Phenotypes associated with this allele

• Allele Symbol: Tg(Pbsn-Tag)12T7fRjm
• Allele Name: transgene insertion 12T7f, Robert J Matusik
• Allele ID: MGI:3706555
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(Pbsn-Hpn,-GFP)DVv/0 Tg(Pbsn-Tag)12T7fRjm/0	involves: C57BL/6J * CBA * CD-1	MGI:4358595
tg2	Tg(Pbsn-Tag)12T7fRjm/0	involves: CD-1	MGI:4358596

Genotype
MGI:4358595

cx1

• Allelic Composition: Tg(Pbsn-Hpn,-GFP)DVv/0; Tg(Pbsn-Tag)12T7fRjm/0
• Genetic Background: involves: C57BL/6J * CBA * CD-1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

increased prostate gland adenocarcinoma incidence ( J:92562 )

♂ • at 21 weeks, mice show adenocarcinoma with extensive glandular differentiation and focal areas of cribiform lesions; often neoplastic glands invade the periprostatic stroma

♂ • half of affected male animals, tumor cells show nuclear and chromatin patterns as well ase 'rosette formation' suggestive of neuroendocrine (NE) differentiation

increased prostate intraepithelial neoplasia incidence ( J:92562 )

♂

increased metastatic potential ( J:92562 )

• by 21 weeks of age, 55% of mice develop prominent metastasis with increased levels of transgenic Hpn detected in liver, lung, and bone

• metastases have tightly packed cells displaying nuclear mold and high nuclear/cytoplasmic ratios

reproductive system

abnormal prostate gland morphology ( J:92562 )

♂ • prostate glands display areas with disruption and disorganization of epithelial structures

♂ • cell proliferation is similar to single transgenic Tg(Pbsn-Tag)12T7fRjm mice, but apoptosis is increased by 4-fold

enlarged prostate gland ( J:92562 )

♂ • prostate glands are enlarged, showing similar size and weight to single transgenic mice at 21 weeks

increased prostate gland adenocarcinoma incidence ( J:92562 )

♂ • at 21 weeks, mice show adenocarcinoma with extensive glandular differentiation and focal areas of cribiform lesions; often neoplastic glands invade the periprostatic stroma

♂ • half of affected male animals, tumor cells show nuclear and chromatin patterns as well ase 'rosette formation' suggestive of neuroendocrine (NE) differentiation

increased prostate intraepithelial neoplasia incidence ( J:92562 )

♂

endocrine/exocrine glands

abnormal prostate gland morphology ( J:92562 )

♂ • prostate glands display areas with disruption and disorganization of epithelial structures

♂ • cell proliferation is similar to single transgenic Tg(Pbsn-Tag)12T7fRjm mice, but apoptosis is increased by 4-fold

enlarged prostate gland ( J:92562 )

♂ • prostate glands are enlarged, showing similar size and weight to single transgenic mice at 21 weeks

increased prostate gland adenocarcinoma incidence ( J:92562 )

♂ • at 21 weeks, mice show adenocarcinoma with extensive glandular differentiation and focal areas of cribiform lesions; often neoplastic glands invade the periprostatic stroma

♂ • half of affected male animals, tumor cells show nuclear and chromatin patterns as well ase 'rosette formation' suggestive of neuroendocrine (NE) differentiation

increased prostate intraepithelial neoplasia incidence ( J:92562 )

♂

Genotype
MGI:4358596

tg2

• Allelic Composition: Tg(Pbsn-Tag)12T7fRjm/0
• Genetic Background: involves: CD-1

neoplasm

increased prostate gland tumor incidence ( J:48623 )

♂ • 100% of males develop prostate tumors

♂ • after the development of epithelial hyperplasia, reactive stromal proliferation develops and then the tumor progresses to high-grade dysplasia with regions of carcinoma in situ, and on occasion, contain cribriform PIN-like lesions and regions of adenocarcinoma

increased prostate gland adenocarcinoma incidence ( J:48623 )

♂ • areas of microglandular patterns mimicking invasive prostate cancer, indicating localized intraprostatic invasion and areas of distinct lymph-vascular space invasion is seen indicating locally invasive carcinoma

♂ • males castrated at 7 weeks of age do not develop prostatic tumors or show prostate regrowth

♂ • tumors regress in males castrated at 11 week of age and androgen treatment restores tumor growth, indicating that tumor growth is androgen-dependent

increased prostate intraepithelial neoplasia incidence ( J:48623 , J:92562 )

♂ • cribriform PIN-like lesions (J:48623)

♂ • at 21 weeks, typical prostatic intraepithelial neoplastic lesions (PIN lesions) (J:92562)

reproductive system

abnormal prostate gland morphology ( J:92562 )

♂ • stromal cell hyperplasia is observed at 21 weeks

enlarged prostate gland ( J:92562 )

♂ • males exhibit massive enlargement of the prostate gland by 21 weeks

prostate gland hyperplasia ( J:48623 )

♂ • clusters of hyperplastic epithelial cells in the prostate are first seen around 5 weeks of age

♂ • between 8 and 10 weeks of age, the epithelium of the dorsolateral prostate is completely replaced by hyperplasia and surrounded by a deep layer of proliferating stroma; the stromal cells eventually fill in the interstitial space

♂ • the dorsolateral and anterior lobes show the most dramatic changes and the ventral lobe remains small but shows evidence of epithelial cell hyperplasia

increased prostate gland tumor incidence ( J:48623 )

♂ • 100% of males develop prostate tumors

♂ • after the development of epithelial hyperplasia, reactive stromal proliferation develops and then the tumor progresses to high-grade dysplasia with regions of carcinoma in situ, and on occasion, contain cribriform PIN-like lesions and regions of adenocarcinoma

increased prostate gland adenocarcinoma incidence ( J:48623 )

♂ • areas of microglandular patterns mimicking invasive prostate cancer, indicating localized intraprostatic invasion and areas of distinct lymph-vascular space invasion is seen indicating locally invasive carcinoma

♂ • males castrated at 7 weeks of age do not develop prostatic tumors or show prostate regrowth

♂ • tumors regress in males castrated at 11 week of age and androgen treatment restores tumor growth, indicating that tumor growth is androgen-dependent

increased prostate intraepithelial neoplasia incidence ( J:48623 , J:92562 )

♂ • cribriform PIN-like lesions (J:48623)

♂ • at 21 weeks, typical prostatic intraepithelial neoplastic lesions (PIN lesions) (J:92562)

endocrine/exocrine glands

abnormal prostate gland morphology ( J:92562 )

♂ • stromal cell hyperplasia is observed at 21 weeks

enlarged prostate gland ( J:92562 )

♂ • males exhibit massive enlargement of the prostate gland by 21 weeks

prostate gland hyperplasia ( J:48623 )

♂ • clusters of hyperplastic epithelial cells in the prostate are first seen around 5 weeks of age

♂ • between 8 and 10 weeks of age, the epithelium of the dorsolateral prostate is completely replaced by hyperplasia and surrounded by a deep layer of proliferating stroma; the stromal cells eventually fill in the interstitial space

♂ • the dorsolateral and anterior lobes show the most dramatic changes and the ventral lobe remains small but shows evidence of epithelial cell hyperplasia

increased prostate gland tumor incidence ( J:48623 )

♂ • 100% of males develop prostate tumors

♂ • after the development of epithelial hyperplasia, reactive stromal proliferation develops and then the tumor progresses to high-grade dysplasia with regions of carcinoma in situ, and on occasion, contain cribriform PIN-like lesions and regions of adenocarcinoma

increased prostate gland adenocarcinoma incidence ( J:48623 )

♂ • areas of microglandular patterns mimicking invasive prostate cancer, indicating localized intraprostatic invasion and areas of distinct lymph-vascular space invasion is seen indicating locally invasive carcinoma

♂ • males castrated at 7 weeks of age do not develop prostatic tumors or show prostate regrowth

♂ • tumors regress in males castrated at 11 week of age and androgen treatment restores tumor growth, indicating that tumor growth is androgen-dependent

increased prostate intraepithelial neoplasia incidence ( J:48623 , J:92562 )

♂ • cribriform PIN-like lesions (J:48623)

♂ • at 21 weeks, typical prostatic intraepithelial neoplastic lesions (PIN lesions) (J:92562)

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
prostate cancer		DOID:10283	OMIM:176807 OMIM:300147 OMIM:300704 OMIM:601518 OMIM:602759 OMIM:608656 OMIM:608658 OMIM:609299 OMIM:609558 OMIM:610321 OMIM:610997 OMIM:611100 OMIM:611868 OMIM:611928 OMIM:611955 OMIM:611958 OMIM:611959	J:48623