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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Notch2tm1Frad
targeted mutation 1, Freddy Radtke
MGI:3695412
Summary 9 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Notch1tm1Agt/Notch1tm1Agt
Notch2tm1Frad/Notch2tm1Frad
Tg(Mx1-cre)1Cgn/0 		involves: 129 * BALB/c * C57BL/6 * CBA MGI:3758714
cn2
 		Notch1tm1Agt/Notch1+
Notch2tm1Frad/Notch2+
Tg(Tyr-cre)2Lru/0 		involves: 129 * BALB/c * C57BL/6 * DBA/2 MGI:3758742
cn3
 		Notch1tm1Agt/Notch1+
Notch2tm1Frad/Notch2tm1Frad
Tg(Tyr-cre)2Lru/0 		involves: 129 * BALB/c * C57BL/6 * DBA/2 MGI:3758743
cn4
 		Notch1tm1Agt/Notch1tm1Agt
Notch2tm1Frad/Notch2tm1Frad
Tg(Tyr-cre)2Lru/0 		involves: 129 * BALB/c * C57BL/6 * DBA/2 MGI:3758745
cn5
 		Notch1tm1Agt/Notch1tm1Agt
Notch2tm1Frad/Notch2tm1Frad
Ptf1atm1(cre)Hnak/Ptf1a+ 		involves: 129 * BALB/cJ MGI:3809249
cn6
 		Notch1tm1Agt/Notch1tm1Agt
Notch2tm1Frad/Notch2+
Tg(Tyr-cre)2Lru/0 		involves: 129/Sv * BALB/c * C57BL/6 * DBA/2 MGI:3758744
cn7
 		Notch2tm1Frad/Notch2tm1Frad
Tg(Tyr-cre)2Lru/0 		involves: 129/Sv * BALB/c * C57BL/6 * DBA/2 MGI:3758746
cn8
 		Notch2tm1Frad/Notch2+
Tg(Tyr-cre)2Lru/0 		involves: 129/Sv * BALB/c * C57BL/6 * DBA/2 MGI:3758747
cn9
 		Notch2tm1Frad/Notch2tm1Frad
Tg(Mx1-cre)1Cgn/0 		involves: BALB/c * C57BL/6 * CBA MGI:3758713


Genotype
MGI:3758714
cn1
Allelic
Composition		 Notch1tm1Agt/Notch1tm1Agt
Notch2tm1Frad/Notch2tm1Frad
Tg(Mx1-cre)1Cgn/0
Genetic
Background		 involves: 129 * BALB/c * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch1tm1Agt mutation (0 available); any Notch1 mutation (117 available)
Notch2tm1Frad mutation (0 available); any Notch2 mutation (99 available)
Tg(Mx1-cre)1Cgn mutation (7 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal T cell differentiation ( J:125373 )
• irradiated wild-type mice reconstituted with Notch1/2-null bone marrow progenitors analyzed at 8 weeks show block at earliest intrathymic precursor stage; immature B cells develop in the thymus recapitulating the Notch1-null phenotype
• cultured double-null hematopoietic stem cells, HSCs do not develop into T cell progenitors after 10 days
arrested T cell differentiation ( J:125373 )
• cultured double null HSCs display a block at the double negative 1 stage

immune system
abnormal T cell differentiation ( J:125373 )
• irradiated wild-type mice reconstituted with Notch1/2-null bone marrow progenitors analyzed at 8 weeks show block at earliest intrathymic precursor stage; immature B cells develop in the thymus recapitulating the Notch1-null phenotype
• cultured double-null hematopoietic stem cells, HSCs do not develop into T cell progenitors after 10 days
arrested T cell differentiation ( J:125373 )
• cultured double null HSCs display a block at the double negative 1 stage




Genotype
MGI:3758742
cn2
Allelic
Composition		 Notch1tm1Agt/Notch1+
Notch2tm1Frad/Notch2+
Tg(Tyr-cre)2Lru/0
Genetic
Background		 involves: 129 * BALB/c * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch1tm1Agt mutation (0 available); any Notch1 mutation (117 available)
Notch2tm1Frad mutation (0 available); any Notch2 mutation (99 available)
Tg(Tyr-cre)2Lru mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
pigmentation
diluted coat color ( J:116658 )
• at 8 weeks, dispersed gray hairs are observed

integument
diluted coat color ( J:116658 )
• at 8 weeks, dispersed gray hairs are observed




Genotype
MGI:3758743
cn3
Allelic
Composition		 Notch1tm1Agt/Notch1+
Notch2tm1Frad/Notch2tm1Frad
Tg(Tyr-cre)2Lru/0
Genetic
Background		 involves: 129 * BALB/c * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch1tm1Agt mutation (0 available); any Notch1 mutation (117 available)
Notch2tm1Frad mutation (0 available); any Notch2 mutation (99 available)
Tg(Tyr-cre)2Lru mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
pigmentation
pigmentation phenotype ( J:116658 )
N
• pigmentation mediated by non-follicular melanocytes such as in the ear or eye is not affected by this mutation
abnormal hair follicle melanocyte morphology ( J:116658 )
• when crossed to transgenic mice to visualize melanoblasts and melanocytes (dopachrome tautomerase - LacZ mice), at 9 days of age, a decreased number of melanocytes in the lower permanent portion of the hair follicle is observed; differences are greater at 3 and 4.5 weeks with most follicles lacking melanocytes
• mutant hair bulbs lack pigment after their first regeneration
diluted coat color ( J:116658 )
• coat is completetly gray with coat slightly lighter in color compared to mice missing both Notch2 alleles and one Notch1 allele
• dilution is progressive and increases with age; there is an increasing number of gray and white hairs with age such that at 7 days coat color is indistinguishable from controls, but at 7 months, coat is completely white

integument
abnormal hair follicle melanocyte morphology ( J:116658 )
• when crossed to transgenic mice to visualize melanoblasts and melanocytes (dopachrome tautomerase - LacZ mice), at 9 days of age, a decreased number of melanocytes in the lower permanent portion of the hair follicle is observed; differences are greater at 3 and 4.5 weeks with most follicles lacking melanocytes
• mutant hair bulbs lack pigment after their first regeneration
diluted coat color ( J:116658 )
• coat is completetly gray with coat slightly lighter in color compared to mice missing both Notch2 alleles and one Notch1 allele
• dilution is progressive and increases with age; there is an increasing number of gray and white hairs with age such that at 7 days coat color is indistinguishable from controls, but at 7 months, coat is completely white




Genotype
MGI:3758745
cn4
Allelic
Composition		 Notch1tm1Agt/Notch1tm1Agt
Notch2tm1Frad/Notch2tm1Frad
Tg(Tyr-cre)2Lru/0
Genetic
Background		 involves: 129 * BALB/c * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch1tm1Agt mutation (0 available); any Notch1 mutation (117 available)
Notch2tm1Frad mutation (0 available); any Notch2 mutation (99 available)
Tg(Tyr-cre)2Lru mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
pigmentation
diluted coat color ( J:116658 )
• hair pigmentation is even more reduced with complete absence of Notch1 and 2 in melanocytes: hair is almost white

integument
diluted coat color ( J:116658 )
• hair pigmentation is even more reduced with complete absence of Notch1 and 2 in melanocytes: hair is almost white




Genotype
MGI:3809249
cn5
Allelic
Composition		 Notch1tm1Agt/Notch1tm1Agt
Notch2tm1Frad/Notch2tm1Frad
Ptf1atm1(cre)Hnak/Ptf1a+
Genetic
Background		 involves: 129 * BALB/cJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch1tm1Agt mutation (0 available); any Notch1 mutation (117 available)
Notch2tm1Frad mutation (0 available); any Notch2 mutation (99 available)
Ptf1atm1(cre)Hnak mutation (1 available); any Ptf1a mutation (31 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
abnormal pancreatic acinus morphology ( J:139250 )
• at E14.5, the acinar compartment is reduced compared to in wild-type mice

endocrine/exocrine glands
abnormal pancreatic acinus morphology ( J:139250 )
• at E14.5, the acinar compartment is reduced compared to in wild-type mice
abnormal endocrine pancreas morphology ( J:139250 )
• the endocrine epithelium is mildly disrupted
• however, morphology of the islets is normal
decreased pancreatic islet number ( J:139250 )
• the number of islets is decreased compared to in wild-type mice
abnormal pancreas development ( J:139250 )
• mice exhibit a 25% decrease in the number of proliferating cells compared to in wild-type mice
abnormal branching involved in pancreas development ( J:139250 )
• at E13.5, pancreatic buds are less branched than in wild-type mice
abnormal pancreatic bud formation ( J:139250 )
• at E13.5, pancreatic buds are smaller than controls
small pancreas ( J:139250 )
• the pancreas is slightly smaller than in wild-type mice at P1




Genotype
MGI:3758744
cn6
Allelic
Composition		 Notch1tm1Agt/Notch1tm1Agt
Notch2tm1Frad/Notch2+
Tg(Tyr-cre)2Lru/0
Genetic
Background		 involves: 129/Sv * BALB/c * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch1tm1Agt mutation (0 available); any Notch1 mutation (117 available)
Notch2tm1Frad mutation (0 available); any Notch2 mutation (99 available)
Tg(Tyr-cre)2Lru mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
pigmentation
pigmentation phenotype ( J:116658 )
N
• pigmentation mediated by non-follicular melanocytes such as in the ear or eye is not affected by this mutation
diluted coat color ( J:116658 )
• coat is completetly gray

integument
diluted coat color ( J:116658 )
• coat is completetly gray




Genotype
MGI:3758746
cn7
Allelic
Composition		 Notch2tm1Frad/Notch2tm1Frad
Tg(Tyr-cre)2Lru/0
Genetic
Background		 involves: 129/Sv * BALB/c * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch2tm1Frad mutation (0 available); any Notch2 mutation (99 available)
Tg(Tyr-cre)2Lru mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
pigmentation
diluted coat color ( J:116658 )
• at 8 weeks, dispersed gray hairs are observed

integument
diluted coat color ( J:116658 )
• at 8 weeks, dispersed gray hairs are observed




Genotype
MGI:3758747
cn8
Allelic
Composition		 Notch2tm1Frad/Notch2+
Tg(Tyr-cre)2Lru/0
Genetic
Background		 involves: 129/Sv * BALB/c * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch2tm1Frad mutation (0 available); any Notch2 mutation (99 available)
Tg(Tyr-cre)2Lru mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
pigmentation
pigmentation phenotype ( J:116658 )
N
• loss of one allele has no affect on coat color; no graying or dilution is observed in 8-week old mice




Genotype
MGI:3758713
cn9
Allelic
Composition		 Notch2tm1Frad/Notch2tm1Frad
Tg(Mx1-cre)1Cgn/0
Genetic
Background		 involves: BALB/c * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch2tm1Frad mutation (0 available); any Notch2 mutation (99 available)
Tg(Mx1-cre)1Cgn mutation (7 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
hematopoietic system phenotype ( J:125373 )
N
• no ectopic B cell development in the thymus is observed after injection of mutant bone marrow progenitors into irradiated wild-type hosts
abnormal B cell differentiation ( J:125373 )
• hematopoietic stem cells do not develop in to B cells after 18 days in culture
absent marginal zone B cells ( J:125373 )
• loss of MZ B cells in spleen is detected

immune system
abnormal B cell differentiation ( J:125373 )
• hematopoietic stem cells do not develop in to B cells after 18 days in culture
absent marginal zone B cells ( J:125373 )
• loss of MZ B cells in spleen is detected




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory