Analysis Tools
growth/size/body
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• at weaning, mice of both genders have a total body mass that is ~15-20% lower than that of wild-type or heterozygous controls
• a body mass difference is evident from birth and persists until 6 months of age
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muscle
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• at 15 weeks of age, the proportion of soleus muscle fibers staining with an antibody against slow myosin HC increased to 40% relative to 31% in wild-type controls
• at 3 months of age, SDS-PAGE revealed an increase in the ratio of slow to fast myosin HC isoforms as shown by an increase in the proportion of type I (slow) and disappearance of type IIb isoforms
• a shift from fast to slow fibers in soleus muscle is already evident at 3 weeks of age
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• at 15 weeks of age, males show a significant reduction in soleus weight relative to wild-type controls
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• at 15 weeks of age, males show a significant reduction in TA weight relative to wild-type controls
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• at 8-10 months of age, all muscle types examined (vastus and rectus femoris, gastrocnemius, and extensor digitorum longus) show increased variability in the size of myofibers relative to wild-type controls
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• at 8-10 months of age, all muscle types examined (vastus and rectus femoris, gastrocnemius, and extensor digitorum longus) show an increased proportion of myofibers with centrally located nuclei
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• a shift from fast to slow fiber types is evident in soleus muscle even in young mice
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• mice older than 6 months of age develop a mild myopathy
• at 8-10 months of age, all muscle types examined display increased variability in the size of myofibers as well as an increased proportion of myofibers harboring centrally located nuclei
• however, no change is observed in the localization of laminin or dystrophin in muscle tissue
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limbs/digits/tail
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• at 15 weeks of age, the proportion of soleus muscle fibers staining with an antibody against slow myosin HC increased to 40% relative to 31% in wild-type controls
• at 3 months of age, SDS-PAGE revealed an increase in the ratio of slow to fast myosin HC isoforms as shown by an increase in the proportion of type I (slow) and disappearance of type IIb isoforms
• a shift from fast to slow fibers in soleus muscle is already evident at 3 weeks of age
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• at 15 weeks of age, males show a significant reduction in soleus weight relative to wild-type controls
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• at 15 weeks of age, males show a significant reduction in TA weight relative to wild-type controls
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