Phenotypes associated with this allele

• Allele Symbol: Sall4^tm1Tre
• Allele Name: targeted mutation 1, Mathias Treier
• Allele ID: MGI:3692447
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Sall4^tm1Tre/Sall4^tm1Tre	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1	MGI:3693078
ht2	Sall4^tm1Tre/Sall4^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1	MGI:3693079
ht3	Sall4^tm1Tre/Sall4^tm2.1Tre	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1	MGI:3693084

Genotype
MGI:3693078

hm1

• Allelic Composition: Sall4^tm1Tre/Sall4^tm1Tre
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:115635 )

• no embryos are found in vivo at E5.5; empty implantation sites are regularly found

• no ICM-derived embryonic structures are recognizable in implantation sites where homozygous E3.5 embryos were transplanted into pseudopregnant females

embryo

abnormal embryonic tissue morphology ( J:115635 )

• few mitotic cells are detected in mutant explants after 5 days of culture

abnormal inner cell mass morphology ( J:115635 )

• ICM of embryos cultured in ES cell medium do not expand significantly compared to wild-type and heterozygous embryos

• after 5 days of culture, extraembryonic cell marker H19 is detected in homozygous explants, but not in controls; a significant portion of cells are positive for Cdx2, but this is not seen in control explants

• trophoblast giant cells are observed when ICM from E3.75 heterozygous blastocysts are cultured, but not in heterozygous ICMs

Genotype
MGI:3693079

ht2

• Allelic Composition: Sall4^tm1Tre/Sall4⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1

embryo

absent trophoblast giant cells ( J:115635 )

• no trophoblast giant cells are observed when ICM from E3.75 heterozygous blastocysts are cultured

• when cultured, an epithelial-like cell type is obtained, but not in cultured homozygous blastocysts

Genotype
MGI:3693084

ht3

• Allelic Composition: Sall4^tm1Tre/Sall4^tm2.1Tre
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:115635 )

• no prestreak embryos are found; empty implantations are regularly observed