Phenotypes associated with this allele

• Allele Symbol: Tg(Fev-cre)1Esd
• Allele Name: transgene insertion 1, Evan S Deneris
• Allele ID: MGI:3696982
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Oprk1^tm2.1Kff/Oprk1^tm2.1Kff Tg(Fev-cre)1Esd/0	involves: 129 * C57BL/6 * SJL	MGI:5824893
cn2	Gata3^tm1Jfz/Gata3^tm1Jfz Tg(Fev-cre)1Esd/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:4837221
cn3	Fev^tm1Esd/Fev^tm2Esd Tg(Fev-cre)1Esd/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:4837211
cn4	Fev^tm1Esd/Fev^tm2Esd Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor^+ Tg(Fev-cre)1Esd/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:4837213
cn5	Crhr1^tm2Wrst/Crhr1^tm2Wrst Tg(Fev-cre)1Esd/0	involves: 129S2/SvPas * C57BL/6 * SJL	MGI:5294460
cn6	Lmx1b^tm1Zfc/Lmx1b^tm1Zfc Tg(Fev-cre)1Esd/0	involves: 129S7/SvEvBrd * C57BL/6 * SJL	MGI:3810753

Genotype
MGI:5824893

cn1

• Allelic Composition: Oprk1^tm2.1Kff/Oprk1^tm2.1Kff; Tg(Fev-cre)1Esd/0
• Genetic Background: involves: 129 * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal associative learning ( J:226396 )

• resistant to agonist U50,488-induced conditioned place aversion (CPA), however, like controls, evoked dopamine response is inhibited in response to agonist

Genotype
MGI:4837221

cn2

• Allelic Composition: Gata3^tm1Jfz/Gata3^tm1Jfz; Tg(Fev-cre)1Esd/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

nervous system

abnormal Raphe nucleus morphology ( J:165266 )

• decrease in the number of Tph immunoreactive cells in the dorsal Raphe nucleus

homeostasis/metabolism

decreased serotonin level ( J:165266 )

• in the dorsal Raphe nucleus

Genotype
MGI:4837211

cn3

• Allelic Composition: Fev^tm1Esd/Fev^tm2Esd; Tg(Fev-cre)1Esd/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

nervous system

nervous system phenotype ( J:165266 )

N • unlike germline null mice, the number of serotonergic neurons is normal at E11.5 and E12.5

Genotype
MGI:4837213

cn4

• Allelic Composition: Fev^tm1Esd/Fev^tm2Esd; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺; Tg(Fev-cre)1Esd/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

nervous system

abnormal somatosensory cortex morphology ( J:165266 )

• serotonergic innervation of the somatosensory cortex from dorsal raphe nucleus 5-HT neurons is significantly disrupted

abnormal innervation ( J:165266 )

• serotonergic innervation of the somatosensory cortex from dorsal raphe nucleus 5-HT neurons is significantly disrupted

abnormal serotonergic neuron morphology ( J:165266 )

• serotonergic innervation of the somatosensory cortex from dorsal raphe nucleus 5-HT neurons is significantly disrupted

abnormal nervous system electrophysiology ( J:165266 )

• neither high nor low concentrations of 8-Hydroxy-2-dipropylaminotetralin hydrobromide (8-OH-DPAT) elicit a change in baseline currents in labeled 5HT neurons

abnormal action potential ( J:165266 )

• YFP+ cells show increased spontaneous firing of action potentials

Genotype
MGI:5294460

cn5

• Allelic Composition: Crhr1^tm2Wrst/Crhr1^tm2Wrst; Tg(Fev-cre)1Esd/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * SJL

behavior/neurological

behavior/neurological phenotype ( J:176339 )

N • mice exhibit normal behavior in a forced swim test and auditory fear conditioning

increased anxiety-related response ( J:176339 )

• in a dark-light box, elevated plus maze, the novel object exploration, and modified hole board tests

• hypoactivity in the first 5 minutes of the open field test

decreased locomotor activity ( J:176339 )

• in the first 5 minutes of the open field test

nervous system

abnormal synaptic dopamine release ( J:176339 )

• mice exhibit decreased stress-induced dopamine release in the pre-frontal cortex compared with control mice

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:176339 )

N • mice exhibit normal basal and stress-induced corticosterone levels

Genotype
MGI:3810753

cn6

• Allelic Composition: Lmx1b^tm1Zfc/Lmx1b^tm1Zfc; Tg(Fev-cre)1Esd/0
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 * SJL

behavior/neurological

behavior/neurological phenotype ( J:116706 , J:170988 )

N • no apparent motor capability abnormalities (J:116706)

N • mice exhibit normal innate olfactory attractive or avoidance response, odor discrimination during habituation and dishabituation, and olfaction of general odors and pheromones (J:170988)

abnormal contextual conditioning behavior ( J:139720 )

• freezing behavior between electrical foot shocks significantly increases during the fourth or fifth interval

• when placed back into the environment after 30 minutes of rest, the mice freeze for almost as twice as long as controls

• the enhanced fear memory persisted even after 10 days when wild-type mice have largely ceased the behavior

• mice are not more prone to exhibit a freezing behavior when introduced to a novel environment and have normal nociceptive responses

• intracerebroventricular administration of serotonin restores normal freezing behaviors

abnormal long-term spatial reference memory ( J:139720 )

• after completing a 9-day training period in a morris water maze, the mice spend less time in the target quadrant, take a longer time to cross the platform location, and cross the target location less frequently compared to controls

• the deficiences are more pronounced 10 days after training compared to 1 day after training

decreased anxiety-related response ( J:139720 )

• mice spend at least 20% more time in the open arm of an elevated-plus maze test than wild-type mice indicating reduced anxiety

• mice also have about half the latency time between novelty presentation and feeding than exhibited by control mice

abnormal mating preference ( J:170988 )

♂ • male mice exhibit increased mounting of target male mice compared with control mice

♂ • male mice spend less time sniffing the female genital odor and more time sniffing the male genital odor compared with control mice

♂ • male mice spend less time above male bedding and less time above female bedding compared with control mice

♂ • male mice fail to exhibit an increase in mounting estrous female unlike wild-type mice

decreased vocalization ( J:170988 )

♂ • male mice exhibit increased ultrasonic vocalization towards males compared with control mice

♂ • however, male mice vocalization towards female are normal

nervous system

nervous system phenotype ( J:116706 )

N • expression levels of dopamine, dihydroxyphenyacetic acid, and norepinephrine in the brain and spinal cord are similar to control mice

abnormal Raphe nucleus morphology ( J:116706 , J:139720 )

• cytoarchitecture of the B1-B9 raphe nuclei is abnormal with the B6 nuclei (caudal nucleus raphe dorsalis) being absent (J:116706)

• serotonin-producing neurons are absent in the raphe magnus nucleus (J:139720)

• the distribution and density of tyrosine hydroxylase-positive neurons were unchanged (J:139720)

abnormal serotonergic neuron morphology ( J:116706 )

• at E12.5 serotonin staining is reduced and by E14.5 very few serotonin positive neurons are present in the brain

• in adult mice, only rare serotonin positive cells are found in raphe nuclei and no ascending or descending fibers are found in the rostral brain or spinal cord

• loss of serotonin cells appears to occur during postnatal stages

• however, no ectopic serotonin neurons are found and peripheral serotonin expression is similar to wild-type mice

abnormal long-term potentiation ( J:139720 )

• foot shock impairs subsequent hippocampal CA1 LTP responses in wild-type mice but not these mice as measured by fEPSP amplitude

• serotonin administration to hippocampal slices restores LTP responses

abnormal long-term depression ( J:139720 )

• foot shock impairs subsequent hippocampal CA1 LTD responses in wild-type mice but not these mice as measured by fEPSP amplitude

decreased neurotransmitter release ( J:139720 )

• adult mice lack essentially all central serotonin-producing neurons

• levels of serotonin are 10-fold lower than that of wild-type mice in the hippocampus, cortex, and striatum

• the levels of cells producing serotonin in the brain is 1% that of wild-type the levels of cells producing serotonin in the brain is 1% that of wild-type

taste/olfaction

taste/olfaction phenotype ( J:170988 )

N • mice exhibit normal innate olfactory attractive or avoidance response, odor discrimination during habituation and dishabituation, and olfaction of general odors and pheromones