Phenotypes associated with this allele

• Allele Symbol: Ednrb^tm1Yko
• Allele Name: targeted mutation 1, Yuri Kotelevtsev
• Allele ID: MGI:3693642
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ednrb^tm1Yko/Ednrb^tm1Yko Tg(Tek-cre)1Ywa/0	involves: 129P2/OlaHsd * Bkl:BKW * C57BL/6 * SJL	MGI:3804904
cn2	Ednrb^tm1Yko/Ednrb^tm1Yko Tg(AQP2-cre)2Dek/0	involves: C57BL/6 * CBA	MGI:3695302

Genotype
MGI:3804904

cn1

• Allelic Composition: Ednrb^tm1Yko/Ednrb^tm1Yko; Tg(Tek-cre)1Ywa/0
• Genetic Background: involves: 129P2/OlaHsd * Bkl:BKW * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

cardiovascular system phenotype ( J:135711 )

N • mutants do not develop cardiac hypertrophy on a regular diet or salt-sensitive hypertension

decreased vasodilation ( J:135711 )

• vasodilator response to S6c, a selective Ednrb agonist, is attenuated in mutant aortic rings compared to controls

• acetylcholine-induced vasodilation is impaired

• however, S6c-induced tracheal smooth muscle cell contraction is unaltered

homeostasis/metabolism

abnormal blood homeostasis ( J:135711 )

• plasma endothelin-1 concentration is increased

abnormal nitric oxide homeostasis ( J:135711 )

• decrease in endogenous release of NO

muscle

decreased vasodilation ( J:135711 )

• vasodilator response to S6c, a selective Ednrb agonist, is attenuated in mutant aortic rings compared to controls

• acetylcholine-induced vasodilation is impaired

• however, S6c-induced tracheal smooth muscle cell contraction is unaltered

Genotype
MGI:3695302

cn2

• Allelic Composition: Ednrb^tm1Yko/Ednrb^tm1Yko; Tg(AQP2-cre)2Dek/0
• Genetic Background: involves: C57BL/6 * CBA

cardiovascular system

increased systemic arterial blood pressure ( J:116002 )

• on a normal sodium diet, BP is 12.9 mmHg greater than control; on a high sodium diet, BP is additionally increased (+7 mmHg)

• BP is significantly greater than that observed in Ednra conditional mutants, and the additional increase seen with sodium loading is greater than Ednrb mutants but less than Edn1 conditional mutants

increased systemic arterial diastolic blood pressure ( J:116002 )

• on a normal sodium diet, BP is 8.9 mmHg greater than control; on a high sodium diet, BP is additionally increased (+5 mmHg)

increased systemic arterial systolic blood pressure ( J:116002 )

• mice have significantly elevated systolic blood pressure compared to controls as well as Ednra and Ednrb condition knockout mice on a normal sodium diet

• on a high sodium diet, systolic blood pressure increases significantly compared to controls; change in BP between normal and high sodium diets is much greater than in Ednra or Ednrb conditional mutants

homeostasis/metabolism

abnormal urine homeostasis ( J:116002 )

• urinary aldosterone excretion is lower than in controls

• on high sodium diet, urinary endothelin excretion is increased 3-fold over mutants on a normal sodium diet

abnormal urine sodium level ( J:116002 )

• after i.p. administration of 1.5 ml normal saline, urinary sodium excretion is substantially reduced in the first 2 hours after sodium loading; no difference is detected at 6 hours

decreased renin activity ( J:116002 )

• on normal sodium diet, plasma renin activity (PRA) is lower (~4-fold) than in controls

• on high sodium diet, PRA is lower (~3-fold) than in controls

renal/urinary system

abnormal urine homeostasis ( J:116002 )

• urinary aldosterone excretion is lower than in controls

• on high sodium diet, urinary endothelin excretion is increased 3-fold over mutants on a normal sodium diet

abnormal urine sodium level ( J:116002 )

• after i.p. administration of 1.5 ml normal saline, urinary sodium excretion is substantially reduced in the first 2 hours after sodium loading; no difference is detected at 6 hours

oliguria ( J:116002 )

• during initial 2 hours after sodium loading, urine output is reduced compared to controls (0.11 ml vs 0.75 ml)