Phenotypes associated with this allele

• Allele Symbol: Tg(Pitx1-cre)7Rsd
• Allele Name: transgene insertion 7, Michael G Rosenfeld
• Allele ID: MGI:3690467
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ctnnb1^tm2Kem/Ctnnb1^tm2Kem Tg(Pitx1-cre)7Rsd/0	involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6	MGI:3691353
cn2	Ctnnb1^tm1Mmt/Ctnnb1^tm1Mmt Tg(Pitx1-cre)7Rsd/0	involves: 129X1/SvJ * BALB/c * C57BL/6	MGI:3691354
cn3	Kdm1a^tm1.1Rsd/Kdm1a^tm1.1Rsd Tg(Pitx1-cre)7Rsd/0	involves: BALB/c * C57BL/6	MGI:3711646

Genotype
MGI:3691353

cn1

• Allelic Composition: Ctnnb1^tm2Kem/Ctnnb1^tm2Kem; Tg(Pitx1-cre)7Rsd/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

abnormal adenohypophysis morphology ( J:114817 )

• markers for somatropes, thymotropes, and lactotropes are absent in E17.5-P0 mutant pituitary glands; gonadotropes are reduced in number and cells of corticotrope/melanotrope lineage are increased

increased corticotroph cell number ( J:114817 )

decreased gonadotroph cell number ( J:114817 )

absent lactotrophs ( J:114817 )

absent somatotrophs ( J:114817 )

absent thyrotrophs ( J:114817 )

small adenohypophysis ( J:114817 )

• in early conditional knockout for Ctnnb1, at E17.5-P0, anterior pituitary gland is smaller than wild-type and displays caudal expansion of the lumen

nervous system

abnormal adenohypophysis morphology ( J:114817 )

• markers for somatropes, thymotropes, and lactotropes are absent in E17.5-P0 mutant pituitary glands; gonadotropes are reduced in number and cells of corticotrope/melanotrope lineage are increased

increased corticotroph cell number ( J:114817 )

decreased gonadotroph cell number ( J:114817 )

absent lactotrophs ( J:114817 )

absent somatotrophs ( J:114817 )

absent thyrotrophs ( J:114817 )

small adenohypophysis ( J:114817 )

• in early conditional knockout for Ctnnb1, at E17.5-P0, anterior pituitary gland is smaller than wild-type and displays caudal expansion of the lumen

Genotype
MGI:3691354

cn2

• Allelic Composition: Ctnnb1^tm1Mmt/Ctnnb1^tm1Mmt; Tg(Pitx1-cre)7Rsd/0
• Genetic Background: involves: 129X1/SvJ * BALB/c * C57BL/6

endocrine/exocrine glands

absent pituitary gland ( J:114817 )

• with early gain-of-function mutation, at E9.5, initial organ commitment is normal, but by E13.5, there is complete absence of pituitary gland in all mutants

nervous system

absent pituitary gland ( J:114817 )

• with early gain-of-function mutation, at E9.5, initial organ commitment is normal, but by E13.5, there is complete absence of pituitary gland in all mutants

Genotype
MGI:3711646

cn3

• Allelic Composition: Kdm1a^tm1.1Rsd/Kdm1a^tm1.1Rsd; Tg(Pitx1-cre)7Rsd/0
• Genetic Background: involves: BALB/c * C57BL/6

endocrine/exocrine glands

abnormal pituitary gland development ( J:121427 )

• beginning on E13.5, pituitary glands have increased numbers of luminal cells in S phase relative to controls

• at E17.5, glands show a convoluted lumen and exhibit higher proliferation of luminal cells

nervous system

nervous system phenotype ( J:121427 )

N

abnormal pituitary gland development ( J:121427 )

• beginning on E13.5, pituitary glands have increased numbers of luminal cells in S phase relative to controls

• at E17.5, glands show a convoluted lumen and exhibit higher proliferation of luminal cells

homeostasis/metabolism

abnormal pituitary hormone level ( J:121427 )

• few pro-opiomelanocortin-positive cells (POMCs) are detected in the pituitary gland, resulting in decreased protein levels

decreased growth hormone level ( J:121427 )

• protein levels are barely detectable relative to wild-type

abnormal luteinizing hormone level ( J:121427 )

• few LH-positive cells are detected in the pituitary gland resulting in decreased protein levels in pituitary

decreased thyroid-stimulating hormone level ( J:121427 )

• protein levels are barely detectable relative to wild-type

cellular

abnormal cell cycle ( J:121427 )

• more cells in lumen are in S phase at ~E13.5 relative to controls

• some cell cycle regulation genes are upregulated in mutants at E17.5