Phenotypes associated with this allele

• Allele Symbol: Tg(Camk2a-Bdnf)A9Stl
• Allele Name: transgene insertion A9, Susumu Tonegawa
• Allele ID: MGI:3709467
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
tg1	Tg(Camk2a-Bdnf)A9Stl/0	C57BL/6-Tg(Camk2a-Bdnf)A9Stl/J	MGI:5617753
tg2	Tg(Camk2a-Bdnf)A9Stl/0	involves: C57BL/6 * DBA/2	MGI:3709983

Genotype
MGI:5617753

tg1

• Allelic Composition: Tg(Camk2a-Bdnf)A9Stl/0
• Genetic Background: C57BL/6-Tg(Camk2a-Bdnf)A9Stl/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal behavior ( J:216846 )

♂ • mice bury less marbles than wild-type mice

♂ • however, mice show normal behavior in the social approach test and in the forced swim test

decreased anxiety-related response ( J:216846 )

♂ • mice spend more time in the open arms of the elevated plus-maze and make more entries to the open arms than wild-type mice

seizures ( J:216846 )

♂ • mice exhibit spontaneous seizures after 5 months of age, with 3 of 12 mice showing seizures at 9 months of age

♂ • seizure behavior includes head bobbing, forelimb and hindlimb clonus that progresses into running and jumping seizures followed by falling and the continuation of head bobbing and forelimb clonus

psychomotor seizures ( J:216846 )

♂ • seizures are similar to stage 5 limbic seizures

growth/size/body

decreased body weight ( J:216846 )

♂ • mice show decreased body weight that becomes more pronounced with age

nervous system

seizures ( J:216846 )

♂ • mice exhibit spontaneous seizures after 5 months of age, with 3 of 12 mice showing seizures at 9 months of age

♂ • seizure behavior includes head bobbing, forelimb and hindlimb clonus that progresses into running and jumping seizures followed by falling and the continuation of head bobbing and forelimb clonus

psychomotor seizures ( J:216846 )

♂ • seizures are similar to stage 5 limbic seizures

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
NOT	autism spectrum disorder	DOID:0060041		J:216846
epilepsy		DOID:1826		J:216846

Genotype
MGI:3709983

tg2

• Allelic Composition: Tg(Camk2a-Bdnf)A9Stl/0
• Genetic Background: involves: C57BL/6 * DBA/2

nervous system

nervous system phenotype ( J:57645 )

N • brain development at 4 weeks is grossly normal; cortex is of normal thickness, and lamination is intact with normal neuronal cell density in the visual cortex

abnormal neuron differentiation ( J:57645 )

• development of GABAergic neurons is accelerated in the primary visual cortex of transgenic mice between P12 and P35, relative to wild-type mice

abnormal brain interneuron morphology ( J:57645 )

• at P17, number of Parvalbumin-positive interneurons in the visual cortex is ~double the number found in wild-type brains

• these interneurons have larger soma sizes and more extensive neurite arborizations than in wild-type at P17

abnormal neocortex morphology ( J:57645 )

• at 5 weeks, BDNF mRNA levels in neocortex of transgenic mice are ~3-fold higher than in wild-type littermates

abnormal visual cortex morphology ( J:57645 )

• ocular dominance (OD) plasticity ends prematurely in transgenic mice relative to wild-type; the OD shift induced by 4-day monocular deprivation (MD) is blocked when done at P28, near the peak of OD shift in wild-type

• shifts in the contralateral/ipsilateral visual-evoked potential (C/I VEP) ratios are larger in transgenics when MD is done with a difference of ~1 week compared to wild-type

abnormal inhibitory postsynaptic currents ( J:57645 )

• in layer III pyramidal neurons in hippoccampal slices, amplitude of the maximal IPSC (462 pA) is consistently large than in wild-type (279 pA)

• stimulus intensity required to recruit a saturating IPSC is similar in wild-type and transgenic brain slices

• EPSC amplitudes and whole-cell capacitances are similar between transgenic and wild-type mice

reduced long-term potentiation ( J:57645 )

• theta burst stimulation (TBS) at the white matter-layer IV border (WM->III) induces little LTP in transgenic mice (101%), compared to the robust LTP induced in wild-type mice (122%)

• WM->III LTP undergoes a sharp decline between 3 and 4 weeks postnatal, which is 1 week earlier than observed in wild-type littermates (between 4 and 5 weeks of age)

• when inhibition is induced in cortical slices from transgenic mice with picrotoxin application, WM->III LTP can be supported by mice at P23-P25

vision/eye

abnormal eye physiology ( J:57645 )

• visual acuity is accelerated in transgenic mice relative to wild-type; visual acuity reaches adult levels by P24-25, a week sooner than in wild-type

cellular

abnormal neuron differentiation ( J:57645 )

• development of GABAergic neurons is accelerated in the primary visual cortex of transgenic mice between P12 and P35, relative to wild-type mice