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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Rr100tm2.1Welm
targeted mutation 2.1, Wilfried Ellmeier
MGI:3698404
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Rr100tm2.1Welm/Rr100tm2.1Welm involves: 129P2/OlaHsd MGI:4454315
hm2
 		Rr100tm2.1Welm/Rr100tm2.1Welm involves: 129P2/OlaHsd * C57BL/6 MGI:3698423
ht3
 		Rr100tm2.1Welm/Rr100+ involves: 129P2/OlaHsd * AKR.1 * C57BL/6 MGI:3698425
cx4
 		Rr100tm2.1Welm/Rr100tm2.1Welm
Tcratm1Mom/Tcratm1Mom 		involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6 MGI:3698424
cx5
 		Rr100tm2.1Welm/Rr100tm2.1Welm
Patz1tm1.1Welm/Patz1tm1.1Welm 		involves: 129P2/OlaHsd * C57BL/6 MGI:4454318


Genotype
MGI:4454315
hm1
Allelic
Composition		 Rr100tm2.1Welm/Rr100tm2.1Welm
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal T cell differentiation ( J:158947 )
• a subpopulation of CD4 SP thymocytes derived in vivo, from transferred E13.5 day livers show surface expression similar to double positive (DP) thymocytes (except lacking CD8 expression)

immune system
immune system phenotype ( J:158947 )
N
• no defect is detected in competitive repopulation assays, suggesting the decrease in T cell numbers is a result of decreased body size
abnormal T cell differentiation ( J:158947 )
• a subpopulation of CD4 SP thymocytes derived in vivo, from transferred E13.5 day livers show surface expression similar to double positive (DP) thymocytes (except lacking CD8 expression)




Genotype
MGI:3698423
hm2
Allelic
Composition		 Rr100tm2.1Welm/Rr100tm2.1Welm
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal T cell differentiation ( J:113136 )
• there is dramatic change in CD4 vs CD8 expression profile compared to wild-type
• a subpopulation of CD4 SP thymocytes show surface expression similar to double positive (DP) thymocytes (except lacking CD8 expression)
• intraepithelial lymphocytes (IEL) lose CD8a expression on TCR gamma/delta lineage cells whereas Cc8atm1.1Litt cells have normal CD8a levels
decreased double-positive T cell number ( J:113136 )
• percentage is decreased compared to controls (from 84% to 65%)
abnormal T cell subpopulation ratio ( J:113136 )
• in thymic lobes after 3 days in culture, ratio of CD4 single positive cells to double positive cells is much higher than in cultured heterozygous organs; percentage of immature CD8 SP thymocytes is seen
• "CD8-negative" SP cells emerge to a significant extent after 7 days of culture, coincident with development of double positive cells
increased CD4-positive, alpha-beta T cell number ( J:113136 )
• increased CD4 single positive (SP) cell number from 11% in controls to 29%
decreased CD8-positive, alpha-beta T cell number ( J:113136 )
• percentage (1.8%) and absolute numbers of CD8+ SP cells (1.9 x106) in thymus of mutants are decreased compared to controls (4.5%, 2.8 x x106 respectively)
• CD8+ T cell numbers in periphery (lymph nodes and spleen) are decreased vs controls

immune system
abnormal T cell differentiation ( J:113136 )
• there is dramatic change in CD4 vs CD8 expression profile compared to wild-type
• a subpopulation of CD4 SP thymocytes show surface expression similar to double positive (DP) thymocytes (except lacking CD8 expression)
• intraepithelial lymphocytes (IEL) lose CD8a expression on TCR gamma/delta lineage cells whereas Cc8atm1.1Litt cells have normal CD8a levels
decreased double-positive T cell number ( J:113136 )
• percentage is decreased compared to controls (from 84% to 65%)
abnormal T cell subpopulation ratio ( J:113136 )
• in thymic lobes after 3 days in culture, ratio of CD4 single positive cells to double positive cells is much higher than in cultured heterozygous organs; percentage of immature CD8 SP thymocytes is seen
• "CD8-negative" SP cells emerge to a significant extent after 7 days of culture, coincident with development of double positive cells
increased CD4-positive, alpha-beta T cell number ( J:113136 )
• increased CD4 single positive (SP) cell number from 11% in controls to 29%
decreased CD8-positive, alpha-beta T cell number ( J:113136 )
• percentage (1.8%) and absolute numbers of CD8+ SP cells (1.9 x106) in thymus of mutants are decreased compared to controls (4.5%, 2.8 x x106 respectively)
• CD8+ T cell numbers in periphery (lymph nodes and spleen) are decreased vs controls




Genotype
MGI:3698425
ht3
Allelic
Composition		 Rr100tm2.1Welm/Rr100+
Genetic
Background		 involves: 129P2/OlaHsd * AKR.1 * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal T cell differentiation ( J:113136 )
• mice with one mutant Cd8a allele and a wild-type AKR.1 derived allele have ""CD8-negative"" single positive (SP) thymocytes expressing no CD8 alpha or beta

immune system
abnormal T cell differentiation ( J:113136 )
• mice with one mutant Cd8a allele and a wild-type AKR.1 derived allele have ""CD8-negative"" single positive (SP) thymocytes expressing no CD8 alpha or beta




Genotype
MGI:3698424
cx4
Allelic
Composition		 Rr100tm2.1Welm/Rr100tm2.1Welm
Tcratm1Mom/Tcratm1Mom
Genetic
Background		 involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rr100tm2.1Welm mutation (0 available); any Rr100 mutation (0 available)
Tcratm1Mom mutation (6 available); any Tcra mutation (101 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal T cell morphology ( J:113136 )
• mice have population of abnormal "CD8-negative" SP thymocytes

immune system
abnormal T cell morphology ( J:113136 )
• mice have population of abnormal "CD8-negative" SP thymocytes




Genotype
MGI:4454318
cx5
Allelic
Composition		 Rr100tm2.1Welm/Rr100tm2.1Welm
Patz1tm1.1Welm/Patz1tm1.1Welm
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Patz1tm1.1Welm mutation (0 available); any Patz1 mutation (55 available)
Rr100tm2.1Welm mutation (0 available); any Rr100 mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:158947 )
N
• unlike mice with a wild-type copy of Patz, null mice have no defects in T cell differentiation




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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory