Phenotypes associated with this allele

• Allele Symbol: Lemd3^{Gt(XST167)Byg}
• Allele Name: gene trap XST167, BayGenomics
• Allele ID: MGI:3703328
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lemd3^Gt(XST167)Byg/Lemd3^Gt(XST167)Byg	involves: 129P2/OlaHsd * C57BL/6	MGI:3703873

Genotype
MGI:3703873

hm1

• Allelic Composition: Lemd3^{Gt(XST167)Byg}/Lemd3^{Gt(XST167)Byg}
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:119566 )

• number of homozygous embryos at E11.5 is lower than expected and none are recovered at E12.5 or later

• some embryos die in the middle of turning (prior to E10)

cardiovascular system

abnormal vascular development ( J:119566 )

• at E10, embryos lack fine capillary network in the cranial, branchial, cardiac and intersomitic regions

• extracellular matrix (ECM) deposition is aberrant around endothelial cells, impairing migration

abnormal dorsal aorta morphology ( J:119566 )

• at E10, smooth muscle cells are lacking around the dorsal aorta; endothelial cells in mutant dorsal aortae are not supported by mural cells, resulting in collapse of the dorsal aorta

• tight junctions between endothelial cells form normally

abnormal vitelline vasculature morphology ( J:119566 )

• yolk sac vasculature is formed in mutant yolk sacs, but is highly dilated

• at E10, yolk sac shows primary capillary plexus, but no branching from pre-existing vessels

enlarged pericardium ( J:119566 )

• at E10, some homozygotes show an enlarged pericardium and clumps of red blood cells

cellular

abnormal cell nucleus morphology ( J:119566 )

• most mutant mesenchymal nuclei are irregularly shaped compared to wild-type nuclei which are tense and smooth; some mesenchymal cells display herniated nuclei and a small frequency (~1%) have expanded perinuclear space

increased embryonic tissue cell apoptosis ( J:119566 )

• massive apoptosis is detected in homozygous embryos, particularly in mesenchymal tissues, compared to wild-type littermates

embryo

abnormal vitelline vasculature morphology ( J:119566 )

• yolk sac vasculature is formed in mutant yolk sacs, but is highly dilated

• at E10, yolk sac shows primary capillary plexus, but no branching from pre-existing vessels

increased embryonic tissue cell apoptosis ( J:119566 )

• massive apoptosis is detected in homozygous embryos, particularly in mesenchymal tissues, compared to wild-type littermates

pale yolk sac ( J:119566 )

• at E10, homozygous embryos have smaller, paler yolk sacs