Phenotypes associated with this allele

• Allele Symbol: Taf7l^tm1.1Jw
• Allele Name: targeted mutation 1.1, Jeremy Wang
• Allele ID: MGI:3710399
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Taf7l^tm1.1Jw/Taf7l^tm1.1Jw	involves: 129S4/SvJae * BALB/c * C57BL/6 * FVB/N	MGI:3710401
ot2	Taf7l^tm1.1Jw/Y	129X1.Cg-Taf7l^tm1.1Jw	MGI:3710418
ot3	Taf7l^tm1.1Jw/Y	B6J.Cg-Taf7l^tm1.1Jw	MGI:3710415
ot4	Taf7l^tm1.1Jw/Y	involves: 129S4/SvJae * BALB/c * C57BL/6 * FVB/N	MGI:3710402

Genotype
MGI:3710401

hm1

• Allelic Composition: Taf7l^tm1.1Jw/Taf7l^tm1.1Jw
• Genetic Background: involves: 129S4/SvJae * BALB/c * C57BL/6 * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:121371 )

♀N • mice are fertile and have normal litter sizes

Genotype
MGI:3710418

ot2

• Allelic Composition: Taf7l^tm1.1Jw/Y
• Genetic Background: 129X1.Cg-Taf7l^tm1.1Jw

reproductive system

asthenozoospermia ( J:121371 )

♂ • percentage of motile sperm is reduced (56.3+/-2.1% compared to 72.7+/-4.2% in wild-type), all three velocity parameters are significantly lower, and while sperm beat more rapidly they fail to produce motion

♂ • sperm motility is further reduced from 56.3% to 45.5% upon capacitation

abnormal testis morphology ( J:121371 )

♂

abnormal seminiferous tubule morphology ( J:121371 )

♂ • in testes of mice older than 7 months, at least one large vacuole develops in 15.2+/-4.6% of tubules

decreased testis weight ( J:121371 )

♂ • at 8 weeks, testes weigh 10% less than in wild-type mice

abnormal gametogenesis ( J:121371 )

♂

oligozoospermia ( J:121371 )

♂ • sperm counts are reduced by greater than 50%

teratozoospermia ( J:121371 )

♂ • at 8 weeks, 46% of sperm tails are folded or angulated compared to 5.8% in wild-type

♂ • cytoplasmic membrane is retracted from the region of folding

hairpin sperm flagellum ( J:121371 )

♂ • sperm tails are frequently bent by 180 within the middle piece or at the junction between the middle and principal pieces

kinked sperm flagellum ( J:121371 )

♂ • sharp angulation of sperm tails at the junction of the middle and principal pieces is frequently observed

decreased litter size ( J:121371 )

♂ • Background Sensitivity: unlike mice on a mixed background, males congenic on a 129 background produce smaller litter sizes of 3.8+/-2.1 pups compared to 8.3+/-2.5 pups in wild-type

reduced male fertility ( J:121371 )

♂

endocrine/exocrine glands

abnormal testis morphology ( J:121371 )

♂

abnormal seminiferous tubule morphology ( J:121371 )

♂ • in testes of mice older than 7 months, at least one large vacuole develops in 15.2+/-4.6% of tubules

decreased testis weight ( J:121371 )

♂ • at 8 weeks, testes weigh 10% less than in wild-type mice

cellular

oligozoospermia ( J:121371 )

♂ • sperm counts are reduced by greater than 50%

teratozoospermia ( J:121371 )

♂ • at 8 weeks, 46% of sperm tails are folded or angulated compared to 5.8% in wild-type

♂ • cytoplasmic membrane is retracted from the region of folding

hairpin sperm flagellum ( J:121371 )

♂ • sperm tails are frequently bent by 180 within the middle piece or at the junction between the middle and principal pieces

kinked sperm flagellum ( J:121371 )

♂ • sharp angulation of sperm tails at the junction of the middle and principal pieces is frequently observed

asthenozoospermia ( J:121371 )

♂ • percentage of motile sperm is reduced (56.3+/-2.1% compared to 72.7+/-4.2% in wild-type), all three velocity parameters are significantly lower, and while sperm beat more rapidly they fail to produce motion

♂ • sperm motility is further reduced from 56.3% to 45.5% upon capacitation

Genotype
MGI:3710415

ot3

• Allelic Composition: Taf7l^tm1.1Jw/Y
• Genetic Background: B6J.Cg-Taf7l^tm1.1Jw

reproductive system

asthenozoospermia ( J:121371 )

♂ • percentage of motile sperm is reduced (56.3+/-2.1% compared to 72.7+/-4.2% in wild-type), all three velocity parameters are significantly lower, and while sperm beat more rapidly they fail to produce motion

♂ • sperm motility is further reduced from 56.3% to 45.5% upon capacitation

abnormal testis morphology ( J:121371 )

♂

abnormal seminiferous tubule morphology ( J:121371 )

♂ • in testes of mice older than 7 months, at least one large vacuole develops in 15.2+/-4.6% of tubules

decreased testis weight ( J:121371 )

♂ • at 8 weeks, testes weigh 10% less than in wild-type mice

abnormal gametogenesis ( J:121371 )

♂

oligozoospermia ( J:121371 )

♂ • sperm counts are reduced by greater than 50%

teratozoospermia ( J:121371 )

♂ • at 8 weeks, 46% of sperm tails are folded or angulated compared to 5.8% in wild-type

♂ • cytoplasmic membrane is retracted from the region of folding

hairpin sperm flagellum ( J:121371 )

♂ • sperm tails are frequently bent by 180 within the middle piece or at the junction between the middle and principal pieces

kinked sperm flagellum ( J:121371 )

♂ • sharp angulation of sperm tails at the junction of the middle and principal pieces is frequently observed

decreased litter size ( J:121371 )

♂ • Background Sensitivity: unlike mice on a mixed background, males congenic on a C57BL/6 background produce smaller litter sizes of 3.8+/-2.1 pups compared to 8.3+/-2.5 pups in wild-type

reduced male fertility ( J:121371 )

♂

adipose tissue

abnormal brown adipose tissue morphology ( J:228546 )

♂ • at E18.5, interscapular UCP1-positive BAT is largely disorganized, significantly reduced in size, and contains decreased lipid levels

♂ • MYHC (myosin heavy chain) staining of E18.5 embryos revealed skeletal muscle-tissue structures emerging and invading regions that normally contain BAT, unlike in wild-type embryos

decreased brown adipose tissue amount ( J:228546 )

♂ • at E18.5, interscapular UCP1-positive BAT is significantly reduced in size

♂ • whole BAT is reduced at 1 and 4 months of age

abnormal brown fat cell differentiation ( J:228546 )

♂ • BAT-selective genes involved in brown fat cell differentiation are downregulated by >3-fold in BAT tissue

♂ • in vitro, 5 days post-induction of BAT differentiation, isolated brown adipose progenitor cells form elongated muscle-like cells rather than round fat laden adipocytes, indicating a switch from a BAT cell fate to muscle lineages

decreased brown fat lipid droplet number ( J:228546 )

♂ • at E18.5, interscapular UCP1-positive BAT contains less lipid droplets

decreased interscapular fat pad weight ( J:228546 )

♂ • ~40% weight reduction of interscapular BAT at E18.5

abnormal brown adipose tissue thermogenesis ( J:228546 )

♂ • BAT-selective genes involved in brown fat thermogenesis are downregulated by >3-fold in BAT tissue

endocrine/exocrine glands

abnormal testis morphology ( J:121371 )

♂

abnormal seminiferous tubule morphology ( J:121371 )

♂ • in testes of mice older than 7 months, at least one large vacuole develops in 15.2+/-4.6% of tubules

decreased testis weight ( J:121371 )

♂ • at 8 weeks, testes weigh 10% less than in wild-type mice

cellular

abnormal brown fat cell differentiation ( J:228546 )

♂ • BAT-selective genes involved in brown fat cell differentiation are downregulated by >3-fold in BAT tissue

♂ • in vitro, 5 days post-induction of BAT differentiation, isolated brown adipose progenitor cells form elongated muscle-like cells rather than round fat laden adipocytes, indicating a switch from a BAT cell fate to muscle lineages

oligozoospermia ( J:121371 )

♂ • sperm counts are reduced by greater than 50%

teratozoospermia ( J:121371 )

♂ • at 8 weeks, 46% of sperm tails are folded or angulated compared to 5.8% in wild-type

♂ • cytoplasmic membrane is retracted from the region of folding

hairpin sperm flagellum ( J:121371 )

♂ • sperm tails are frequently bent by 180 within the middle piece or at the junction between the middle and principal pieces

kinked sperm flagellum ( J:121371 )

♂ • sharp angulation of sperm tails at the junction of the middle and principal pieces is frequently observed

asthenozoospermia ( J:121371 )

♂ • percentage of motile sperm is reduced (56.3+/-2.1% compared to 72.7+/-4.2% in wild-type), all three velocity parameters are significantly lower, and while sperm beat more rapidly they fail to produce motion

♂ • sperm motility is further reduced from 56.3% to 45.5% upon capacitation

abnormal mitochondrial physiology ( J:228546 )

♂ • BAT-selective genes involved in mitochondrial function are downregulated by >3-fold in BAT tissue

muscle

abnormal muscle development ( J:228546 )

♂ • muscle-specific genes involved in skeletal muscle development are upregulated by >3-fold in BAT tissue, consistent with enhanced skeletal muscle formation

abnormal muscle physiology ( J:228546 )

♂ • muscle-specific genes involved in skeletal muscle function are upregulated by >3-fold in BAT tissue

Genotype
MGI:3710402

ot4

• Allelic Composition: Taf7l^tm1.1Jw/Y
• Genetic Background: involves: 129S4/SvJae * BALB/c * C57BL/6 * FVB/N

reproductive system

reproductive system phenotype ( J:121371 )

♂N • Background Sensitivity: unlike mice on a congenic 129 or C57BL/6 background, mice on a mixed background are fertile with normal sized litters