Phenotypes associated with this allele

• Allele Symbol: Tsix^tm3Jtl
• Allele Name: targeted mutation 3, Jeannie T Lee
• Allele ID: MGI:3698272
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tsix^tm3Jtl/Tsix^tm3Jtl	involves: 129	MGI:3699217
ht2	Tsix^tm3Jtl/Tsix^+	involves: 129	MGI:3699214
ot3	Tsix^tm3Jtl/Y	involves: 129	MGI:3699216

Genotype
MGI:3699217

hm1

• Allelic Composition: Tsix^tm3Jtl/Tsix^tm3Jtl
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

prenatal lethality, complete penetrance ( J:117335 )

♀ • no female homozygotes are born

Genotype
MGI:3699214

ht2

• Allelic Composition: Tsix^tm3Jtl/Tsix⁺
• Genetic Background: involves: 129

mortality/aging

decreased survivor rate ( J:117335 )

♀ • only 10-20% of mutants survive to adulthood, when the allele is transmitted maternally

embryonic lethality at implantation, incomplete penetrance ( J:117335 )

♀ • mutants die during the implantation stage when allele is transmitted maternally; ~20% of mutant blastocysts bearing maternal allele fail to attach in culture and ~60% show sparse trophoblast outgrowth and degenerate after several days in culture, whereas wild-type blastocysts show robust growth for up to 7 days

♀ • ex vivo, no obvious defects are observed during preimplantation stage

♀ • paternal transmission has no effect on viability

reproductive system

decreased litter size ( J:117335 )

♀ • female mutants produce smaller litters

cellular

abnormal imprinting ( J:117335 )

♀ • live births carrying mutant allele are decreased (males: 40 wild-type, 11 mutant and females: 66 wild-type, 6 mutant whereas ratio of mutants to wild-type for both sexes expected is 50:50); 75-90% of mutants bearing maternal allele die

Genotype
MGI:3699216

ot3

• Allelic Composition: Tsix^tm3Jtl/Y
• Genetic Background: involves: 129

mortality/aging

decreased survivor rate ( J:117335 )

♂ • only 10-20% of mutants survive to adulthood, when the allele is transmitted maternally

embryonic lethality at implantation, incomplete penetrance ( J:117335 )

♂ • with maternal transmission of mutant allele, viability is dramatically decreased

♂ • ~20% of mutant blastocysts bearing maternal allele fail to attach in culture and ~60% show sparse trophoblast outgrowth and degenerate after several days in culture, whereas wild-type blastocysts show robust growth for up to 7 days

♂ • paternal transmission has no effect on viability

cellular

abnormal imprinting ( J:117335 )

♂ • live births carrying mutant allele are decreased (males: 40 wild-type, 11 mutant and females: 66 wild-type, 6 mutant whereas ratio of mutants to wild-type for both sexes expected is 50:50); 75-90% of mutants bearing maternal allele die