Phenotypes associated with this allele

• Allele Symbol: Pdx1^tm3Cvw
• Allele Name: targeted mutation 3, Christopher VE Wright
• Allele ID: MGI:3702893
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pdx1^tm3Cvw/Pdx1^tm3Cvw	involves: 129S6/SvEvTac * C57BL/6 * DBA * FVB/N	MGI:3703989
ht2	Pdx1^tm3Cvw/Pdx1^+	involves: 129S6/SvEvTac * C57BL/6 * DBA * FVB/N	MGI:3703990
ht3	Pdx1^tm2Cvw/Pdx1^tm3Cvw	involves: 129/Sv * C57BL/6 * DBA * FVB/N	MGI:3703988

Genotype
MGI:3703989

hm1

• Allelic Composition: Pdx1^tm3Cvw/Pdx1^tm3Cvw
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * DBA * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:104617 )

• mice survive at least 7 days, but none reach weaning age

endocrine/exocrine glands

abnormal pancreas development ( J:104617 )

• dorsal pancreas is hypoplastic

• glucagon- and insulin-producing cells are decreased in number

• dorsal pancreatic remnant dorsal to duodenum forms as cystic structure lined with simple cuboidal epithelium

digestive/alimentary system

abnormal enteroendocrine cell morphology ( J:104617 )

• GIP-producing cell numbers are reduced compared to wild-type controls

• pancreatic endocrine cell differentiation is defective; all four major endocrine cell types are detected, but only as small clusters or single cells rather than tightly apposed ductal structures

Genotype
MGI:3703990

ht2

• Allelic Composition: Pdx1^tm3Cvw/Pdx1⁺
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * DBA * FVB/N

endocrine/exocrine glands

increased PP cell number ( J:104617 )

• islets contain higher numbers of pancreatic polypeptide (PP)-producing cells compared to wild-type mice

increased pancreatic alpha cell number ( J:104617 )

• islets contain higher numbers of glucagon-producing cells compared to wild-type mice

disorganized pancreatic islets ( J:104617 )

• glucagon-producing cells are often intermixed with beta cells, rather than being peripherally located as in wild-type

homeostasis/metabolism

increased circulating glucose level ( J:104617 )

• at 10 weeks of age, mice have higher glucose levels than wild-type

decreased circulating insulin level ( J:104617 )

• levels are significantly decreased at 10 weeks

• at 8-11 weeks, plasma insulin is low 15 minutes after glucose challenge, with an overall smaller and delayed increase compared to controls

impaired glucose tolerance ( J:104617 )

• at 8-11 weeks of age, mice have severely impaired glucose clearance compared to conrol littermates; levels are >600 mg/dl glucose in many animals

Genotype
MGI:3703988

ht3

• Allelic Composition: Pdx1^tm2Cvw/Pdx1^tm3Cvw
• Genetic Background: involves: 129/Sv * C57BL/6 * DBA * FVB/N

mortality/aging

postnatal lethality, complete penetrance ( J:104617 )

• most mice die by P9, and all are dead by P17

growth/size/body

postnatal growth retardation ( J:104617 )

• by 2 days postnatal, mice show growth retardation

endocrine/exocrine glands

exocrine pancreatic insufficiency ( J:104617 )

• dorsal outgrowth found in place of pancreas lacks insulin, somatostatin, and PP, as well as exocrine markers like amylase

abnormal pancreas development ( J:104617 )

• pancreas is replaced by small rudiment protruding from duodenal wall; rudiment has glucagon-producing cells at tips of ductular tree that shows limited branching

• at E12, dorsal pancreatic bud is smaller with no evidence of branching morphogenesis compared to wild-type; no separate ventral pancreatic bud is present

digestive/alimentary system

abnormal enteroendocrine cell morphology ( J:104617 )

• GIP-producing cells are decreased 63.4% compared to Pdx^tm4.1Cvw homozygotes

exocrine pancreatic insufficiency ( J:104617 )

• dorsal outgrowth found in place of pancreas lacks insulin, somatostatin, and PP, as well as exocrine markers like amylase

homeostasis/metabolism

dehydration ( J:104617 )

• by 2 days postnatal, mice show dehydration