Phenotypes associated with this allele

• Allele Symbol: Agrp^tm1Mws
• Allele Name: targeted mutation 1, Mark W Sleeman
• Allele ID: MGI:3696694
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Agrp^tm1Mws/Agrp^tm1Mws	B6.129S1-Agrp^tm1Mws	MGI:3697521
hm2	Agrp^tm1Mws/Agrp^tm1Mws	involves: 129S1/Sv * C57BL/6J	MGI:3697520

Genotype
MGI:3697521

hm1

• Allelic Composition: Agrp^tm1Mws/Agrp^tm1Mws
• Genetic Background: B6.129S1-Agrp^tm1Mws

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:117539 )

N • no change in absolute food intake

hyperactivity ( J:117539 )

• during the dark phase of the light cycle at 10 months of age

growth/size/body

decreased body weight ( J:117539 )

• Background Sensitivity: unlike mice on a mixed C57BL/6J and 129S1/Sv background which display normal body weight throughout life mice on an inbred C57BL/6J background display a decrease in body weight that becomes more pronounced with age

• significant decrease at 6 months of age but not at 3-5 months of age

• however, no difference is seen in body length

adipose tissue

decreased total body fat amount ( J:117539 )

• reduction in body weight is primarily the result of reduced fat mass

homeostasis/metabolism

abnormal blood homeostasis ( J:117539 )

increased circulating thyroxine level ( J:117539 )

• in males and females at 10 months of age

increased circulating triiodothyronine level ( J:117539 )

• in males and females at 10 months of age

decreased circulating leptin level ( J:117539 )

• 3-fold decrease at 10 months of age

increased core body temperature ( J:117539 )

• elevated core body temperature in 12 month old mice

abnormal energy expenditure ( J:117539 )

• at 10 months of age dark phase metabolic rate (VO2) is increased

decreased respiratory quotient ( J:117539 )

• reduced during the light phase of the light cycle at 10 months of age

decreased cholesterol level ( J:117539 )

• at 10 months of age

• however, levels of insulin, glucagon, glucose, triglycerides, nonesterfied fatty acids, and corticosterone are similar to wild-type

Genotype
MGI:3697520

hm2

• Allelic Composition: Agrp^tm1Mws/Agrp^tm1Mws
• Genetic Background: involves: 129S1/Sv * C57BL/6J

behavior/neurological

abnormal food intake ( J:117539 )

• no significant differences in 24 h food intake under normal conditions

• following a 24 h fast mice do not display increased food intake at 1 or 4 hours post fat but do show a slight increase in intake at 24 h post fast, unlike wild-type mice which display small but significant increases in food intake at 1, 4, and 24 h post fast

• intraperitoneal injection of 3 mg/kg melanotan II inhibits food intake for a longer time compared to wild-type mice

• repeated administration of 3 mg/kg melanotan II over 4 days prevents weight gain during this period in mutant but not wild-type mice

growth/size/body

growth/size/body region phenotype ( J:117539 )

N • Background Sensitivity: no significant difference in body weight unlike mice on an inbred C57BL/6J background