Phenotypes associated with this allele

• Allele Symbol: Clec7a^tm1Yiw
• Allele Name: targeted mutation 1, Yoichiro Iwakura
• Allele ID: MGI:3695711
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Clec7a^tm1Yiw/Clec7a^tm1Yiw	C.129P2-Clec7a^tm1Yiw	MGI:3696860
hm2	Clec7a^tm1Yiw/Clec7a^tm1Yiw	involves: 129P2/OlaHsd * C57BL/6	MGI:3716874
cx3	Clec7a^tm1Yiw/Clec7a^tm1Yiw Myd88^tm1Aki/Myd88^tm1Aki	involves: 129P2/OlaHsd * BALB/cA * C57BL/6J	MGI:3696862

Genotype
MGI:3696860

hm1

• Allelic Composition: Clec7a^tm1Yiw/Clec7a^tm1Yiw
• Genetic Background: C.129P2-Clec7a^tm1Yiw

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal macrophage physiology ( J:116670 )

• production of TNFa by thioglycollate-elicited macrophages treated with oxidized zymosan is abolished but is not altered with intact zymosan stimulation

• oxidized candidia does not elicit production of cytokines or ROS by mutant macrophages, but incubation with live C. albicans results in normal levels of production

• production of reactive oxygen species (ROS) by macrophages incubated with P. carinii is abolished compared to wild-type or Myd88 mutants

abnormal dendritic cell physiology ( J:116670 )

• production of Il12 and TNFa is abolished in bone marrow-derived dendritic cells (BMDC) after stimulation by Sparassis crispa glucan (SCG)

• SCG maturation of dendritic cells does not occur in mutants

abnormal cytokine secretion ( J:116670 )

• Il10 production is suppressed with LPS or zymosan stimulation of BMDCs compared to wild-type

increased susceptibility to fungal infection ( J:116670 )

• immunocompromised mutants infected with P. carinii have significantly more cysts in the lungs 24 hours after infection compared to wild-type

respiratory system

lung cyst ( J:116670 )

• immunocompromised mutants infected with P. carinii have significantly more cysts in the lungs 24 hours after infection compared to wild-type

hematopoietic system

abnormal macrophage physiology ( J:116670 )

• production of TNFa by thioglycollate-elicited macrophages treated with oxidized zymosan is abolished but is not altered with intact zymosan stimulation

• oxidized candidia does not elicit production of cytokines or ROS by mutant macrophages, but incubation with live C. albicans results in normal levels of production

• production of reactive oxygen species (ROS) by macrophages incubated with P. carinii is abolished compared to wild-type or Myd88 mutants

growth/size/body

lung cyst ( J:116670 )

• immunocompromised mutants infected with P. carinii have significantly more cysts in the lungs 24 hours after infection compared to wild-type

Genotype
MGI:3716874

hm2

• Allelic Composition: Clec7a^tm1Yiw/Clec7a^tm1Yiw
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

immune system phenotype ( J:122394 )

N • treatment of bone marrow derived dendritic cells with zymosan does not really affect production of Il-6, Il-12, and TNF

abnormal dendritic cell physiology ( J:122394 )

• after stimulation with NaClO-oxidized zymosan, cytokine responses are abolished in bone marrow-derived dendritic cells

Genotype
MGI:3696862

cx3

• Allelic Composition: Clec7a^tm1Yiw/Clec7a^tm1Yiw; Myd88^tm1Aki/Myd88^tm1Aki
• Genetic Background: involves: 129P2/OlaHsd * BALB/cA * C57BL/6J

immune system

abnormal dendritic cell physiology ( J:116670 )

• cytokine production elicited by zymosan or LPS stimulation is impaired

abnormal cytokine secretion ( J:116670 )

• LPS-induced cytokine production by BMDCs is almost abolished compared to controls and Myd88-sufficient mutants

• after stimulation with zymosan, TNF and Il12 production are significantly reduced in BMDCs of Myd88-deficient animals