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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Gfi1tm1Wep
targeted mutation 1, William E Paul
MGI:3696049
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Gfi1tm1Wep/Gfi1tm1Wep B6.129-Gfi1tm1Wep MGI:3696769
cn2
 		Gfi1tm1Wep/Gfi1tm1Wep
Gfi1btm2Tmo/Gfi1btm2Tmo
Tg(Mx1-cre)1Cgn/? 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA MGI:4868585
cn3
 		Gfi1tm1Wep/Gfi1tm1Wep
Tg(Cd4-cre)1Cwi/0 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2 MGI:3696768


Genotype
MGI:3696769
hm1
Allelic
Composition		 Gfi1tm1Wep/Gfi1tm1Wep
Genetic
Background		 B6.129-Gfi1tm1Wep
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gfi1tm1Wep mutation (0 available); any Gfi1 mutation (31 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
growth/size/body
decreased body size ( J:117105 )

hematopoietic system
abnormal T cell proliferation ( J:117105 )
• cultured cells transfected with a retroviral cre show reduced expansion in Il2 by 3-fold over 2 days following antiCD3/CD28 priming
• after 2 days, in cre-transfected cultured cells, Il4-producing cells are decreased by ~13% compared to GFP transfected controls
abnormal definitive hematopoiesis ( J:117105 )
• have elevated population of Cd11b+ cells in spleen; this group was excluded from further studies and only 'healthy' animals were studied further

immune system
abnormal T cell proliferation ( J:117105 )
• cultured cells transfected with a retroviral cre show reduced expansion in Il2 by 3-fold over 2 days following antiCD3/CD28 priming
• after 2 days, in cre-transfected cultured cells, Il4-producing cells are decreased by ~13% compared to GFP transfected controls

cellular
abnormal T cell proliferation ( J:117105 )
• cultured cells transfected with a retroviral cre show reduced expansion in Il2 by 3-fold over 2 days following antiCD3/CD28 priming
• after 2 days, in cre-transfected cultured cells, Il4-producing cells are decreased by ~13% compared to GFP transfected controls




Genotype
MGI:4868585
cn2
Allelic
Composition		 Gfi1tm1Wep/Gfi1tm1Wep
Gfi1btm2Tmo/Gfi1btm2Tmo
Tg(Mx1-cre)1Cgn/?
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gfi1btm2Tmo mutation (0 available); any Gfi1b mutation (21 available)
Gfi1tm1Wep mutation (0 available); any Gfi1 mutation (31 available)
Tg(Mx1-cre)1Cgn mutation (7 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
decreased hematopoietic stem cell number ( J:167416 )
• numbers drastically reduced 15 days after the first pIpC injection to induce cre expression
• numbers restored by 40 days
• Gfi1b excision is incomplete




Genotype
MGI:3696768
cn3
Allelic
Composition		 Gfi1tm1Wep/Gfi1tm1Wep
Tg(Cd4-cre)1Cwi/0
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gfi1tm1Wep mutation (0 available); any Gfi1 mutation (31 available)
Tg(Cd4-cre)1Cwi mutation (10 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal T cell proliferation ( J:117105 )
• after 3-day priming iunder Th2 conditions and restimulation for 4 hours, an ~19% decrease in percentage of Il4-producing cells is seen compared to controls
abnormal T cell subpopulation ratio ( J:117105 )
• ratio of CD4 single positive to CD8 single positive thymocytes is slightly reduced
abnormal T-helper 2 physiology ( J:117105 )
• after infection with Schistosoma mansonii, 6-8 weeks later fewer lymph node cells make Il4 compared to controls
• after schistosoma egg antigen (SEA) stimulation for 5 days, ~3-fold fewer cells make Il4; production of other Th2 cytokines is reduced ~3-fold compared to control levels

immune system
abnormal T cell proliferation ( J:117105 )
• after 3-day priming iunder Th2 conditions and restimulation for 4 hours, an ~19% decrease in percentage of Il4-producing cells is seen compared to controls
abnormal T cell subpopulation ratio ( J:117105 )
• ratio of CD4 single positive to CD8 single positive thymocytes is slightly reduced
abnormal T-helper 2 physiology ( J:117105 )
• after infection with Schistosoma mansonii, 6-8 weeks later fewer lymph node cells make Il4 compared to controls
• after schistosoma egg antigen (SEA) stimulation for 5 days, ~3-fold fewer cells make Il4; production of other Th2 cytokines is reduced ~3-fold compared to control levels

cellular
abnormal T cell proliferation ( J:117105 )
• after 3-day priming iunder Th2 conditions and restimulation for 4 hours, an ~19% decrease in percentage of Il4-producing cells is seen compared to controls




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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory