Phenotypes associated with this allele

• Allele Symbol: Tcrb^tm3Slek
• Allele Name: targeted mutation 3, Barry P Sleckman
• Allele ID: MGI:3702579
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tcrb^tm3Slek/Tcrb^tm3Slek	Not Specified	MGI:3703051
ht2	Tcrb^tm2(Tcrb)Fwa/Tcrb^tm3Slek	involves: 129	MGI:3776877

Genotype
MGI:3703051

hm1

• Allelic Composition: Tcrb^tm3Slek/Tcrb^tm3Slek
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

arrested T cell differentiation ( J:119552 )

• development is blocked at the double negative (DN) stage

decreased T cell number ( J:119552 )

• mice have reduced numbers of peripheral T cells relative to wild-type

immune system

arrested T cell differentiation ( J:119552 )

• development is blocked at the double negative (DN) stage

decreased T cell number ( J:119552 )

• mice have reduced numbers of peripheral T cells relative to wild-type

Genotype
MGI:3776877

ht2

• Allelic Composition: Tcrb^tm2(Tcrb)Fwa/Tcrb^tm3Slek
• Genetic Background: involves: 129

immune system

abnormal T cell receptor beta chain V(D)J recombination ( J:132446 )

• 57% of single positive thymocytes express a Vbeta14 Tcr-beta chain compared to only 7.5% in age-matched control mice

• there is a compensatory 50% decrease in the number of single positive thymocytes that express Vbeta5 and Vbeta8 chains

• 65% percent of alpha-beta T cells in lymph nodes express a Vbeta14 chain compared to 7% in control mice

• expression of Vbeta8 and Vbeta5 chains on alpha-beta T cells in lymph nodes is reduced by 2.5- and 2- fold respectively

• higher expression of Vbeta14 is due to a higher level of primary Vbeta14 rearrangement to Dbeta1Jbeta1 complexes as revealed by hybridoma analysis

hematopoietic system

abnormal T cell receptor beta chain V(D)J recombination ( J:132446 )

• 57% of single positive thymocytes express a Vbeta14 Tcr-beta chain compared to only 7.5% in age-matched control mice

• there is a compensatory 50% decrease in the number of single positive thymocytes that express Vbeta5 and Vbeta8 chains

• 65% percent of alpha-beta T cells in lymph nodes express a Vbeta14 chain compared to 7% in control mice

• expression of Vbeta8 and Vbeta5 chains on alpha-beta T cells in lymph nodes is reduced by 2.5- and 2- fold respectively

• higher expression of Vbeta14 is due to a higher level of primary Vbeta14 rearrangement to Dbeta1Jbeta1 complexes as revealed by hybridoma analysis