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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Ncaph2nes
nessy
MGI:3710093
Summary 3 genotypes


Genotype
MGI:6194677
hm1
Allelic
Composition		 Ncaph2nes/Ncaph2nes
Genetic
Background		 C57BL/6-Ncaph2nes
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ncaph2nes mutation (3 available); any Ncaph2 mutation (38 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal mitosis ( J:242515 )
• an increase in the frequency of chromatin bridge formation during anaphase is seen in apical neural progenitors during neurogenesis at E13.5 indicating that chromosome segregation is impaired
• mouse embryonic fibroblasts exhibit higher levels of chromosome segregation defects
• mice exhibit an increase in frequency of micronucleated reticulocytes and mature erythrocytes due to segregation errors
• however, mitotic spindle position in neuroepithelia is normal

growth/size/body
decreased body weight ( J:242515 )
microcephaly ( J:242515 )

nervous system
decreased brain weight ( J:242515 )
• reduction in brain weight at 8 weeks of age
abnormal cerebral cortex morphology ( J:242515 )
• reduction in cortical surface area at 8 weeks of age
abnormal neocortex size ( J:242515 )
• a 4% reduction in neocortical thickness is seen at E13.5
• however, relative thickness of the ventricular zone, subventricular zone, and the neuronal preplate layer are unchanged




Genotype
MGI:3718589
hm2
Allelic
Composition		 Ncaph2nes/Ncaph2nes
Genetic
Background		 C57BL/6JSfdAnu-Ncaph2nes/Anu
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ncaph2nes mutation (3 available); any Ncaph2 mutation (38 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal thymus cell ratio ( J:123300 )
• increase in the proportion of double negative T cell progenitors with high levels of CD44 expression
abnormal thymus cortex morphology ( J:123300 )
• sparse cortical cells
abnormal thymus corticomedullary boundary morphology ( J:123300 )
• poorly defined corticomedullary junctions
thymus hypoplasia ( J:123300 )
• smaller thymus with a 10-fold reduction in thymocyte numbers
abnormal T cell morphology ( J:123300 )
• in cultures from bone marrow, DN4 cell proliferation is significantly reduced
• however, proliferation of DN3 cells is similar to controls
• despite T cell defects, no difference in B cell populations is seen compared to controls
abnormal double-negative T cell morphology ( J:123300 )
• increase in the proportion of double negative T cell progenitors with high levels of CD44 expression
decreased T cell number ( J:123300 )
• decrease of about 2.7-fold in spleen T cell numbers
• 50- to 100-fold reduction in single positive cell numbers in the thymus
decreased thymocyte number ( J:123300 )
• 10-fold reduction in thymocyte numbers
decreased double-negative T cell number ( J:123300 )
• 5-fold reduction in DN4 cells in the thymus
decreased double-positive T cell number ( J:123300 )
• 100-fold reduction in double positive cell numbers in the thymus
decreased CD8-positive, alpha-beta T cell number ( J:123300 )
• loss of the main subset of naive CD8+ T cells with low levels of CD44 expression results in elevated mean CD44 expression of blood CD8+ T cells
abnormal splenic cell ratio ( J:123300 )
• decrease of about 2.7-fold in spleen T cell numbers

hematopoietic system
abnormal thymus cell ratio ( J:123300 )
• increase in the proportion of double negative T cell progenitors with high levels of CD44 expression
abnormal thymus cortex morphology ( J:123300 )
• sparse cortical cells
abnormal thymus corticomedullary boundary morphology ( J:123300 )
• poorly defined corticomedullary junctions
thymus hypoplasia ( J:123300 )
• smaller thymus with a 10-fold reduction in thymocyte numbers
abnormal T cell morphology ( J:123300 )
• in cultures from bone marrow, DN4 cell proliferation is significantly reduced
• however, proliferation of DN3 cells is similar to controls
• despite T cell defects, no difference in B cell populations is seen compared to controls
abnormal double-negative T cell morphology ( J:123300 )
• increase in the proportion of double negative T cell progenitors with high levels of CD44 expression
decreased T cell number ( J:123300 )
• decrease of about 2.7-fold in spleen T cell numbers
• 50- to 100-fold reduction in single positive cell numbers in the thymus
decreased thymocyte number ( J:123300 )
• 10-fold reduction in thymocyte numbers
decreased double-negative T cell number ( J:123300 )
• 5-fold reduction in DN4 cells in the thymus
decreased double-positive T cell number ( J:123300 )
• 100-fold reduction in double positive cell numbers in the thymus
decreased CD8-positive, alpha-beta T cell number ( J:123300 )
• loss of the main subset of naive CD8+ T cells with low levels of CD44 expression results in elevated mean CD44 expression of blood CD8+ T cells
abnormal splenic cell ratio ( J:123300 )
• decrease of about 2.7-fold in spleen T cell numbers

endocrine/exocrine glands
abnormal thymus cell ratio ( J:123300 )
• increase in the proportion of double negative T cell progenitors with high levels of CD44 expression
abnormal thymus cortex morphology ( J:123300 )
• sparse cortical cells
abnormal thymus corticomedullary boundary morphology ( J:123300 )
• poorly defined corticomedullary junctions
thymus hypoplasia ( J:123300 )
• smaller thymus with a 10-fold reduction in thymocyte numbers
decreased thymocyte number ( J:123300 )
• 10-fold reduction in thymocyte numbers




Genotype
MGI:3718590
cx3
Allelic
Composition		 Ncaph2nes/Ncaph2nes
Tg(TcrHEL3A9)1Mmd/0
Genetic
Background		 C57BL/6-Ncaph2nes Tg(TcrHEL3A9)1Mmd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ncaph2nes mutation (3 available); any Ncaph2 mutation (38 available)
Tg(TcrHEL3A9)1Mmd mutation (6 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal thymus cell ratio ( J:123300 )
• decrease in the proportion of double positive cells and increase in the proportion of DN4 cells
abnormal double-negative T cell morphology ( J:123300 )
• increase in the proportion of double negative T cell progenitors with high levels of CD44 expression in the thymus
decreased double-positive T cell number ( J:123300 )
• decrease in the proportion of double positive cells in the thymus similar to homozygous mice not expressing the prearranged T cell receptor transgene

hematopoietic system
abnormal thymus cell ratio ( J:123300 )
• decrease in the proportion of double positive cells and increase in the proportion of DN4 cells
abnormal double-negative T cell morphology ( J:123300 )
• increase in the proportion of double negative T cell progenitors with high levels of CD44 expression in the thymus
decreased double-positive T cell number ( J:123300 )
• decrease in the proportion of double positive cells in the thymus similar to homozygous mice not expressing the prearranged T cell receptor transgene

endocrine/exocrine glands
abnormal thymus cell ratio ( J:123300 )
• decrease in the proportion of double positive cells and increase in the proportion of DN4 cells




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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory