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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Lcktm1Dbs
targeted mutation 1, David B Straus
MGI:3690319
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Lcktm1Dbs/Lcktm1Dbs involves: C3H * C57BL/6 MGI:3698306
cx2
 		Lcktm1Dbs/Lcktm1Dbs
Tg(TcraTcrb)1100Mjb/0 		involves: C3H * C57BL/6 MGI:3698307
cx3
 		Lcktm1Dbs/Lcktm1Dbs
Tg(TcraTcrb)425Cbn/0 		involves: C3H * C57BL/6 MGI:3698308
cx4
 		Lcktm1Dbs/Lcktm1Dbs
Tg(TcraH-Y,TcrbH-Y)71Vbo/0 		involves: C3H * C57BL/6 * DBA/2J MGI:3698309


Genotype
MGI:3698306
hm1
Allelic
Composition		 Lcktm1Dbs/Lcktm1Dbs
Genetic
Background		 involves: C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lcktm1Dbs mutation (0 available); any Lck mutation (95 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
decreased T cell proliferation ( J:114660 )
• mutant thymocytes show defective proliferation (>5-fold reduction) following receptor engagement in vitro
• induction of CD69 expression after TCR stimulation is reduced
abnormal T cell differentiation ( J:114660 )
• T cell development is altered as mutants have ~50% of thymocyte number of wild-type
abnormal double-negative T cell morphology ( J:114660 )
• relative accumulation of CD25+CD44- thymocytes (double negative stage 3, DN3) compared to wild-type
• DN3/DN4 ratio is 10 in mutants vs ~2 in controls
decreased T cell number ( J:114660 )
• reduced numbers of T cells in lymph nodes and spleen
decreased thymocyte number ( J:114660 )
• mutants have ~50% of thymocyte number of wild-type
abnormal CD4-positive, alpha beta T cell morphology ( J:114660 )
• numbers of mature thymocytes are 2-3-fold less than in controls
• however, DN thymocyte numbers are relatively normal
abnormal CD8-positive, alpha beta T cell morphology ( J:114660 )
• numbers of mature thymocytes are 2-3-fold less than in controls
• however, DN thymocyte numbers are relatively normal

immune system
decreased T cell proliferation ( J:114660 )
• mutant thymocytes show defective proliferation (>5-fold reduction) following receptor engagement in vitro
• induction of CD69 expression after TCR stimulation is reduced
abnormal T cell differentiation ( J:114660 )
• T cell development is altered as mutants have ~50% of thymocyte number of wild-type
abnormal double-negative T cell morphology ( J:114660 )
• relative accumulation of CD25+CD44- thymocytes (double negative stage 3, DN3) compared to wild-type
• DN3/DN4 ratio is 10 in mutants vs ~2 in controls
decreased T cell number ( J:114660 )
• reduced numbers of T cells in lymph nodes and spleen
decreased thymocyte number ( J:114660 )
• mutants have ~50% of thymocyte number of wild-type
abnormal CD4-positive, alpha beta T cell morphology ( J:114660 )
• numbers of mature thymocytes are 2-3-fold less than in controls
• however, DN thymocyte numbers are relatively normal
abnormal CD8-positive, alpha beta T cell morphology ( J:114660 )
• numbers of mature thymocytes are 2-3-fold less than in controls
• however, DN thymocyte numbers are relatively normal

endocrine/exocrine glands
decreased thymocyte number ( J:114660 )
• mutants have ~50% of thymocyte number of wild-type

cellular
decreased T cell proliferation ( J:114660 )
• mutant thymocytes show defective proliferation (>5-fold reduction) following receptor engagement in vitro
• induction of CD69 expression after TCR stimulation is reduced




Genotype
MGI:3698307
cx2
Allelic
Composition		 Lcktm1Dbs/Lcktm1Dbs
Tg(TcraTcrb)1100Mjb/0
Genetic
Background		 involves: C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lcktm1Dbs mutation (0 available); any Lck mutation (95 available)
Tg(TcraTcrb)1100Mjb mutation (6 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
decreased T cell proliferation ( J:114660 )
• transgenic CD8+ thymocytes are not fully mature
abnormal positive T cell selection ( J:114660 )
• positive selection of CD4 and CD8 lineage is diminished

immune system
decreased T cell proliferation ( J:114660 )
• transgenic CD8+ thymocytes are not fully mature
abnormal positive T cell selection ( J:114660 )
• positive selection of CD4 and CD8 lineage is diminished

cellular
decreased T cell proliferation ( J:114660 )
• transgenic CD8+ thymocytes are not fully mature




Genotype
MGI:3698308
cx3
Allelic
Composition		 Lcktm1Dbs/Lcktm1Dbs
Tg(TcraTcrb)425Cbn/0
Genetic
Background		 involves: C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lcktm1Dbs mutation (0 available); any Lck mutation (95 available)
Tg(TcraTcrb)425Cbn mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal positive T cell selection ( J:114660 )
• positive selection of CD4 and CD8 lineage is compromised
arrested T cell differentiation ( J:114660 )
• almost complete block of positive selection is observed

immune system
abnormal positive T cell selection ( J:114660 )
• positive selection of CD4 and CD8 lineage is compromised
arrested T cell differentiation ( J:114660 )
• almost complete block of positive selection is observed




Genotype
MGI:3698309
cx4
Allelic
Composition		 Lcktm1Dbs/Lcktm1Dbs
Tg(TcraH-Y,TcrbH-Y)71Vbo/0
Genetic
Background		 involves: C3H * C57BL/6 * DBA/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lcktm1Dbs mutation (0 available); any Lck mutation (95 available)
Tg(TcraH-Y,TcrbH-Y)71Vbo mutation (4 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal positive T cell selection ( J:114660 )
• almost complete block of positive selection is observed
arrested T cell differentiation ( J:114660 )
• almost complete block of positive selection is observed

immune system
abnormal positive T cell selection ( J:114660 )
• almost complete block of positive selection is observed
arrested T cell differentiation ( J:114660 )
• almost complete block of positive selection is observed




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
12/17/2024
MGI 6.24 		The Jackson Laboratory