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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Il5tm1Anjm
targeted mutation 1, Andrew NJ McKenzie
MGI:3688245
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Il5tm1Anjm/Il5tm1Anjm involves: 129P2/OlaHsd * C57BL/6 MGI:3689873
cx2
 		Il5tm1Anjm/Il5tm1Anjm
Il9tm1Anjm/Il9tm1Anjm 		involves: 129P2/OlaHsd * C57BL/6 MGI:3689874


Genotype
MGI:3689873
hm1
Allelic
Composition		 Il5tm1Anjm/Il5tm1Anjm
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il5tm1Anjm mutation (0 available); any Il5 mutation (31 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased eosinophil cell number ( J:113543 )
• with absence of Il4 and Il5, eosinophilia is completely blocked
abnormal mast cell morphology ( J:113543 )
• in response to infection, mutants still generate mast cell hyperplasia (mast cell proliferation) equivalent to wild-type
abnormal mast cell physiology ( J:113543 )
• mutants produce higher levels of mast cell protease-1 compared to wild-type in response to parasitic infection
decreased IgE level ( J:113543 )
• mutants fail to produce IgE, due to deletion of Il4
granulomatous inflammation ( J:113543 )
• primary granuloma formation in and volumes in lungs after infection are severely reduced (<10%) compared to wild-type
• secondary granuloma formation is essentially completely blocked
abnormal susceptibility to infection ( J:113543 )
• expulsion of worms from intestines (50% by day 35) is delayed compared to wild-type
increased susceptibility to parasitic infection ( J:113543 )
• after N. brasiliensis (nematode) infection, expulsion of worms from intestines is delayed with ~50% of worms remaining in intestine after 25 days, whereas wild-type mice expel all worms within 5-10 days

digestive/alimentary system
abnormal intestinal goblet cell morphology ( J:113543 )
• goblet cell hyperplasia (increased number) is significantly delayed vs wild-type, and numbers do not increase until 30 days post-infection

hematopoietic system
decreased eosinophil cell number ( J:113543 )
• with absence of Il4 and Il5, eosinophilia is completely blocked
abnormal mast cell morphology ( J:113543 )
• in response to infection, mutants still generate mast cell hyperplasia (mast cell proliferation) equivalent to wild-type
abnormal mast cell physiology ( J:113543 )
• mutants produce higher levels of mast cell protease-1 compared to wild-type in response to parasitic infection
decreased IgE level ( J:113543 )
• mutants fail to produce IgE, due to deletion of Il4

cellular
abnormal intestinal goblet cell morphology ( J:113543 )
• goblet cell hyperplasia (increased number) is significantly delayed vs wild-type, and numbers do not increase until 30 days post-infection




Genotype
MGI:3689874
cx2
Allelic
Composition		 Il5tm1Anjm/Il5tm1Anjm
Il9tm1Anjm/Il9tm1Anjm
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il5tm1Anjm mutation (0 available); any Il5 mutation (31 available)
Il9tm1Anjm mutation (2 available); any Il9 mutation (22 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased eosinophil cell number ( J:113543 )
• with absence of Il4 and Il5, eosinophilia is completely blocked
abnormal mast cell morphology ( J:113543 )
• mastocytosis is abolished compared to wild-type or triple mutants
abnormal mast cell physiology ( J:113543 )
• no jejunal mast cell protease 1 is produced by mutants compared to wild-type or other allele combinations
abnormal interleukin level ( J:113543 )
• Il-10 expression is reduced compared to Il5tm1Anjm mutants
abnormal humoral immune response ( J:113543 )
• mice show a switch to a Th1 response and Il12 production from a Th2 response
decreased IgE level ( J:113543 )
• mutants fail to produce IgE, due to deletion of Il4
granulomatous inflammation ( J:113543 )
• primary granuloma formation and volumes in lungs after infection are severely reduced (<10%) compared to wild-type
• secondary granuloma formation is essentially blocked
abnormal susceptibility to infection ( J:113543 )
• expulsion of worms is delayed by an order of magnitude vs triple mutants
increased susceptibility to parasitic infection ( J:113543 )
• compound quadruple mutants show ~1 order of magnitude greater delay in expulsion of worms from intestine (50% remain at day 60 after infection) than triple mutants (35 days)
• more worms remain at 40 days post infection than in triple Il4/Il5/Il13 mutants or wild-type

digestive/alimentary system
abnormal intestinal goblet cell morphology ( J:113543 )
• goblet cell hyperplasia is severely impaired after nematode infection with little increase in cell number observed even at 40 days post-infection

hematopoietic system
decreased eosinophil cell number ( J:113543 )
• with absence of Il4 and Il5, eosinophilia is completely blocked
abnormal mast cell morphology ( J:113543 )
• mastocytosis is abolished compared to wild-type or triple mutants
abnormal mast cell physiology ( J:113543 )
• no jejunal mast cell protease 1 is produced by mutants compared to wild-type or other allele combinations
decreased IgE level ( J:113543 )
• mutants fail to produce IgE, due to deletion of Il4

homeostasis/metabolism
abnormal interleukin level ( J:113543 )
• Il-10 expression is reduced compared to Il5tm1Anjm mutants

cellular
abnormal intestinal goblet cell morphology ( J:113543 )
• goblet cell hyperplasia is severely impaired after nematode infection with little increase in cell number observed even at 40 days post-infection




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
10/29/2024
MGI 6.24 		The Jackson Laboratory