Phenotypes associated with this allele

• Allele Symbol: Sphk1^tm2Cgh
• Allele Name: targeted mutation 2, Shaun R Coughlin
• Allele ID: MGI:3707997
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Lyve1^tm1.1(EGFP/cre)Cys/Lyve1^+ Sphk1^tm2Cgh/Sphk1^tm2Cgh Sphk2^tm1.1Cgh/Sphk2^tm1.1Cgh	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6	MGI:4421697
cn2	Sphk1^tm1.1Cgh/Sphk1^tm2Cgh Sphk2^tm1.1Cgh/Sphk2^tm1.1Cgh Tg(Mx1-cre)1Cgn/0	involves: 129S4/SvJae * C57BL/6 * CBA	MGI:3708981

Genotype
MGI:4421697

cn1

• Allelic Composition: Lyve1^{tm1.1(EGFP/cre)Cys}/Lyve1⁺; Sphk1^tm2Cgh/Sphk1^tm2Cgh; Sphk2^tm1.1Cgh/Sphk2^tm1.1Cgh
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Impaired lymphocyte egress in Lyve1^{tm1.1(EGFP/cre)Cys}/Lyve1⁺ Sphk1^tm2Cgh/Sphk1^tm2Cgh Sphk2^tm1.1Cgh/Sphk2^tm1.1Cgh mice

immune system

immune system phenotype ( J:156826 )

N • lymphatics of mutants can support bone marrow-derived dendritic cell migration

abnormal leukocyte migration ( J:156826 )

• egress of lymphocytes from lymph nodes is impaired

• lymphatics of mutants can support bone marrow-derived dendritic cell migration

abnormal lymphocyte cell number ( J:156826 )

• a decrease in lymphocyte numbers is detected in the circulation and spleen

decreased B cell number ( J:156826 )

• markedly reduced in lymph isolated from cisterna chyli

decreased T cell number ( J:156826 )

• markedly reduced in lymph isolated from cisterna chyli; reduction in T cells consistently seen in lymphocyte compartment of lymph nodes, but Peyer's patches show accumulation of naive T cells

decreased splenocyte number ( J:156826 )

• lymphocyte number is reduced in the spleen

abnormal Peyer's patch T cell area morphology ( J:156826 )

• accumulation of naive T cells in Peyer's patches is observed concomitant with reduced T cell numbers in the lymph nodes

abnormal lymph node morphology ( J:156826 )

• lymphatic sinuses show deficiency of lymphocytes

• Lyve1-positive sinuses appear collapsed compared to control sinuses, which are extended with cells

abnormal lymphatic vessel morphology ( J:156826 )

• lymphatic vessels in the trachea appear tortuous and ragged with occasional sprouts, in contrast to the smooth tubular structure in control animals; lymphatics within the diaphragm have similar altered morphology

• cell-cell junctions of initial lymphatics in the diaphragm are more poorly defined, being made up of fewer or more diffuse Cdh5 (VE-cadherin) +ve "buttons"

hematopoietic system

abnormal leukocyte migration ( J:156826 )

• egress of lymphocytes from lymph nodes is impaired

• lymphatics of mutants can support bone marrow-derived dendritic cell migration

abnormal lymphocyte cell number ( J:156826 )

• a decrease in lymphocyte numbers is detected in the circulation and spleen

decreased B cell number ( J:156826 )

• markedly reduced in lymph isolated from cisterna chyli

decreased T cell number ( J:156826 )

• markedly reduced in lymph isolated from cisterna chyli; reduction in T cells consistently seen in lymphocyte compartment of lymph nodes, but Peyer's patches show accumulation of naive T cells

decreased splenocyte number ( J:156826 )

• lymphocyte number is reduced in the spleen

cellular

abnormal leukocyte migration ( J:156826 )

• egress of lymphocytes from lymph nodes is impaired

• lymphatics of mutants can support bone marrow-derived dendritic cell migration

Genotype
MGI:3708981

cn2

• Allelic Composition: Sphk1^tm1.1Cgh/Sphk1^tm2Cgh; Sphk2^tm1.1Cgh/Sphk2^tm1.1Cgh; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * CBA

immune system

abnormal thymus cell ratio ( J:120783 )

• accumulation of mature T cells in the thymus without changes in other thymic subsets

abnormal NK cell differentiation ( J:154069 )

• mice exhibit a reduction in NK cell egression from the peripheral lymph nodes compared with wild-type mice

abnormal T cell differentiation ( J:120783 )

• accumulation of mature T cells in the thymus without changes in other thymic subsets

decreased B cell number ( J:120783 )

• blood and lymph B cell counts are significantly lower than controls

• however, the number of B cells in the spleen and lymph node is normal

decreased NK cell number ( J:154069 )

• in the spleen and lymph

increased NK cell number ( J:154069 )

• in bone marrow and slightly in the lymph nodes

decreased T cell number ( J:120783 )

• mice treated with polyinosine polycytidylic acid at P3 - 5 display markedly reduced T cell number in blood and lymph

• however, spleen and lymph node T cell numbers are normal

abnormal immune serum protein physiology ( J:120783 )

• undetectable levels of sphingosine-1-phosphate (S1P) in the plasma and lymph

hematopoietic system

abnormal thymus cell ratio ( J:120783 )

• accumulation of mature T cells in the thymus without changes in other thymic subsets

abnormal NK cell differentiation ( J:154069 )

• mice exhibit a reduction in NK cell egression from the peripheral lymph nodes compared with wild-type mice

abnormal T cell differentiation ( J:120783 )

• accumulation of mature T cells in the thymus without changes in other thymic subsets

decreased bone marrow cell number ( J:120783 )

• decrease in the number of mature B cells in the bone marrow

• however, immature cell numbers are similar to controls

decreased B cell number ( J:120783 )

• blood and lymph B cell counts are significantly lower than controls

• however, the number of B cells in the spleen and lymph node is normal

decreased NK cell number ( J:154069 )

• in the spleen and lymph

increased NK cell number ( J:154069 )

• in bone marrow and slightly in the lymph nodes

decreased T cell number ( J:120783 )

• mice treated with polyinosine polycytidylic acid at P3 - 5 display markedly reduced T cell number in blood and lymph

• however, spleen and lymph node T cell numbers are normal

endocrine/exocrine glands

abnormal thymus cell ratio ( J:120783 )

• accumulation of mature T cells in the thymus without changes in other thymic subsets

cardiovascular system

hemorrhage ( J:205423 )

• after ovalbumin and CFA challenge, pIpC-treated mice exhibit bleeding in the draining peripheral lymph nodes and increased permeability to FITC-dextran unlike control mice

• however, pIpC-treated mice do not exhibit spontaneous bleeding into mucosal lymph nodes

increased vascular permeability ( J:205423 )

• after ovalbumin and CFA challenge, pIpC-treated mice exhibit bleeding in the draining peripheral lymph nodes and increased permeability to FITC-dextran unlike control mice

• however, pIpC-treated mice do not exhibit spontaneous bleeding into mucosal lymph nodes and transfusion with wild-type platelets reduces vascular leakage of FITC-dextran