Phenotypes associated with this allele

• Allele Symbol: H2-M3^tm1Crw
• Allele Name: targeted mutation 1, Chung-Ru Wang
• Allele ID: MGI:3701255
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	H2-M3^tm1Crw/H2-M3^tm1Crw	B6.129-H2-M3^tm1Crw	MGI:3701873

Genotype
MGI:3701873

hm1

• Allelic Composition: H2-M3^tm1Crw/H2-M3^tm1Crw
• Genetic Background: B6.129-H2-M3^tm1Crw

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

decreased activated T cell number ( J:118960 )

• on day 5 after L. monocytogenes infection, mutants show a slightly reduced number of activated (CD8+CD44+) T cells in contrast to wild-type, but on day 7, when peak numbers are found in wild-type, percentage of activated T cells is decreased ~50% in mutants

abnormal CD8-positive, alpha beta T cell morphology ( J:118960 )

• after infection with Listeria monocytogenes, no tetramer+ CD8+ T cells are observed after 7 days in null mice, while substantial cells are detected in wild-type

• in vitro, when splenocytes and hepatic leukocytes from mutants 7 days after infection stimulated with heat-killed bacteria, frequency and total numbers of Listeria-specific CD8+ T cells producing interferon gamma are reduced vs wild-type

impaired natural killer cell mediated cytotoxicity ( J:118960 )

• splenocytes and hepatic leukocytes from infected mutants are able to lyse YAC-1 cells (NK targets) in vitro with reduced percentages compared to controls

abnormal cytotoxic T cell physiology ( J:118960 )

• in infected mutants, no H2-M3/LemA-specific response is detected after injection of B6 splenocytes pulsed with this peptide, but cytolytic activity to ovalbumin-coated target B6 cells is comparable to wild-type

decreased macrophage nitric oxide production ( J:118960 )

• 3 days after LM infection, splenocytes and hepatic leukocytes from mutants produce less nitric oxide in vitro than cells from littermates

immune system

decreased activated T cell number ( J:118960 )

• on day 5 after L. monocytogenes infection, mutants show a slightly reduced number of activated (CD8+CD44+) T cells in contrast to wild-type, but on day 7, when peak numbers are found in wild-type, percentage of activated T cells is decreased ~50% in mutants

abnormal CD8-positive, alpha beta T cell morphology ( J:118960 )

• after infection with Listeria monocytogenes, no tetramer+ CD8+ T cells are observed after 7 days in null mice, while substantial cells are detected in wild-type

• in vitro, when splenocytes and hepatic leukocytes from mutants 7 days after infection stimulated with heat-killed bacteria, frequency and total numbers of Listeria-specific CD8+ T cells producing interferon gamma are reduced vs wild-type

abnormal cytotoxic T cell physiology ( J:118960 )

• in infected mutants, no H2-M3/LemA-specific response is detected after injection of B6 splenocytes pulsed with this peptide, but cytolytic activity to ovalbumin-coated target B6 cells is comparable to wild-type

decreased interferon-gamma secretion ( J:118960 )

• reduced interferon gamma is detected in cultures of splenocytes from infected mice

abnormal innate immunity ( J:118960 )

• response is reduced in mutants compared to wild-type mice

impaired natural killer cell mediated cytotoxicity ( J:118960 )

• splenocytes and hepatic leukocytes from infected mutants are able to lyse YAC-1 cells (NK targets) in vitro with reduced percentages compared to controls

decreased macrophage nitric oxide production ( J:118960 )

• 3 days after LM infection, splenocytes and hepatic leukocytes from mutants produce less nitric oxide in vitro than cells from littermates

increased susceptibility to bacterial infection ( J:118960 )

• infection of mice with a sublethal dose of L. monocytogenes (LM), mutants show substantial higher bacterial burdens in the spleen and liver at early time points (3 and 5 days) compared to wild-type

• mice are more susceptible to infection at higher bacterial doses compared to wild-type

homeostasis/metabolism

decreased macrophage nitric oxide production ( J:118960 )

• 3 days after LM infection, splenocytes and hepatic leukocytes from mutants produce less nitric oxide in vitro than cells from littermates