Phenotypes associated with this allele

• Allele Symbol: Grin1^tm1Yql
• Allele Name: targeted mutation 1, Yuqing Li
• Allele ID: MGI:3692441
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Grin1^tm1Yql/Grin1^tm1Yql Rgs9^tm1.1(cre)Yql/Rgs9^+	involves: 129/Sv * BALB/c * C57BL/6	MGI:3693305
cn2	Grin1^tm1Yql/Grin1^tm1Yql Tg(Ppp1r2-cre)4127Nkza/0	involves: C57BL/6N	MGI:4421576

Genotype
MGI:3693305

cn1

• Allelic Composition: Grin1^tm1Yql/Grin1^tm1Yql; Rgs9^{tm1.1(cre)Yql}/Rgs9⁺
• Genetic Background: involves: 129/Sv * BALB/c * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal motor learning ( J:115299 )

• in rotarod tests, mutants do not show increased duration on rotating rod with successive trials, but control mice do; performance by wild-type on final 3 trials is significantly better than mutants which show impaired motor learning

nervous system

abnormal medium spiny neuron morphology ( J:115299 )

• medium spiny neurons have higher primary dendrite numbers (8.2) compared to wild-type (7)

abnormal excitatory postsynaptic currents ( J:115299 )

• NMDA-receptor mediated EPSCs are nearly abolished in mutants compared to wild-type

abnormal NMDA-mediated synaptic currents ( J:115299 )

• density of current activated by NMDA is reduced greatly in mutants vs controls

reduced long-term potentiation ( J:115299 )

• after high frequency stimulation, dorsomedial striatal slices from mutants do not display LTP

absent long-term depression ( J:115299 )

• brain slices that include the nucleus accumbens (NAc) from mutant mice do not show LTD with moderate-frequency stimulation of the NAc

Genotype
MGI:4421576

cn2

• Allelic Composition: Grin1^tm1Yql/Grin1^tm1Yql; Tg(Ppp1r2-cre)4127Nkza/0
• Genetic Background: involves: C57BL/6N

growth/size/body

growth/size/body region phenotype ( J:156785 )

N • male mice (8-18 weeks) single-housed for 1 week or longer show no abnormalities in growth rate

behavior/neurological

behavior/neurological phenotype ( J:156785 )

N • male mice (8-18 weeks) single-housed for 1 week or longer show no impairment in pain, sensitivity or motor coordination

N • no locomotor abnormality is apparent at 9-10 weeks of age

N • single-housed mice perform normally in a forced swim test at 12-15 weeks, displaying no depressive-like behavior

N • spatial acuity is normal

abnormal spatial working memory ( J:156785 )

• in a Y-maze test, mutants show a reduction in alternatation down to chance levels relative to controls which demonstrate reliable alternation

• administration of risperidone for 3 weeks alleviate the observed deficit

abnormal liquid preference ( J:156785 )

• in a 2 bottle saccharine test, mice receiving 8 weeks of social isolation starting at 8 weeks of age show a decreased preference for sweet solutions, but total liquid intake is unchanged compared to controls

increased anxiety-related response ( J:156785 )

• in an elevated plus maze test, group-housed mice display anxiety-like behavior at 16 weeks of age; single-housed animals show social isolation-induced stress and show anxiety-like behavior earlier, at 8 weeks of age

increased thigmotaxis ( J:156785 )

• animals spend significantly less time exploring the center area of an open field

increased locomotor activity ( J:156785 )

• novelty-induced hyperlocomotion is prominent during the first 3 minutes of spontaneous exploration in open-field test

abnormal nest building behavior ( J:156785 )

• mutants older than 12 weeks display definite deficit in nest-building behavior; mice that were group-housed until 16 weeks of age show a milder nest-building deficit

abnormal sexual interaction ( J:156785 )

♂ • breeding efficiency is significantly decreased in males older than 12 weeks that had been socially isolated from 7 weeks of age

abnormal social investigation ( J:156785 )

• when presented with a 4-week old stimulus male, 12-week old mutants do not investigate the stimulus mouse during the initial 1-minute presentation, which may indicate enhanced anxiety

• increased social investigation toward the novel animal is exhibited after the second trial (presentation) and this is maintained in subsequent trials without habituation, in contrast to control mice which show decreased interest in the stimulus animal in subsequent trials

nervous system

abnormal nervous system electrophysiology ( J:156785 )

• putative pyramidal neurons from 13-14 week old mice show increased mean firing rates and decreased cross-correlation with nearby neurons compared to controls

decreased prepulse inhibition ( J:156785 )

• 10-12 week old mice that were single-housed show an impaired prepulse inhibition of the auditory startle reflex, although the reflex amplitude is normal; a similar deficit is observed in age-matched group-housed mutants

• defects are partially ameliorated by risperidone

hearing/vestibular/ear

hearing/vestibular/ear phenotype ( J:156785 )

N • male mice (8-18 weeks) single-housed for 1 week or longer show no impairment in auditory function

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:156785 )

N • male mice (8-18 weeks) single-housed for 1 week or longer show no impairment in thermoregulation

vision/eye

vision/eye phenotype ( J:156785 )

N • male mice (8-18 weeks) single-housed for 1 week or longer show no impairment in visual function