Analysis Tools
reproductive system
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• females are fertile and have no obvious ovarian abnormalities
• XY bodies form normally in null males
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• some sex chromatin defects are seen in mutants; some cells reaching diplonema have sex chromatin lacking histone H3K9 di- and tri-methylation
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• in adult testis, primordial germ cells and spermatogonia are abnormally organized in seminiferous tubules compared to controls at P26
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• germ cells are arrested in pachynema and later stages of germ cells are largely absent
• elongated spermatids are significantly lower in number in mutant testes
• mutants accumulate pachytene cells but have far less cells in late-pachytene and beyond at P26
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• at 3 weeks, testes contain significantly higher numbers of apoptotic cells (~3-fold) than controls, and similar results are found at 7 weeks
• many apoptotic cells are in the middle of the tubules, compared to controls where most are found near the periphery of the seminiferous tubules
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• testes stop growing at around P14
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• testes are ~one-third the weight of those from heterozygotes and wild-type adult littermates
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• males are deficient in postmeiotic spermatids and lack epididymal spermatozoa, although a few cells develop to the round spermatid stage
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• males are deficient in postmeiotic spermatids
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• spermatocytes in early leptotene to early-pachytene stages are also abnormally organized with many cells in center rather than periphery of tubules
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• at P21 and P42, germ cells in mutant testes are arrested in pachynema
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• males are completely infertile
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endocrine/exocrine glands
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• testes stop growing at around P14
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• testes are ~one-third the weight of those from heterozygotes and wild-type adult littermates
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cellular
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• some sex chromatin defects are seen in mutants; some cells reaching diplonema have sex chromatin lacking histone H3K9 di- and tri-methylation
|
|
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• males are deficient in postmeiotic spermatids
|
|
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• spermatocytes in early leptotene to early-pachytene stages are also abnormally organized with many cells in center rather than periphery of tubules
|
|
|
• in adult testis, primordial germ cells and spermatogonia are abnormally organized in seminiferous tubules compared to controls at P26
|
|
|
• germ cells are arrested in pachynema and later stages of germ cells are largely absent
• elongated spermatids are significantly lower in number in mutant testes
• mutants accumulate pachytene cells but have far less cells in late-pachytene and beyond at P26
|
|
|
• at P21 and P42, germ cells in mutant testes are arrested in pachynema
|
|
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• at 3 weeks, testes contain significantly higher numbers of apoptotic cells (~3-fold) than controls, and similar results are found at 7 weeks
• many apoptotic cells are in the middle of the tubules, compared to controls where most are found near the periphery of the seminiferous tubules
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