Phenotypes associated with this allele

• Allele Symbol: Clec7a^tm1Gdb
• Allele Name: targeted mutation 1, Gordon D Brown
• Allele ID: MGI:3695710
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Clec7a^tm1Gdb/Clec7a^tm1Gdb	B6.129S6-Clec7a^tm1Gdb	MGI:5440672
hm2	Clec7a^tm1Gdb/Clec7a^tm1Gdb	involves: 129S6/SvEvTac * C57BL/6	MGI:3696859

Genotype
MGI:5440672

hm1

• Allelic Composition: Clec7a^tm1Gdb/Clec7a^tm1Gdb
• Genetic Background: B6.129S6-Clec7a^tm1Gdb

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

respiratory system

lung inflammation ( J:179011 , J:179011 )

• impaired inflammatory reactivity of alveolar macrophage (J:179011)

• impaired recruitment of macrophage to lungs (J:179011)

• highly susceptible to lung infection with Aspergillus fumigatus (J:179011)

• little antifungal activity in lung lavage fluid (J:179011)

immune system

abnormal circulating interleukin level ( J:179011 )

• IL-22 production in response to Aspergillus fumigatus challenge is severely reduced

decreased circulating interleukin-17 level ( J:179011 )

• lower levels of IL-17A after lung infection with Aspergillus fumigatus

lung inflammation ( J:179011 , J:179011 )

• impaired inflammatory reactivity of alveolar macrophage (J:179011)

• impaired recruitment of macrophage to lungs (J:179011)

• highly susceptible to lung infection with Aspergillus fumigatus (J:179011)

• little antifungal activity in lung lavage fluid (J:179011)

increased susceptibility to fungal infection ( J:179011 )

• highly susceptible to lung infection with Aspergillus fumigatus

homeostasis/metabolism

abnormal circulating interleukin level ( J:179011 )

• IL-22 production in response to Aspergillus fumigatus challenge is severely reduced

decreased circulating interleukin-17 level ( J:179011 )

• lower levels of IL-17A after lung infection with Aspergillus fumigatus

Genotype
MGI:3696859

hm2

• Allelic Composition: Clec7a^tm1Gdb/Clec7a^tm1Gdb
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:116669 )

• mice infected with sublethal dose of C. albicans have much lower survival rate compared to controls

immune system

abnormal neutrophil physiology ( J:116669 )

• cells do not have beta-glucan dependent recognition of unopsonized zymosan; respiratory burst is attenuated when cultured with unopsonized yeast, and response is not fully restored when opsonized zymosan particles are used

abnormal macrophage physiology ( J:116669 )

• thioglycollate-elicited macrophages show impaired recognition of zymosan (fungal beta-glucan particle); prior opsonization with mouse serum restores binding by macrophages

• elicited mutant macrophages show impairment of cytokine production when cultured with zymosan

• recognition of unopsonized yeast by macrophages is impaired compared to controls

abnormal cytokine secretion ( J:116669 )

• mutant macrophages show impaired production of Il10 and Il12 when cultured with zymosan

abnormal inflammatory response ( J:116669 )

• inflammatory response to zymosan fails to occur; defect is specific for fungal particles as other microbial stimuli elicit a response

• inflammatory response to yeast is not restored after opsonization of yeast with mouse serum, although fungal recognition is restored to macrophages

increased susceptibility to fungal infection ( J:116669 )

• mice infected with sublethal dose of C. albicans have much lower survival rate compared to controls

• at day 9 after infection, mutant mice have higher fungal burdens than controls in the gastrointestinal tract; enhanced systemic dissemination of C. albicans is evident within 24 hours of infection

• at time of death, mutant kidneys show enhanced fungal colonization by C. albicans, particularly in pelvic region with extension up renal tubules compared to controls which lack such colonization

• fungal hyphae extend through tubular epithelium into interstitium and are surrounded by acute neutrophilic inflammation (invasive candidiasis)

digestive/alimentary system

enlarged stomach ( J:116669 )

• mice which succumb to infection show macroscopic enlargement of stomach

abnormal digestive system physiology ( J:116669 )

• mice which succumb to infection have stomachs full of food, suggesting blockage of gastrointestinal tract

hematopoietic system

abnormal neutrophil physiology ( J:116669 )

• cells do not have beta-glucan dependent recognition of unopsonized zymosan; respiratory burst is attenuated when cultured with unopsonized yeast, and response is not fully restored when opsonized zymosan particles are used

abnormal macrophage physiology ( J:116669 )

• thioglycollate-elicited macrophages show impaired recognition of zymosan (fungal beta-glucan particle); prior opsonization with mouse serum restores binding by macrophages

• elicited mutant macrophages show impairment of cytokine production when cultured with zymosan

• recognition of unopsonized yeast by macrophages is impaired compared to controls