Analysis Tools
behavior/neurological
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• when tested for retention 24 hours after contextual fear conditioning, mutants show reduced levels of freezing relative to wild-type (mutant - 36.6% vs wild-type - 57.5%)
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• 48 hours after a cued fear conditioning test, where a tone was the conditioned stimulus, mutants show a low level of freezing prior to tone presentation, indicating impairment of contextual memory
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• in the Morris water maze test, mutants showed a tendency toward longer escape latencies compared to wild-type
• in probe trials with the platform hidden, mutant mice spend less time searching the target quadrant, and show less accurate identification of the precise platform location by having a reduced number of platform crossings
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nervous system
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• field EPSPs in mutant hippocampal slice preparations return to levels near unstimulated levels by 3 hours posttetaniziation, while in control slices the fEPSPs (ie. late LTP remains elevated
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• application of 4 trains of tetanic stimulation at 5 minute intervals, elicits long-lasting (>3 hours) potentiation in control hippocampal slices, but in mutants potentiation is unstable and decays progressively
• mutants display selective impairment in translational component of late-LTP (L-LTP); the defect is transcription-independent, translation-dependent, as shown by difference in inhibition kinetics of actinomycin-D (transcriptional inhibitor) and anisomycin (translational inhibitor)
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homeostasis/metabolism
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• ERK activation in the forebrain is inhibited, causing selective deficits in hippocampal memory retention and translation-dependent phase of late-LTP
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