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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Ppp4ctm1Tht
targeted mutation 1, Tse-Hua Tan
MGI:3697685
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Ppp4ctm1Tht/Ppp4ctm1Tht
Tg(Lck-cre)548Jxm/0
Tg(TcraTcrb)425Cbn/0 		involves: BALB/c * C57BL/6 * CBA MGI:3699364
cn2
 		Ppp4ctm1Tht/Ppp4ctm1Tht
Tg(Lck-cre)548Jxm/0 		involves: C57BL/6 * CBA MGI:3699363


Genotype
MGI:3699364
cn1
Allelic
Composition		 Ppp4ctm1Tht/Ppp4ctm1Tht
Tg(Lck-cre)548Jxm/0
Tg(TcraTcrb)425Cbn/0
Genetic
Background		 involves: BALB/c * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ppp4ctm1Tht mutation (0 available); any Ppp4c mutation (41 available)
Tg(Lck-cre)548Jxm mutation (2 available)
Tg(TcraTcrb)425Cbn mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal positive T cell selection ( J:117684 )
• only marginal increase in numbers of CD4 positive thymocytes is observed compared to TCR-transgenic mice
• CD4 SP/DP ratio is 0.29 compared to 0.46 in controls, indicating reduced positive selection
decreased double-positive T cell number ( J:117684 )
• a significant decrease in unfractionated, double-positive thymocyte numbers compared to single transgenic littermates abnormal CD4 T cell morphology
decreased single-positive T cell number ( J:117684 )
• significant decrease in SP cell numbers

immune system
abnormal positive T cell selection ( J:117684 )
• only marginal increase in numbers of CD4 positive thymocytes is observed compared to TCR-transgenic mice
• CD4 SP/DP ratio is 0.29 compared to 0.46 in controls, indicating reduced positive selection
decreased double-positive T cell number ( J:117684 )
• a significant decrease in unfractionated, double-positive thymocyte numbers compared to single transgenic littermates abnormal CD4 T cell morphology
decreased single-positive T cell number ( J:117684 )
• significant decrease in SP cell numbers




Genotype
MGI:3699363
cn2
Allelic
Composition		 Ppp4ctm1Tht/Ppp4ctm1Tht
Tg(Lck-cre)548Jxm/0
Genetic
Background		 involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ppp4ctm1Tht mutation (0 available); any Ppp4c mutation (41 available)
Tg(Lck-cre)548Jxm mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal T cell differentiation ( J:117684 )
• developmental block specifically occurs at the double negative (DN) stage
• decreased percentage of TCR betahiCD3hi mature thymocytes as well as decreased cell numbers are observed
abnormal positive T cell selection ( J:117684 )
• percentage of cells undergoing positive selection is decreased by ~60% compared to controls
• significant decrease in mature SP cell numbers
increased double-negative T cell number ( J:117684 )
• numbers of double negative (DN) cells are comparable to controls; however higher percentage of DN cells result
decreased double-positive T cell number ( J:117684 )
• a significant decrease in unfractionated double-positive thymocyte numbers compared to Tg-negative littermates; 70% decrease is observed accompanying the developmental block
decreased CD4-positive, alpha-beta T cell number ( J:117684 )
• significant decrease in numbers
decreased CD8-positive, alpha-beta T cell number ( J:117684 )
• significant decrease in numbers
decreased single-positive T cell number ( J:117684 )
• numbers of CD3+, Cd4+, and CD8+ T cells are decreased by ~50-60% in spleen and lymph nodes
abnormal T cell activation ( J:117684 )
• antigen-specific T cell activation is decreased, with decreased proliferation in response to antigen observed

hematopoietic system
abnormal T cell differentiation ( J:117684 )
• developmental block specifically occurs at the double negative (DN) stage
• decreased percentage of TCR betahiCD3hi mature thymocytes as well as decreased cell numbers are observed
abnormal positive T cell selection ( J:117684 )
• percentage of cells undergoing positive selection is decreased by ~60% compared to controls
• significant decrease in mature SP cell numbers
increased double-negative T cell number ( J:117684 )
• numbers of double negative (DN) cells are comparable to controls; however higher percentage of DN cells result
decreased double-positive T cell number ( J:117684 )
• a significant decrease in unfractionated double-positive thymocyte numbers compared to Tg-negative littermates; 70% decrease is observed accompanying the developmental block
decreased CD4-positive, alpha-beta T cell number ( J:117684 )
• significant decrease in numbers
decreased CD8-positive, alpha-beta T cell number ( J:117684 )
• significant decrease in numbers
decreased single-positive T cell number ( J:117684 )
• numbers of CD3+, Cd4+, and CD8+ T cells are decreased by ~50-60% in spleen and lymph nodes
abnormal T cell activation ( J:117684 )
• antigen-specific T cell activation is decreased, with decreased proliferation in response to antigen observed




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory