Analysis Tools
cardiovascular system
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• maximum rate of left ventricular pressure rise and fall (dP/dtmax and dP/dtmin, respectively) recover to 77 and 67% of baseline compared to 11 and 11% of baseline in wild-type after 20 minutes of ischemia and 120 minutes of reperfusion
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• after 20 minutes of ischemia, followed by 120 minutes of reperfusion, mutant hearts attain 73% of baseline LVDP value, compared to 15% of baseline in wild-type hearts
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• upon ischemia/reperfusion, over 50% of the myocardium becomes infarcted in wild-type, compared to only about 25% in mutant hearts
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cellular
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• cultured thymocytes treated with dexamethazsone are refractory to apoptosis compared to wild-type or Trp53-deficient cells but undergo cell death by 46 hours
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• cultured thymocytes maintain viability after gamma-irradiation
• P5 mice exposed to 18 Gy gamma-irradiation show almost complete abrogation of apoptosis when examined after 6 hour recovery
• cell death throughout developing nervous system (EGL, dentate gyrus, retina and subventricular zone of lateral ventricle) of mutants is nearly absent compared to high levels in controls after gamma-irradiation
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• apoptosis after ischemia/reperfusion is ~0.3%, compared to ~1% in wild-type, whereas normoxic control hearts show about 0.1% apoptosis
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nervous system
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• cell death throughout developing nervous system (EGL, dentate gyrus, retina and subventricular zone of lateral ventricle) of mutants is nearly absent compared to high levels in controls after gamma-irradiation
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homeostasis/metabolism
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• upon ischemia/reperfusion, over 50% of the myocardium becomes infarcted in wild-type, compared to only about 25% in mutant hearts
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• after ischemia/reperfusion, LDH release into cardiac effluent is reduced ~50% compared to controls
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hematopoietic system
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• unlike in Bnip3ltm1Ney homozygotes, reticulocytes clearance of mitochondria is normal
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