About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Ncaph2nes
nessy
MGI:3710093
Summary 3 genotypes


Genotype
MGI:6194677
hm1
Allelic
Composition		 Ncaph2nes/Ncaph2nes
Genetic
Background		 C57BL/6-Ncaph2nes
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ncaph2nes mutation (3 available); any Ncaph2 mutation (38 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal mitosis ( J:242515 )
• an increase in the frequency of chromatin bridge formation during anaphase is seen in apical neural progenitors during neurogenesis at E13.5 indicating that chromosome segregation is impaired
• mouse embryonic fibroblasts exhibit higher levels of chromosome segregation defects
• mice exhibit an increase in frequency of micronucleated reticulocytes and mature erythrocytes due to segregation errors
• however, mitotic spindle position in neuroepithelia is normal

growth/size/body
decreased body weight ( J:242515 )
microcephaly ( J:242515 )

nervous system
decreased brain weight ( J:242515 )
• reduction in brain weight at 8 weeks of age
abnormal cerebral cortex morphology ( J:242515 )
• reduction in cortical surface area at 8 weeks of age
abnormal neocortex size ( J:242515 )
• a 4% reduction in neocortical thickness is seen at E13.5
• however, relative thickness of the ventricular zone, subventricular zone, and the neuronal preplate layer are unchanged




Genotype
MGI:3718589
hm2
Allelic
Composition		 Ncaph2nes/Ncaph2nes
Genetic
Background		 C57BL/6JSfdAnu-Ncaph2nes/Anu
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ncaph2nes mutation (3 available); any Ncaph2 mutation (38 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal thymus cell ratio ( J:123300 )
• increase in the proportion of double negative T cell progenitors with high levels of CD44 expression
abnormal thymus cortex morphology ( J:123300 )
• sparse cortical cells
abnormal thymus corticomedullary boundary morphology ( J:123300 )
• poorly defined corticomedullary junctions
thymus hypoplasia ( J:123300 )
• smaller thymus with a 10-fold reduction in thymocyte numbers
abnormal T cell morphology ( J:123300 )
• in cultures from bone marrow, DN4 cell proliferation is significantly reduced
• however, proliferation of DN3 cells is similar to controls
• despite T cell defects, no difference in B cell populations is seen compared to controls
abnormal double-negative T cell morphology ( J:123300 )
• increase in the proportion of double negative T cell progenitors with high levels of CD44 expression
decreased T cell number ( J:123300 )
• decrease of about 2.7-fold in spleen T cell numbers
• 50- to 100-fold reduction in single positive cell numbers in the thymus
decreased thymocyte number ( J:123300 )
• 10-fold reduction in thymocyte numbers
decreased double-negative T cell number ( J:123300 )
• 5-fold reduction in DN4 cells in the thymus
decreased double-positive T cell number ( J:123300 )
• 100-fold reduction in double positive cell numbers in the thymus
decreased CD8-positive, alpha-beta T cell number ( J:123300 )
• loss of the main subset of naive CD8+ T cells with low levels of CD44 expression results in elevated mean CD44 expression of blood CD8+ T cells
abnormal splenic cell ratio ( J:123300 )
• decrease of about 2.7-fold in spleen T cell numbers

hematopoietic system
abnormal thymus cell ratio ( J:123300 )
• increase in the proportion of double negative T cell progenitors with high levels of CD44 expression
abnormal thymus cortex morphology ( J:123300 )
• sparse cortical cells
abnormal thymus corticomedullary boundary morphology ( J:123300 )
• poorly defined corticomedullary junctions
thymus hypoplasia ( J:123300 )
• smaller thymus with a 10-fold reduction in thymocyte numbers
abnormal T cell morphology ( J:123300 )
• in cultures from bone marrow, DN4 cell proliferation is significantly reduced
• however, proliferation of DN3 cells is similar to controls
• despite T cell defects, no difference in B cell populations is seen compared to controls
abnormal double-negative T cell morphology ( J:123300 )
• increase in the proportion of double negative T cell progenitors with high levels of CD44 expression
decreased T cell number ( J:123300 )
• decrease of about 2.7-fold in spleen T cell numbers
• 50- to 100-fold reduction in single positive cell numbers in the thymus
decreased thymocyte number ( J:123300 )
• 10-fold reduction in thymocyte numbers
decreased double-negative T cell number ( J:123300 )
• 5-fold reduction in DN4 cells in the thymus
decreased double-positive T cell number ( J:123300 )
• 100-fold reduction in double positive cell numbers in the thymus
decreased CD8-positive, alpha-beta T cell number ( J:123300 )
• loss of the main subset of naive CD8+ T cells with low levels of CD44 expression results in elevated mean CD44 expression of blood CD8+ T cells
abnormal splenic cell ratio ( J:123300 )
• decrease of about 2.7-fold in spleen T cell numbers

endocrine/exocrine glands
abnormal thymus cell ratio ( J:123300 )
• increase in the proportion of double negative T cell progenitors with high levels of CD44 expression
abnormal thymus cortex morphology ( J:123300 )
• sparse cortical cells
abnormal thymus corticomedullary boundary morphology ( J:123300 )
• poorly defined corticomedullary junctions
thymus hypoplasia ( J:123300 )
• smaller thymus with a 10-fold reduction in thymocyte numbers
decreased thymocyte number ( J:123300 )
• 10-fold reduction in thymocyte numbers




Genotype
MGI:3718590
cx3
Allelic
Composition		 Ncaph2nes/Ncaph2nes
Tg(TcrHEL3A9)1Mmd/0
Genetic
Background		 C57BL/6-Ncaph2nes Tg(TcrHEL3A9)1Mmd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ncaph2nes mutation (3 available); any Ncaph2 mutation (38 available)
Tg(TcrHEL3A9)1Mmd mutation (7 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal thymus cell ratio ( J:123300 )
• decrease in the proportion of double positive cells and increase in the proportion of DN4 cells
abnormal double-negative T cell morphology ( J:123300 )
• increase in the proportion of double negative T cell progenitors with high levels of CD44 expression in the thymus
decreased double-positive T cell number ( J:123300 )
• decrease in the proportion of double positive cells in the thymus similar to homozygous mice not expressing the prearranged T cell receptor transgene

hematopoietic system
abnormal thymus cell ratio ( J:123300 )
• decrease in the proportion of double positive cells and increase in the proportion of DN4 cells
abnormal double-negative T cell morphology ( J:123300 )
• increase in the proportion of double negative T cell progenitors with high levels of CD44 expression in the thymus
decreased double-positive T cell number ( J:123300 )
• decrease in the proportion of double positive cells in the thymus similar to homozygous mice not expressing the prearranged T cell receptor transgene

endocrine/exocrine glands
abnormal thymus cell ratio ( J:123300 )
• decrease in the proportion of double positive cells and increase in the proportion of DN4 cells




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
08/02/2024
MGI 6.24 		The Jackson Laboratory