Phenotypes associated with this allele

• Allele Symbol: Trbv13-2^tm1(CD2)Zhu
• Allele Name: targeted mutation 1, Yuan Zhuang
• Allele ID: MGI:3711047
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Trbv13-2^tm1(CD2)Zhu/Trbv13-2^tm1(CD2)Zhu	involves: 129 * C57BL/6	MGI:3711856
hm2	Trbv13-2^tm1(CD2)Zhu/Trbv13-2^tm1(CD2)Zhu	involves: 129 * C57BL/6 * SJL	MGI:3711857
cx3	Tcrb^tm2Slek/Tcrb^tm2Slek Trbv13-2^tm1(CD2)Zhu/Trbv13-2^tm1(CD2)Zhu	involves: 129 * C57BL/6	MGI:3711858

Genotype
MGI:3711856

hm1

• Allelic Composition: Trbv13-2^tm1(CD2)Zhu/Trbv13-2^tm1(CD2)Zhu
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal T cell differentiation ( J:121608 )

• human CD2 is expressed by T cells at various stages of development in the thymus

• a fraction of gamma/delta T cells in thymus, spleen, and gut epithelium express human CD2; a small number of double negative (DN) stage 1 thymocytes (earliest T-cell progenitors) display human CD2 expression

• Background Sensitivity: bi-allelic activation of Tcrb-V8.2 is observed prior to allelic exclusion, with entire double negative stage 2/stage 3 compartment expressing the human CD2 marker, compared to half this expression level in heterozygous mice or B6/SJL F1 mice; this is independent of D-J and V-DJ rearrangement

• SP and DP (single- and double-positive) thymocytes from homozygous and heterozygous mice show a constant level of expression, indicating after V-DJ rearrangement, expression is monoallelic

immune system

immune system phenotype ( J:121608 )

N • thymocyte development is normal in knock-in mice, with total cell numbers, ratios passing through each developmental stage, and numbers of B and T cells in peripheral lymphoid organs similar to wild-type mice

N • no significant differences in Vbeta repertoire are observed relative to wild-type

abnormal T cell differentiation ( J:121608 )

• human CD2 is expressed by T cells at various stages of development in the thymus

• a fraction of gamma/delta T cells in thymus, spleen, and gut epithelium express human CD2; a small number of double negative (DN) stage 1 thymocytes (earliest T-cell progenitors) display human CD2 expression

• Background Sensitivity: bi-allelic activation of Tcrb-V8.2 is observed prior to allelic exclusion, with entire double negative stage 2/stage 3 compartment expressing the human CD2 marker, compared to half this expression level in heterozygous mice or B6/SJL F1 mice; this is independent of D-J and V-DJ rearrangement

• SP and DP (single- and double-positive) thymocytes from homozygous and heterozygous mice show a constant level of expression, indicating after V-DJ rearrangement, expression is monoallelic

Genotype
MGI:3711857

hm2

• Allelic Composition: Trbv13-2^tm1(CD2)Zhu/Trbv13-2^tm1(CD2)Zhu
• Genetic Background: involves: 129 * C57BL/6 * SJL

hematopoietic system

abnormal T cell differentiation ( J:121608 )

• Background Sensitivity: in B6/SJL F1 mice, transcript levels for Vbeta5.1, Vbeta8.2, and Vbeta12 are half that seen in 129/B6 mutants which have bi-allelic expression, indicating transcription correlates with number of alleles present; the Vbeta gene in SJL mice contains an 80 kp deletion from Vbeta5.2-9

immune system

abnormal T cell differentiation ( J:121608 )

• Background Sensitivity: in B6/SJL F1 mice, transcript levels for Vbeta5.1, Vbeta8.2, and Vbeta12 are half that seen in 129/B6 mutants which have bi-allelic expression, indicating transcription correlates with number of alleles present; the Vbeta gene in SJL mice contains an 80 kp deletion from Vbeta5.2-9

Genotype
MGI:3711858

cx3

• Allelic Composition: Tcrb^tm2Slek/Tcrb^tm2Slek; Trbv13-2^tm1(CD2)Zhu/Trbv13-2^tm1(CD2)Zhu
• Genetic Background: involves: 129 * C57BL/6

hematopoietic system

abnormal T cell differentiation ( J:121608 )

• human CD2 expression (germline transcription from the Vbeta8.2 promoter) is higher in cells expressing Vbeta genes downstream of Vbeta8.2 promoter, whereas no CD2 expression is detected from T cells using V genes upstream of Vbeta8.2

immune system

abnormal T cell differentiation ( J:121608 )

• human CD2 expression (germline transcription from the Vbeta8.2 promoter) is higher in cells expressing Vbeta genes downstream of Vbeta8.2 promoter, whereas no CD2 expression is detected from T cells using V genes upstream of Vbeta8.2