Phenotypes associated with this allele

• Allele Symbol: Bcl3^tm1Rsch
• Allele Name: targeted mutation 1, Roland M Schmid
• Allele ID: MGI:3695737
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Bcl3^tm1Rsch/Bcl3^tm1Rsch	B6.Cg-Bcl3^tm1Rsch	MGI:3696889
hm2	Bcl3^tm1Rsch/Bcl3^tm1Rsch	involves: 129P2/OlaHsd * CD-1	MGI:3696888

Genotype
MGI:3696889

hm1

• Allelic Composition: Bcl3^tm1Rsch/Bcl3^tm1Rsch
• Genetic Background: B6.Cg-Bcl3^tm1Rsch

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

abnormal enzyme/coenzyme activity ( J:116724 )

• RNA polymerase II binding to the Il10 promoter and Il10transcription are significantly (~3-fold) higher than in control animals

immune system

abnormal macrophage physiology ( J:116724 )

• in mutants backcrossed 10 times, thioglycollate-elicited peritoneal macrophages stimulated with LPS show much higher levels of Il10 production than wild-type macrophages after 6 hours

• Il12 p70 synthesis is reduced compared to controls

abnormal cytokine secretion ( J:116724 )

• in mutants backcrossed 5 times, after LPS treatment, serum levels of Il12 p70 and IFNgamma are reduced, while TNFalpha levels are slightly elevated compared to controls: IFNgamma levels are ~17% of wild-type levels

• 1 hour after LPS treatment, Il10 levels are significantly higher in mutants

hematopoietic system

abnormal macrophage physiology ( J:116724 )

• in mutants backcrossed 10 times, thioglycollate-elicited peritoneal macrophages stimulated with LPS show much higher levels of Il10 production than wild-type macrophages after 6 hours

• Il12 p70 synthesis is reduced compared to controls

Genotype
MGI:3696888

hm2

• Allelic Composition: Bcl3^tm1Rsch/Bcl3^tm1Rsch
• Genetic Background: involves: 129P2/OlaHsd * CD-1

immune system

abnormal cytokine secretion ( J:116724 )

• 24 hours post-infection, serum levels of Il12 p70 and IFNgamma are reduced compared to controls, while TNFa levels are slightly elevated

abnormal innate immunity ( J:116724 )

increased susceptibility to bacterial infection ( J:116724 )

• mice infected i.p. with sublethal (to wild-type) dose of L. monocytogenes, mice dies within 8 days of inoculation while wild-type do not die; at higher bacterial doses, mutants show even greater susceptibility and die more rapidly