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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Braftm1.1Wds
targeted mutation 1.1, William D Snider
MGI:3711007
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Braftm1.1Wds/Braftm1.1Wds involves: 129P2/OlaHsd * C57BL/6 MGI:3711453
cn2
 		Braftm1Wds/Braftm1.1Wds
Raf1tm1Bacc/Raf1tm2Bacc
Tg(Nes-cre)1Kln/0 		involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL MGI:3713654
cn3
 		Braftm1Wds/Braftm1.1Wds
Tg(Nes-cre)1Kln/0 		involves: 129P2/OlaHsd * C57BL/6 * SJL MGI:3713535


Genotype
MGI:3711453
hm1
Allelic
Composition		 Braftm1.1Wds/Braftm1.1Wds
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1.1Wds mutation (0 available); any Braf mutation (60 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging

embryo
embryonic growth retardation ( J:121660 )
• embryos show obvious growth retardation prior to death

growth/size/body
embryonic growth retardation ( J:121660 )
• embryos show obvious growth retardation prior to death

nervous system
increased neuron apoptosis ( J:121660 )
• ~90% of cultured DRG neurons from E12.5 embryos die within 5 days in presence of nerve growth factor compared to 19% loss of heterozygous control neurons

cellular
increased neuron apoptosis ( J:121660 )
• ~90% of cultured DRG neurons from E12.5 embryos die within 5 days in presence of nerve growth factor compared to 19% loss of heterozygous control neurons




Genotype
MGI:3713654
cn2
Allelic
Composition		 Braftm1Wds/Braftm1.1Wds
Raf1tm1Bacc/Raf1tm2Bacc
Tg(Nes-cre)1Kln/0
Genetic
Background		 involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1.1Wds mutation (0 available); any Braf mutation (60 available)
Braftm1Wds mutation (0 available); any Braf mutation (60 available)
Raf1tm1Bacc mutation (0 available); any Raf1 mutation (117 available)
Raf1tm2Bacc mutation (2 available); any Raf1 mutation (117 available)
Tg(Nes-cre)1Kln mutation (4 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
embryonic lethality during organogenesis, complete penetrance ( J:121660 )
• no double conditional embryos are found live at E14-15, but can be isolated at E13

nervous system
abnormal axon extension ( J:121660 )
• DRG neurons cultured with NGF for 5 days show impaired axon outgrowth
abnormal innervation ( J:121660 )
• sensory nerve trunks form normally, but distal arborization is reduced compared to controls
abnormal dorsal root ganglion morphology ( J:121660 )
• at E13, Ret levels in DRGs are reduced

cellular
abnormal axon extension ( J:121660 )
• DRG neurons cultured with NGF for 5 days show impaired axon outgrowth




Genotype
MGI:3713535
cn3
Allelic
Composition		 Braftm1Wds/Braftm1.1Wds
Tg(Nes-cre)1Kln/0
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Braftm1.1Wds mutation (0 available); any Braf mutation (60 available)
Braftm1Wds mutation (0 available); any Braf mutation (60 available)
Tg(Nes-cre)1Kln mutation (4 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
lethality at weaning, incomplete penetrance ( J:121660 )
• mice tend to die in third week postnatal, although some survive to P35

growth/size/body
decreased body weight ( J:121660 )
• at P32, body weight is ~25% of littermates
postnatal growth retardation ( J:121660 )
• from P12-14 onwards, mice show severe growth retardation

endocrine/exocrine glands
abnormal adenohypophysis morphology ( J:121660 )
• perivascular hypothalamic axonal innervation to anterior lobe is reduced
small adenohypophysis ( J:121660 )
• anterior lobe of pituitary gland is markedly reduced in size compared to controls

behavior/neurological
behavior/neurological phenotype ( J:121660 )
N
• mice show normal nociceptive response in hotplate assay
hyperactivity ( J:121660 )
• mice appear hyperactive

nervous system
nervous system phenotype ( J:121660 )
N
• dorsal root ganglion neurons survive in mutants; lumbar-vertebral neuron counts at L4 and L5 are similar to wild-type
abnormal adenohypophysis morphology ( J:121660 )
• perivascular hypothalamic axonal innervation to anterior lobe is reduced
small adenohypophysis ( J:121660 )
• anterior lobe of pituitary gland is markedly reduced in size compared to controls
thin cerebral cortex ( J:121660 )
• disproportionate thinning of cortex is observed

homeostasis/metabolism
hypoglycemia ( J:121660 )
• mice display hypoglycemia with blood glucose levels ~70% below normal
abnormal body temperature homeostasis ( J:121660 )
• when separated from other mice, body temperature drops 5 degrees within 15 minutes
abnormal growth hormone level ( J:121660 )
• level is elevated in pituitary relative to controls
• level of growth hormone is elevated in individual pituitary endocrine cells




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory