All Phenotypes Hsp90b1<tm1.1Zhli> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Hsp90b1^tm1Zhli/Hsp90b1^tm1.1Zhli Lyz2^tm1(cre)Ifo/Lyz2⁺	involves: 129P2/OlaHsd * 129S/SvEv	MGI:3700816
cn2	Hsp90b1^tm1Zhli/Hsp90b1^tm1.1Zhli Tg(Ddx4-cre)1Dcas/0	involves: 129S/SvEv * FVB	MGI:6885740

Allelic Composition	Hsp90b1^tm1Zhli/Hsp90b1^tm1.1Zhli Lyz2^tm1(cre)Ifo/Lyz2⁺
Genetic Background	involves: 129P2/OlaHsd * 129S/SvEv

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hsp90b1^tm1.1Zhli mutation (0 available); any Hsp90b1 mutation (35 available)
Hsp90b1^tm1Zhli mutation (0 available); any Hsp90b1 mutation (35 available)
Lyz2^tm1(cre)Ifo mutation (14 available); any Lyz2 mutation (40 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:118427 )

• mutants die from vascular collapse, intravascular coagulation and organ ischemia after gram-negative bacterial infection

• wild-type mice are more susceptible to LPS-induced sepsis than mutants, showing 63% mortality from endotoxemia compared to 17% mortality of mutants after 4 days

immune system

abnormal macrophage physiology ( J:118427 )

• macrophages isolated from mutants do not respond to ligands for TLR5 and TLR7

• less Il-6 or Il-12p40 in response to LPS or CpG olignucleotide stimulation, respectively, are secreted by mutant macrophages than by wild-type

• TNFalpha release is reduced in response to poly I:C stimulation compared to wild-type

abnormal cytokine secretion ( J:118427 )

• 7-week old mice produce less cytokines like Il-6, Il-12p40 and TNFalpha than wild-type mice after LPS treatment

abnormal inflammatory response ( J:118427 )

• mutant show reduced response to LPS compared to wild-type

increased susceptibility to bacterial infection ( J:118427 )

• 3 days following infection by Listeria monocytogenes, mutants show significant weight loss and reduction of serum IL-12p40 and also show higher bacterial burden than wild-type

• 60-90% of spleen white pulp follicles are destroyed after 3 days with severe loss of lymphocytes

• numerous necrotic foci and abscesses are observed in infected mutant livers

hematopoietic system

abnormal macrophage physiology ( J:118427 )

• macrophages isolated from mutants do not respond to ligands for TLR5 and TLR7

• less Il-6 or Il-12p40 in response to LPS or CpG olignucleotide stimulation, respectively, are secreted by mutant macrophages than by wild-type

• TNFalpha release is reduced in response to poly I:C stimulation compared to wild-type

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

reproductive system phenotype ( J:168319 )

N	• adult males show normal testis weight-to-body weight ratio relative to control males

abnormal sperm midpiece morphology ( J:168319 )

• fluorescent HSPA5 (an ER chaperone) and TO-PRO-3 (DNA) staining revealed strong HSPA5 localization within the abnormal sperm head rather than at the level of the intermediate piece as observed in wild-type sperm

• streptavidin-FITC (biotinylated proteins in mitochondria) and TO-PRO-3 (DNA) staining revealed a stronger streptavidin-FITC labeling concentrated at the abnormal sperm head, unlike in wild-type sperm

abnormal sperm head morphology ( J:168319 )

• all cauda epididymal sperm show head distortions including abnormal heads with (i) a rather elongated head, ii) a rounded-globular head, and iii) an enlarged-deformed head

• 86% of abnormal sperm heads include types (ii) and (iii)

• normal hook-shaped sperm heads are never observed

abnormal acrosome morphology ( J:168319 )

• fluorescent Lens culinaris agglutinin (LCA)-fluorescein isothiocyanate (FITC) (glycoconjugates) and TO-PRO-3 (DNA) staining revealed a diffuse and variable labeling of the acrosome, unlike in wild-type sperm

globozoospermia ( J:168319 )

• ~40% of sperm heads are rounded-globular

enlarged sperm head ( J:168319 )

• ~40% of sperm heads are enlarged and deformed

asthenozoospermia ( J:168319 )

• cauda epididymal sperm are almost immobile

male infertility ( J:168319 )

• adult males are infertile

• however, mating behavior is normal

cellular