Phenotypes associated with this allele

• Allele Symbol: Rgs9^{tm1.1(cre)Yql}
• Allele Name: targeted mutation 1.1, Yuqing Li
• Allele ID: MGI:3692442
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Rgs9^tm1.1(cre)Yql/Rgs9^+ Tg(CAG-Ppard*E411P)#Als/0	B6J.Cg-Rgs9^tm1.1(cre)Yql Tg(CAG-Ppard*E411P)#Als	MGI:5897185
cn2	Emx1^tm1(cre)Ito/Emx1^+ Rgs9^tm1.1(cre)Yql/Rgs9^+ Tg(HTT*97Q)IXwy/0	involves: 129P2/OlaHsd * FVB	MGI:5564936
cn3	Grin1^tm1Yql/Grin1^tm1Yql Rgs9^tm1.1(cre)Yql/Rgs9^+	involves: 129/Sv * BALB/c * C57BL/6	MGI:3693305
cn4	Rgs9^tm1.1(cre)Yql/Rgs9^+ Tg(HTT*97Q)IXwy/0	involves: FVB	MGI:5564939

Genotype
MGI:5897185

cn1

• Allelic Composition: Rgs9^{tm1.1(cre)Yql}/Rgs9⁺; Tg(CAG-Ppard*E411P)#Als/0
• Genetic Background: B6J.Cg-Rgs9^{tm1.1(cre)Yql} Tg(CAG-Ppard*E411P)#Als

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal motor capabilities/coordination/movement ( J:233176 )

• mice develop motor abnormalities

• in the cage-ledge test, mice cannot easily dismount from the cage ledge

limb grasping ( J:233176 )

• mice exhibit a prominent clasping phenotype

impaired coordination ( J:233176 )

• mice exhibit worse latency-to-fall times on the accelerating rotarod

decreased grip strength ( J:233176 )

• reduction in combined mesh and grip-strength

nervous system

abnormal medium spiny neuron morphology ( J:233176 )

• reduction in parvalbumin-immunoreactive neurons, indicating a decrease in numbers of striatal neurons

neurodegeneration ( J:233176 )

• degeneration of striatal neurons

skeleton

kyphosis ( J:233176 )

Genotype
MGI:5564936

cn2

• Allelic Composition: Emx1^tm1(cre)Ito/Emx1⁺; Rgs9^{tm1.1(cre)Yql}/Rgs9⁺; Tg(HTT*97Q)IXwy/0
• Genetic Background: involves: 129P2/OlaHsd * FVB

behavior/neurological

behavior/neurological phenotype ( J:208675 )

N • depression-like phenotype is not significantly different from wild-type but is significantly improved compared to BACHD (Tg(HTT*97Q)IXwy) mice

N • anxiety response is significantly improved compared to BACHD mice

N • significant, consistent improvement is observed in hypoactivity phenotype at 6 and 12 months compared to BACHD mice

N • significant, consistent improvement in coordination is observed at 6 and 12 months compared to BACHD mice

nervous system

nervous system phenotype ( J:208675 )

N • forebrain weight loss and cortical/striatal volume loss are improved relative to BACHD (Tg(HTT*97Q)IXwy) mice

Genotype
MGI:3693305

cn3

• Allelic Composition: Grin1^tm1Yql/Grin1^tm1Yql; Rgs9^{tm1.1(cre)Yql}/Rgs9⁺
• Genetic Background: involves: 129/Sv * BALB/c * C57BL/6

behavior/neurological

abnormal motor learning ( J:115299 )

• in rotarod tests, mutants do not show increased duration on rotating rod with successive trials, but control mice do; performance by wild-type on final 3 trials is significantly better than mutants which show impaired motor learning

nervous system

abnormal medium spiny neuron morphology ( J:115299 )

• medium spiny neurons have higher primary dendrite numbers (8.2) compared to wild-type (7)

abnormal excitatory postsynaptic currents ( J:115299 )

• NMDA-receptor mediated EPSCs are nearly abolished in mutants compared to wild-type

abnormal NMDA-mediated synaptic currents ( J:115299 )

• density of current activated by NMDA is reduced greatly in mutants vs controls

reduced long-term potentiation ( J:115299 )

• after high frequency stimulation, dorsomedial striatal slices from mutants do not display LTP

absent long-term depression ( J:115299 )

• brain slices that include the nucleus accumbens (NAc) from mutant mice do not show LTD with moderate-frequency stimulation of the NAc

Genotype
MGI:5564939

cn4

• Allelic Composition: Rgs9^{tm1.1(cre)Yql}/Rgs9⁺; Tg(HTT*97Q)IXwy/0
• Genetic Background: involves: FVB

behavior/neurological

behavioral despair ( J:208675 )

• significantly different from wild-type (similar to BACHD (Tg(HTT*97Q)IXwy) mice); little improvement is observed

increased anxiety-related response ( J:208675 )

• significantly different from wild-type (similar to BACHD mice); little improvement is observed

impaired coordination ( J:208675 )

• mice show significant rotarod impairment between 6 and 12 months relative to wild-type

decreased locomotor activity ( J:208675 )

• reduced movement is observed at 12 months relative to wild-type

nervous system

nervous system phenotype ( J:208675 )

N • evoked synaptic NMDA currents in medium spiny neurons (MSNs) in striatal slices from 13-15 month-old mice are not significantly different from wild-type; normalized amplitudes of currents are not significantly different

forebrain atrophy ( J:208675 )

• forebrain weight loss and striatal volume loss) are observed at 12 months compared to wild-type