All Phenotypes Rad51c<tm1.1Sks> MGI Mouse

abnormal reproductive system morphology ( J:119835 )

abnormal female meiosis ( J:119835 )

• 60-75% of infertile females oocytes have poorly structured metaphase plates compared to 30% in controls (Rad51c^tm1Sks heterozygotes)

• however, chromosomes align normally along the metaphase I plate and there are no apparent defects in pairing and cohesion of chromosomes

• oocytes of hormone-treated infertile females have a variety of chromosome abnormalities at metaphase II including precocious separation of chromatids

• in oocytes with precocious separation of chromatids some chromatids are accentric and a few have two centromeres

• 20% of oocytes from superovulated infertile females have greater than 20 chromosomes and some have 20 or fewer pairs of centromeres

abnormal female reproductive system morphology ( J:119835 )

absent corpus luteum ( J:119835 )

• in infertile females at 12 weeks of age

• however, corpora lutea are present after superovulation of infertile females

abnormal spermatogenesis ( J:119835 )

• at 12 weeks of age, in infertile mice most tubules contain no mature sperm, few spermatids and multiple layers of spermatocytes

• even though testes morphology and cell composition improves with age males are still infertile up to 8 months of age

oligozoospermia ( J:119835 )

• infertile mice have few mature spermatozoa in tubules

abnormal spermatocyte morphology ( J:119835 )

• infertile mice have increased number of apoptotic spermatocytes in the testes

• spermatocyte distribution is shifted towards early stages of prophase I (leptotene and early to mid zygotene) with fewer cells in the late zygotene or pachytene stages

arrest of male meiosis ( J:119835 )

• infertile mice have impaired spermatogenesis during meiosis I and rarely progress beyond metaphase I

• during meiosis, cells display unpaired autosomal univalents and persistence of double strand breaks

• however, no signs of precocious separation of sister chromatids is detected

abnormal male reproductive system morphology ( J:119835 )

abnormal seminiferous tubule morphology ( J:119835 )

• at 4 weeks of age, tubules are deformed and often almost devoid of germ cells

• at 12 weeks of age, in infertile mice most tubules contain no mature sperm, few spermatids and multiple layers of spermatocytes

• at 12 weeks of age, interstitial tissue appears hypertrophied in both fertile and infertile mice

decreased testis weight ( J:119835 )

• testes of infertile males are smaller and weighed less at 8-10 weeks of age than fertile males

abnormal reproductive system physiology ( J:119835 )

decreased litter size ( J:119835 )

• at E8.5 fewer embryos are removed from the uterine horns of superovulated infertile females compared to superovulated controls and 7 of 10 of these embryos are developmentally abnormal

infertility ( J:119835 )

• some males and females are infertile

• however, sexual activity is normal and fertile mice produce normal sized litters compared to controls

female infertility ( J:119835 )

• 11.6% of females are infertile

• however, infertile females injected with pregnant mare serum and human chorionic gonadotropin can be successfully mated

male infertility ( J:119835 )

• 36.6% of males are infertile

• even though testes morphology and cell composition improves with age males are still infertile up to 8 months of age

absent corpus luteum ( J:119835 )

• in infertile females at 12 weeks of age

• however, corpora lutea are present after superovulation of infertile females

abnormal seminiferous tubule morphology ( J:119835 )

• at 4 weeks of age, tubules are deformed and often almost devoid of germ cells

• at 12 weeks of age, in infertile mice most tubules contain no mature sperm, few spermatids and multiple layers of spermatocytes

• at 12 weeks of age, interstitial tissue appears hypertrophied in both fertile and infertile mice

decreased testis weight ( J:119835 )

• testes of infertile males are smaller and weighed less at 8-10 weeks of age than fertile males

oligozoospermia ( J:119835 )

• infertile mice have few mature spermatozoa in tubules

abnormal spermatocyte morphology ( J:119835 )

• infertile mice have increased number of apoptotic spermatocytes in the testes

• spermatocyte distribution is shifted towards early stages of prophase I (leptotene and early to mid zygotene) with fewer cells in the late zygotene or pachytene stages

abnormal female meiosis ( J:119835 )

• 60-75% of infertile females oocytes have poorly structured metaphase plates compared to 30% in controls (Rad51c^tm1Sks heterozygotes)

• however, chromosomes align normally along the metaphase I plate and there are no apparent defects in pairing and cohesion of chromosomes

• oocytes of hormone-treated infertile females have a variety of chromosome abnormalities at metaphase II including precocious separation of chromatids

• in oocytes with precocious separation of chromatids some chromatids are accentric and a few have two centromeres

• 20% of oocytes from superovulated infertile females have greater than 20 chromosomes and some have 20 or fewer pairs of centromeres

arrest of male meiosis ( J:119835 )

• infertile mice have impaired spermatogenesis during meiosis I and rarely progress beyond metaphase I

• during meiosis, cells display unpaired autosomal univalents and persistence of double strand breaks

• however, no signs of precocious separation of sister chromatids is detected

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

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