Phenotypes associated with this allele

• Allele Symbol: Gnl3^{Gt(W223E05)Wrst}
• Allele Name: gene trap W223E05, Wolfgang Wurst
• Allele ID: MGI:3701318
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gnl3^Gt(W223E05)Wrst/Gnl3^Gt(W223E05)Wrst	Not Specified	MGI:3702078
ht2	Gnl3^Gt(W223E05)Wrst/Gnl3^+	Not Specified	MGI:3702080
cx3	Gnl3^Gt(W223E05)Wrst/Gnl3^Gt(W223E05)Wrst Trp53^tm1Tyj/Trp53^tm5Tyj	involves: 129S2/SvPas * 129S4/SvJae	MGI:3702081

Genotype
MGI:3702078

hm1

• Allelic Composition: Gnl3^{Gt(W223E05)Wrst}/Gnl3^{Gt(W223E05)Wrst}
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality before implantation, incomplete penetrance ( J:118144 )

• most embryos die before implantation

• at E4.5 all homozygotes show varying degrees of deterioration

embryonic lethality between implantation and somite formation, complete penetrance ( J:118144 )

• a few highly abnormal and underdeveloped embryos survive until just after implantation

embryo

failure of blastocyst to hatch from the zona pellucida ( J:118144 )

• no hatched embryos are found at E4.5

embryonic growth arrest ( J:118144 )

• at the blastocyst stage

abnormal blastocyst morphology ( J:118144 )

• at E3 embryos contain about half as many nuclei as control littermates

• from E3 to E3.5 no increase in the number of cells in the embryo is seen compared to a doubling of the number of cells in control littermates over the same time span

• however nuclei are morphologically normal suggesting no defect in mitosis is present and no increase in the number of apoptotic cell is detected

cellular

abnormal cell cycle ( J:118144 )

• at E3.0 - E3.5 cells in the blastocyst fail to enter the S-phase of the cell cycle

decreased cell proliferation ( J:118144 )

• at E3.0 - E3.5 almost no BrdU incorporation is seen indicating a significant impairment in proliferation in the whole embryo

• from E3 to E3.5 no increase in the number of cells in the embryo is seen compared to a doubling of the number of cells in control littermates over the same time span

Genotype
MGI:3702080

ht2

• Allelic Composition: Gnl3^{Gt(W223E05)Wrst}/Gnl3⁺
• Genetic Background: Not Specified

cellular

decreased cell proliferation ( J:118144 )

• late, but not early, passages of MEFs show a slight but reproducible decrease in cell proliferation

Genotype
MGI:3702081

cx3

• Allelic Composition: Gnl3^{Gt(W223E05)Wrst}/Gnl3^{Gt(W223E05)Wrst}; Trp53^tm1Tyj/Trp53^tm5Tyj
• Genetic Background: involves: 129S2/SvPas * 129S4/SvJae

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:118144 )

• loss of Trp53 expression does not alter or delay the lethality

embryo

embryonic growth arrest ( J:118144 )

• loss of Trp53 expression does not alter or delay the overall phenotype

cellular

decreased cell proliferation ( J:118144 )

• loss of Trp53 expression does not alter or delay the impairment of cell proliferation