared to wild-type

increased thermal nociceptive threshold ( J:113539 )

• prior to alcohol treatment on first and fifth days of testing , mutant withdraw from hot plate at similar pace to controls; 4 hours after injection, mutants move away from hot plate more slowly than controls on both the first and fifth test day

Genotype
MGI:3811499

hm2

• Allelic Composition: Ucp2^{Gt(S20-3G1)Sor}/Ucp2^{Gt(S20-3G1)Sor}
• Genetic Background: Not Specified

behavior/neurological

abnormal food intake ( J:139938 )

• hyperphagia that normally occurs from ghrelin treatment is severely attenuated in these mice

• food intake was about half that of controls both 1 and 2 hours after ghrelin treatment

• this attenuation in ghrelin-induced food intake still occurred if ghrelin was administered directly into the hypothalamus

nervous system

abnormal hypothalamus morphology ( J:139938 )

• hypothalamus cells fail to increase their number of mitochondria in response to ghrelin treatment where as in wild-type mitochondrial numbers are significantly increased

abnormal hypothalamus physiology ( J:139938 )

• uncoupled oxygen consumption by hypothalamic mitochondria is increased in wild-type mice treated with ghrelin hormone but not in these mutant mice

• total respiratory capacity of these mitochondria also fail to increase in mice treated with ghrelin hormone compared to wild-type which have about a 50% increase in total respiratory capacity

• the membrane potential of mitochondria fail to be lowered by ghrelin treatment as in controls

• hypothalamic mitochondria significantly increase their production of reactive oxygen species in response to ghrelin-treatment unlike in wild-type mice where increases in ROS production are limited

Genotype
MGI:3811500

cx3

• Allelic Composition: Tg(Npy-MAPT/Sapphire)1Rck/?; Ucp2^{Gt(S20-3G1)Sor}/Ucp2^{Gt(S20-3G1)Sor}
• Genetic Background: involves: C57BL/6 * CBA

nervous system

abnormal hypothalamus physiology ( J:139938 )

• ghrelin increases mean action potential frequency of GFP-labeled neuronal perikarya in whole-cell recordings of hypothalamic coronal slices from wild-type mice but not in these mice

Genotype
MGI:3811501

cx4

• Allelic Composition: Tg(Pomc-MAPT/Topaz)1Rck/0; Ucp2^{Gt(S20-3G1)Sor}/Ucp2^{Gt(S20-3G1)Sor}
• Genetic Background: involves: C57BL/6 * CBA

nervous system

abnormal hypothalamus physiology ( J:139938 )

• ghrelin induced recruitment of miniature inhibitory postsynaptic currents on GFP-labeled cells within the hypothalamus was significantly elevated in wild-type animals but not in these mice

abnormal synaptic plasticity ( J:139938 )

• grehlin increases the number of symmetric (inhibitory) synapses and decreases the number of asymmetric (excitatory) synapases found in hypothalamus in wild-type but not mutant mice