Phenotypes associated with this allele

• Allele Symbol: Cdc20^Gt(XE368)Byg
• Allele Name: gene trap XE368, BayGenomics
• Allele ID: MGI:3711105
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cdc20^Gt(XE368)Byg/Cdc20^Gt(XE368)Byg	involves: 129P2/OlaHsd * C57BL/6	MGI:3711665
ht2	Cdc20^Gt(XE368)Byg/Cdc20^tm1Jvd	involves: 129P2/OlaHsd * 129S6/SvEvTac * C57BL/6	MGI:4838299
cx3	Cdc20^Gt(XE368)Byg/Cdc20^Gt(XE368)Byg Pttg1^tm1Pzg/Pttg1^tm1Pzg	involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6	MGI:3711666

Genotype
MGI:3711665

hm1

• Allelic Composition: Cdc20^Gt(XE368)Byg/Cdc20^Gt(XE368)Byg
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality before implantation, incomplete penetrance ( J:121535 )

• two-cell embryos are never recovered after E3.5

• 22% of E1.5 embryos cultured for 18 hours arrested at the two-cell stage

embryonic lethality between implantation and somite formation, complete penetrance ( J:121535 )

• some embryos are found when analyzed at E7.5 (3/41); these are much smaller than littermates, and appear unlikely to develop further

• no homozygous embryos are recovered after E7.5

cellular

abnormal chromosome morphology ( J:121535 )

• chromosomes from E1.5 embryos cultured for 16 hours show arrest in metaphase; sister chromatides have not separated and arm cohesion remains intact

• metaphase to anaphase transition is blocked

Genotype
MGI:4838299

ht2

• Allelic Composition: Cdc20^Gt(XE368)Byg/Cdc20^tm1Jvd
• Genetic Background: involves: 129P2/OlaHsd * 129S6/SvEvTac * C57BL/6

reproductive system

reproductive system phenotype ( J:165500 )

N • male mice exhibit normal reproduction and normal levels of apoptosis in the testes

abnormal oocyte morphology ( J:165500 )

♀ • female mice produce more aneuploid oocytes than wild-type mice

abnormal male germ cell morphology ( J:165500 )

♂ • primary and secondary spermatocytes are more prone to aneuploidy compared to in wild-type mice

abnormal female meiosis ( J:165500 )

♀ • average time from metaphase entry to anaphase is twice as long as in wild-type oocytes

♀ • chromosomes are misaligned during meiosis I in oocyte compared to in wild-type mice

♀ • however, timing of germinal vesicle breakdown is normal

female infertility ( J:165500 )

♀ • 4 of 7 female mice fail to produce pups

reduced female fertility ( J:165500 )

♀ • female mice produce an average 4-fold fewer litters with an average 15-fold lower number of pups compared with wild-type mice

♀ • however, mice exhibit normal oogenesis and fertilization

cellular

abnormal oocyte morphology ( J:165500 )

♀ • female mice produce more aneuploid oocytes than wild-type mice

abnormal male germ cell morphology ( J:165500 )

♂ • primary and secondary spermatocytes are more prone to aneuploidy compared to in wild-type mice

aneuploidy ( J:165500 )

• female mice produce more aneuploid oocytes than wild-type mice

• primary and secondary spermatocytes are more prone to aneuploidy compared to in wild-type mice

abnormal female meiosis ( J:165500 )

♀ • average time from metaphase entry to anaphase is twice as long as in wild-type oocytes

♀ • chromosomes are misaligned during meiosis I in oocyte compared to in wild-type mice

♀ • however, timing of germinal vesicle breakdown is normal

maternal effect ( J:165500 )

• only 15% of embryos derived from dams form blastocysts compared with 93% of embryos collected from wild-type dams

• dams produce embryos that cease proliferating after the first cell division of preimplantation period and do not develop beyond the 4-cell stage unlike embryos from wild-type dams

• dams produce more aneuploid embryos compared with wild-type mice

Genotype
MGI:3711666

cx3

• Allelic Composition: Cdc20^Gt(XE368)Byg/Cdc20^Gt(XE368)Byg; Pttg1^tm1Pzg/Pttg1^tm1Pzg
• Genetic Background: involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6

mortality/aging

embryonic lethality before implantation, complete penetrance ( J:121535 )

• embryos arrested at the two-cell stage can be isolated at E3.5

• analysis of E1.5 embryos cultured for 18 hours showed that some embryos cannot divide further and arrest at 2 -cell stage

cellular

abnormal chromosome morphology ( J:121535 )

• unlike Cdc20-null embryos, chromosomes do not display metaphase configuration, but are scattered; sister chromatids are separated

• in E1.5 cultured embryos, two-cell embryos stay in metaphase for ~4 hours, then begin to wander away from the metaphase plates