cellular
• mutant MEFs show a much higher frequency of chromosomal aberrations compared to wild-type cells, with increased incidence of fragmented chromosomes, broken or fragmented sister chromosomes compared to none in wild-type cells
• other abnormalities are increased incidence of spontaneous random chromosomal translocations, and a ring chromosome possibly resulting from a breakage and fusion event
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aneuploidy
(
J:118989
)
• 73% of mutant metaphase MEFs differ from normal diploid chromosome number of 40, while wild-type MEFs have 32% frequency of aneuploidy
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• mutant MEFs have a 4.5-fold higher frequency of spontaneous sister chromatid exchange compared to wild-type
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• MEFs treated with ionizing radiation show less proliferation than wild-type MEFs
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• MEFs from mutants show increased frequency of spontaneous double-strand DNA breaks (DSB); ~50% of MEFs show gamma H2AX foci, indicating an elevated load of unrepaired DSBs
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