Phenotypes associated with this allele

• Allele Symbol: B4gat1^tm1Mifu
• Allele Name: targeted mutation 1, Minoru Fukuda
• Allele ID: MGI:3710015
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	B4gat1^tm1Mifu/B4gat1^tm1Mifu	B6.Cg-B4gat1^tm1Mifu	MGI:3711067
cx2	B4gat1^tm1Mifu/B4gat1^tm1Mifu Gcnt1^tm1Jxm/Gcnt1^tm1Jxm	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3711068

Genotype
MGI:3711067

hm1

• Allelic Composition: B4gat1^tm1Mifu/B4gat1^tm1Mifu
• Genetic Background: B6.Cg-B4gat1^tm1Mifu

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:120722 )

N • dinitrofluorobenzene-induced contact hypersensitivity is similar to wild-type mice unlike in mice double homozygous for this allele and Gcnt1^tm1Jxm

impaired leukocyte tethering or rolling ( J:120722 )

• increase in rolling velocities and reduction in sticking of B and T cells

• increase in rolling and reduction in sticking is more severe in B cells than in T cells

abnormal lymph node cell ratio ( J:120722 )

• however, the total number of lymphocytes in secondary lymphoid organs is similar to wild-type

• fewer B lymphocytes are found in the lymph node and this is accompa nied by an increase in the number of T cells present

cellular

impaired leukocyte tethering or rolling ( J:120722 )

• increase in rolling velocities and reduction in sticking of B and T cells

• increase in rolling and reduction in sticking is more severe in B cells than in T cells

hematopoietic system

impaired leukocyte tethering or rolling ( J:120722 )

• increase in rolling velocities and reduction in sticking of B and T cells

• increase in rolling and reduction in sticking is more severe in B cells than in T cells

Genotype
MGI:3711068

cx2

• Allelic Composition: B4gat1^tm1Mifu/B4gat1^tm1Mifu; Gcnt1^tm1Jxm/Gcnt1^tm1Jxm
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

immune system

abnormal leukocyte migration ( J:120722 )

• homing is 45% and 65% of wild-type mice in 2-hour and 20-hour assays, respectively, and is not inhibited by MECA-79 unlike in wild-type mice

• after challenge with dinitrofluorobenzene homing of lymphocytes to draining lymph nodes and lymphocyte numbers are reduced by 37% compared to wild-type mice

impaired leukocyte tethering or rolling ( J:120722 )

• rolling velocities of T lymphocytes in venules (orders III and IV) are increased about 3-fold

• the percentage of cells rolling and sticking is decreased

abnormal lymph node cell ratio ( J:120722 )

• more severe decrease in the number of lymph-node resident B cells compared to single homozygotes

• however, no change is detected in the total number of lymphocytes found in secondary lymphoid tissues

decreased susceptibility to type IV hypersensitivity reaction ( J:120722 )

• when sensitized and challenged with dinitrofluorobenzee (DNFB) mice exhibit 37% less ear swelling and have less CD3+ T lymphocyte infiltration than wild-type mice

cellular

abnormal leukocyte migration ( J:120722 )

• homing is 45% and 65% of wild-type mice in 2-hour and 20-hour assays, respectively, and is not inhibited by MECA-79 unlike in wild-type mice

• after challenge with dinitrofluorobenzene homing of lymphocytes to draining lymph nodes and lymphocyte numbers are reduced by 37% compared to wild-type mice

impaired leukocyte tethering or rolling ( J:120722 )

• rolling velocities of T lymphocytes in venules (orders III and IV) are increased about 3-fold

• the percentage of cells rolling and sticking is decreased

hematopoietic system

abnormal leukocyte migration ( J:120722 )

• homing is 45% and 65% of wild-type mice in 2-hour and 20-hour assays, respectively, and is not inhibited by MECA-79 unlike in wild-type mice

• after challenge with dinitrofluorobenzene homing of lymphocytes to draining lymph nodes and lymphocyte numbers are reduced by 37% compared to wild-type mice

impaired leukocyte tethering or rolling ( J:120722 )

• rolling velocities of T lymphocytes in venules (orders III and IV) are increased about 3-fold

• the percentage of cells rolling and sticking is decreased