Phenotypes associated with this allele

• Allele Symbol: Tg(H2-K-AKR1B1)1Tj
• Allele Name: transgene insertion 1, University of Tokushima School of Medicine
• Allele ID: MGI:3703741
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Ldlr^tm1Her/Ldlr^tm1Her Tg(H2-K-AKR1B1)1Tj/0	involves: 129S7/SvEvBrd * C57BL/6 * DBA/2	MGI:3798391
cx2	Ldlr^tm1Her/Ldlr^+ Tg(H2-K-AKR1B1)1Tj/0	involves: 129S7/SvEvBrd * C57BL/6 * DBA/2	MGI:3798393
tg3	Tg(H2-K-AKR1B1)1Tj/0	involves: C57BL/6 * DBA/2	MGI:3703752

Genotype
MGI:3798391

cx1

• Allelic Composition: Ldlr^tm1Her/Ldlr^tm1Her; Tg(H2-K-AKR1B1)1Tj/0
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

hyperglycemia ( J:100916 )

• streptozotocin (STZ) treatment leads to 3-fold higher level of blood glucose in mice fed a high fat diet

• these high glucose levels persist for at least 12 weeks after STZ treatment

abnormal circulating cholesterol level ( J:100916 )

• streptozotocin (STZ) induced diabetes in mice fed a high fat diet leads to blood cholesterol levels that are twice those of non-diabetic mice 6 weeks post-induction

• blood cholesterol levels increase to three times greater than in non-diabetic mice 8 weeks after STZ treatment

cardiovascular system

increased susceptibility to atherosclerosis ( J:100916 )

• non-diabetic mice fed a high fat diet have a total lesion area of 10.7% in their aortas compared to 8.0% in Ldlr ^tm1Her homozyogotes that do not carry the transgene

• diabetic mice fed a high fat diet for 6 weeks have a total aortic lesion area of 37.5% compared to 23.4% lesion area in diabetic Ldlr ^tm1Her homozyogotes that do not carry the transgene

• these differences in aortic lesion area between transgenic and non-transgenic Ldlr ^tm1Her homozyogotes increase the longer the mice are on a high fat diet

• the greatest differences are observed in the aortic arch of diabetic mice where there is a 65% increase in the lesion area 6 weeks after disease induction compared to Ldlr ^tm1Her homozyogotes that do not carry the transgene

immune system

abnormal macrophage physiology ( J:100916 )

• peritoneal macrophages have a higher uptake of acetylated LDL compared macrophages from Ldlr ^tm1Her homozyogotes that do not carry the transgene

• mean uptake of acetylated-LDL is 5.27 micrograms/mg cellular protein vs 1.18 micrograms/mg cellular protein in controls

hematopoietic system

abnormal macrophage physiology ( J:100916 )

• peritoneal macrophages have a higher uptake of acetylated LDL compared macrophages from Ldlr ^tm1Her homozyogotes that do not carry the transgene

• mean uptake of acetylated-LDL is 5.27 micrograms/mg cellular protein vs 1.18 micrograms/mg cellular protein in controls

Genotype
MGI:3798393

cx2

• Allelic Composition: Ldlr^tm1Her/Ldlr⁺; Tg(H2-K-AKR1B1)1Tj/0
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 * DBA/2

homeostasis/metabolism

hyperglycemia ( J:100916 )

• streptozotocin (STZ) treatment leads to 3-fold higher level of blood glucose in mice fed a high fat diet

• these high glucose levels persist for at least 12 weeks after STZ treatment

cardiovascular system

increased susceptibility to atherosclerosis ( J:100916 )

• in mice made diabetic by STZ treatment and fed a cholesterol/cholic acid containing diet for 12 weeks, total aortic lesion area is doubled compared to Ldlr ^tm1Her heterozygotes that do not carry the transgene

• the presence of the transgene causes no difference in lesion size in non-diabetic mice

Genotype
MGI:3703752

tg3

• Allelic Composition: Tg(H2-K-AKR1B1)1Tj/0
• Genetic Background: involves: C57BL/6 * DBA/2

vision/eye

cataract ( J:120049 )

• 7/15 transgenic mice developed cataracts when fed a 20% galactose diet for 7 days; 2/7 only displayed cataracts while 5 mice also developed occlusion of the retinochoroidal vessels

renal/urinary system

abnormal renal glomerular capsule morphology ( J:120049 )

• multiple fibrinous deposits were observed in the Bowman's capsule of many glomeruli in 4/5 mice fed a normal diet

• mice fed a 20% galactose diet show fibrinous deposits in Bowman's capsules of kidneys while littermates fed the same diet show no histopathological changes

cardiovascular system

abnormal blood vessel morphology ( J:120049 )

• 5/15 mice fed a 20% galactose diet for 7 days developed cataracts and occlusion of retinochoroidal vessels while another 2 mice developed vascular occlusion only

homeostasis/metabolism

abnormal glucose homeostasis ( J:120049 )

• sorbitol concentration in liver, kidney, and muscle after intake of 30% glucose chow for 5 days increases by 1.7, 2.1, and 2.0, respectively compared to control littermates; levels with normal diet are not significantly different between transgenic mice and controls

abnormal enzyme/coenzyme activity ( J:120049 )

• activity of purified AR from transgenic mouse muscle is 5-6 fold higher than in muscle from littermates