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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Ightm1Cys
targeted mutation 1, Jason G Cyster
MGI:3701508
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
 		Ightm1Cys/Igh+
Tg(Igk)5Cys/0 		B6.Cg-Ightm1Cys Tg(Igk)5Cys MGI:3702732
cx2
 		Ightm1Cys/Igh+
Tg(Igk)5Cys/0
Tg(UBC-GFP)30Scha/Tg(UBC-GFP)30Scha 		involves: 129X1/SvJ * C57BL/6 * CBA MGI:3702731


Genotype
MGI:3702732
cx1
Allelic
Composition		 Ightm1Cys/Igh+
Tg(Igk)5Cys/0
Genetic
Background		 B6.Cg-Ightm1Cys Tg(Igk)5Cys
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ightm1Cys mutation (0 available); any Igh mutation (44 available)
Tg(Igk)5Cys mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:118931 )
N
• mutant B cells undergo class-switching, maturation and somatic hypermutation
abnormal B cell physiology ( J:118931 )
• double mutants show a significantly higher percentage (15.6%) of peripheral blood cells that bind hen egg lysozyme (HEL) compared to wild-type (0.016%) or Ightm1Cys knock-in mice (1.06%)
• about 70% of total B cells are HEL-binding

hematopoietic system
abnormal B cell physiology ( J:118931 )
• double mutants show a significantly higher percentage (15.6%) of peripheral blood cells that bind hen egg lysozyme (HEL) compared to wild-type (0.016%) or Ightm1Cys knock-in mice (1.06%)
• about 70% of total B cells are HEL-binding




Genotype
MGI:3702731
cx2
Allelic
Composition		 Ightm1Cys/Igh+
Tg(Igk)5Cys/0
Tg(UBC-GFP)30Scha/Tg(UBC-GFP)30Scha
Genetic
Background		 involves: 129X1/SvJ * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ightm1Cys mutation (0 available); any Igh mutation (44 available)
Tg(Igk)5Cys mutation (0 available)
Tg(UBC-GFP)30Scha mutation (9 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:118931 )
N
• when germinal center B cells from these mice are transferred to wild-type recipients that are then immunized with duck egg lysozyme conjugated with ovalbumin, the Ightm1Cys, Tg(Igk)5Cys GC B cells undergo apparently normal class switching, maturation and somatic hypermutation
• class switching to IgG2a and IgG2b is observed by day 7 after immunization
• an increased number of somatic hypermutations are detected as the germinal center response progressed as is selection for particular mutations by day 14




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory