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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tg(Tcra2B4)1Mmd
transgene insertion 1, Mark M Davis
MGI:3688270
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
 		Itktm1Ljb/Itktm1Ljb
Tg(Tcra2B4)1Mmd/?
Tg(Tcrb2B4)1Mmd/? 		involves: C3H/HeJ * C57BL/6 * C57BL/10 MGI:4354594
cx2
 		Tg(H2KmPCC)2939Stoe/0
Tg(Tcra2B4)1Mmd/0
Tg(Tcrb2B4)1Mmd/0 		involves: C3H/HeJ * C57BL/6 * SJL MGI:3688409
cx3
 		Dock2tm1Tsas/Dock2tm1Tsas
Tg(Tcra2B4)1Mmd/?
Tg(Tcrb2B4)1Mmd/? 		involves: C57BL/6 MGI:3690240
tg4
 		Tg(Tcra2B4)1Mmd/? involves: C3H/HeJ * C57BL/6 MGI:3835053


Genotype
MGI:4354594
cx1
Allelic
Composition		 Itktm1Ljb/Itktm1Ljb
Tg(Tcra2B4)1Mmd/?
Tg(Tcrb2B4)1Mmd/?
Genetic
Background		 involves: C3H/HeJ * C57BL/6 * C57BL/10
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itktm1Ljb mutation (0 available); any Itk mutation (50 available)
Tg(Tcra2B4)1Mmd mutation (0 available)
Tg(Tcrb2B4)1Mmd mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased CD4-positive, alpha-beta T cell number ( J:123795 )
• the percentages and absolute numbers of CD4+ single positive thymocytes is drastically reduced compared to wild-type and transgenic controls
• peripheral CD4 T cell numbers are also reduced

hematopoietic system
decreased CD4-positive, alpha-beta T cell number ( J:123795 )
• the percentages and absolute numbers of CD4+ single positive thymocytes is drastically reduced compared to wild-type and transgenic controls
• peripheral CD4 T cell numbers are also reduced




Genotype
MGI:3688409
cx2
Allelic
Composition		 Tg(H2KmPCC)2939Stoe/0
Tg(Tcra2B4)1Mmd/0
Tg(Tcrb2B4)1Mmd/0
Genetic
Background		 involves: C3H/HeJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(H2KmPCC)2939Stoe mutation (1 available)
Tg(Tcra2B4)1Mmd mutation (0 available)
Tg(Tcrb2B4)1Mmd mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased T cell proliferation ( J:50143 )
• mice have no in vitro response to cytochrome C while spleen cells from Tcra-knockin, transgenic Tcrb-expressing mice exhibit a vigorous proliferation response
decreased thymocyte number ( J:50143 )
• number of thymocytes (2-4 x 106) in tolerant mice is reduced 30 to 40-fold compared with normal TCR transgenic mice (Tg(H2KmPCC)2939Stoe-negative, TcraTcrb-positive mice; 70-90 x 106 cells)
abnormal T cell differentiation ( J:50143 )
• number of thymocytes is drastically reduced and few thymocytes undergo natural development using the endogenous Tcra/transgenic Tcrb chain compared to Tcra-knockin, transgenic Tcrb-expressing mice

hematopoietic system
decreased T cell proliferation ( J:50143 )
• mice have no in vitro response to cytochrome C while spleen cells from Tcra-knockin, transgenic Tcrb-expressing mice exhibit a vigorous proliferation response
decreased thymocyte number ( J:50143 )
• number of thymocytes (2-4 x 106) in tolerant mice is reduced 30 to 40-fold compared with normal TCR transgenic mice (Tg(H2KmPCC)2939Stoe-negative, TcraTcrb-positive mice; 70-90 x 106 cells)
abnormal T cell differentiation ( J:50143 )
• number of thymocytes is drastically reduced and few thymocytes undergo natural development using the endogenous Tcra/transgenic Tcrb chain compared to Tcra-knockin, transgenic Tcrb-expressing mice

endocrine/exocrine glands
decreased thymocyte number ( J:50143 )
• number of thymocytes (2-4 x 106) in tolerant mice is reduced 30 to 40-fold compared with normal TCR transgenic mice (Tg(H2KmPCC)2939Stoe-negative, TcraTcrb-positive mice; 70-90 x 106 cells)

cellular
decreased T cell proliferation ( J:50143 )
• mice have no in vitro response to cytochrome C while spleen cells from Tcra-knockin, transgenic Tcrb-expressing mice exhibit a vigorous proliferation response




Genotype
MGI:3690240
cx3
Allelic
Composition		 Dock2tm1Tsas/Dock2tm1Tsas
Tg(Tcra2B4)1Mmd/?
Tg(Tcrb2B4)1Mmd/?
Genetic
Background		 involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dock2tm1Tsas mutation (1 available); any Dock2 mutation (143 available)
Tg(Tcra2B4)1Mmd mutation (0 available)
Tg(Tcrb2B4)1Mmd mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased double-positive T cell number ( J:84581 )
• numbers reduced to about 3% of what is seen in controls
• more susceptible to apoptosis
abnormal CD4-positive, alpha beta T cell morphology ( J:84581 )
• reduced in number and ratio in relation to CD8+ T cells is also reduced
abnormal T cell physiology ( J:84581 )
• proliferative responses are reduced

hematopoietic system
decreased double-positive T cell number ( J:84581 )
• numbers reduced to about 3% of what is seen in controls
• more susceptible to apoptosis
abnormal CD4-positive, alpha beta T cell morphology ( J:84581 )
• reduced in number and ratio in relation to CD8+ T cells is also reduced
abnormal T cell physiology ( J:84581 )
• proliferative responses are reduced




Genotype
MGI:3835053
tg4
Allelic
Composition		 Tg(Tcra2B4)1Mmd/?
Genetic
Background		 involves: C3H/HeJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal thymus cell ratio ( J:92992 )
• the ratio of double negative to double-positive thymocytes is significantly higher than in controls
decreased thymocyte number ( J:92992 )
• thymus cellularity is reduced by about 4 fold compared to controls
increased double-negative T cell number ( J:92992 )
• an increase in double-negative thymocytes is noted in these mice
decreased double-positive T cell number ( J:92992 )
• a decrease in double-negative thymocytes is noted in these mice
decreased CD4-positive, alpha-beta T cell number ( J:92992 )
• the absolute number of single-positive CD4 thymocytes is decreased

neoplasm
increased lymphoma incidence ( J:92992 )
• 19.4% of mice develop lymphoma by 6 months of age

hematopoietic system
abnormal thymus cell ratio ( J:92992 )
• the ratio of double negative to double-positive thymocytes is significantly higher than in controls
decreased thymocyte number ( J:92992 )
• thymus cellularity is reduced by about 4 fold compared to controls
increased double-negative T cell number ( J:92992 )
• an increase in double-negative thymocytes is noted in these mice
decreased double-positive T cell number ( J:92992 )
• a decrease in double-negative thymocytes is noted in these mice
decreased CD4-positive, alpha-beta T cell number ( J:92992 )
• the absolute number of single-positive CD4 thymocytes is decreased

endocrine/exocrine glands
abnormal thymus cell ratio ( J:92992 )
• the ratio of double negative to double-positive thymocytes is significantly higher than in controls
decreased thymocyte number ( J:92992 )
• thymus cellularity is reduced by about 4 fold compared to controls




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory